, Volume 160, Issue 4, pp 285–290 | Cite as

Mating-type Distribution and Fertility Status in Magnaporthe grisea Populations from Argentina

  • V. F. Consolo
  • C. A. Cordo
  • G. L. Salerno


Isolates of Magnaporthe grisea causing gray leaf spot on rice were collected in Argentina and analyzed for mating distribution and fertility. One hundred and twenty-five isolates of M. grisea were collected from rice plants between 2000 and 2003. Each isolate was tested for mating type through a polymerase chain reaction based assay. All M. grisea isolates from Argentina belonged to a single mating type, MAT1.1. The fertility status of isolates was determined using controlled crosses in vitro, pairing each isolate with GUY11 and KA9 (MAT1.2 standard hermaphroditic testers). Production of perithecia was scarce among isolates of the blast pathogen since a low percentage of them (7.2%) developed perithecia with only one of the fertile tester (KA9); all crosses failed with the other tester strain. Asci and ascospores were not observed. The presence of only one mating type and the absence of female fertile isolates indicate that sexual reproduction is rare or absent in M. grisea populations associated with rice in Argentina.


Magnaporthe grisea mating type perithecia rice 


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  1. 1.
    Kato, H 1978Biological and genetic aspects in the perfect state of rice blast fungus, Pyricularia grisea Cav and its alliesMutation Breeding for Disease Resistance, Gamma Field Symposia17122Google Scholar
  2. 2.
    Zeigler, RS 1998Recombination in Magnaporthe griseaAnnu Rev Phytopathol36249276CrossRefPubMedGoogle Scholar
  3. 3.
    Levy, M, Romao, J, Marchetti, MA, Hamer, JE 1991DNA fingerprinting with a dispersed repeated sequence resolves pathotype diversity in the rice blast fungusPlant Cell395102CrossRefPubMedGoogle Scholar
  4. 4.
    Levy, M, Correa-Victoria, FJ, Zeigler, RS, Xu, S, Hamer, JE 1993Genetic diversity of the rice blast fungus in a disease nursery in ColombiaPhytopathology8314271433Google Scholar
  5. 5.
    Zeigler RS, Leong SA, Teng PS. Rice Blast Disease. UK: CAB International, Wallingford, Oxon, 1994Google Scholar
  6. 6.
    Zeigler, RS, Scott, RP, Leung, H, Bordeos, AA, Kumar, J, Nelson, RJ 1997Evidence of parasexual exchange of DNA in the rice blast fungus challenges its exclusive clonalityPhytopathology87284294Google Scholar
  7. 7.
    Goodwin, SB 1997The population genetics of PhytophtoraPhytopathology87462473Google Scholar
  8. 8.
    Glass, NL, Kuldau, GA 1992Mating type and vegetative incompatibility in filamentous ascomycetesAnnu Rev Phytopathol30201224CrossRefGoogle Scholar
  9. 9.
    Kang, S, Chumley, FC, Valent, B 1994Isolation of mating type genes of the phytopathogenic fungus Magnaporthe grisea using genomic substractionGenetics138289296PubMedGoogle Scholar
  10. 10.
    Hebert, TT 1971The perfect stage of Pyricularia griseaPhytopathology618387Google Scholar
  11. 11.
    Kato, H, Yamaguchi, T 1982The perfect stage of Pyricularia oryzae Cay. from rice plants in cultureAnn Phytopathol Soc Jpn48607612Google Scholar
  12. 12.
    Viji, G, Gnanamanickam, SS 1998Mating type distribution and fertility of Magnaporthe grisea populations from various hosts in IndiaPlant Dis823640Google Scholar
  13. 13.
    Notteghem, JL, Silue, D 1992Distribution of mating type alleles in Magnaporthe grisea populations pathogenic on ricePhytopathology82421424Google Scholar
  14. 14.
    Benkirane, R, Tajani, M, Douira, A, Selmaoui, K, Lebrar, S 1998Mating type of Magnaporthe grisea population in MoroccoPhytopathol Mediterr37119121Google Scholar
  15. 15.
    Kolmer, JA, Ellingboe, AE 1988Genetic relationships and virulence to rice in Magnaporthe griseaCan J Bot66891897Google Scholar
  16. 16.
    Yaegashi, H, Nishihara, N 1976Production of the perfect stage in Pyricularia from cereal and grassesAnn Phytopathol Soc Jpn42511515Google Scholar
  17. 17.
    Valent, B, Crawford, MS, Weaver, CG, Chumley, FG 1986Genetic studies of fertility and pathogenicity in Magnaporthe griseaIowa State J Res60569594Google Scholar
  18. 18.
    Valent, B, Leonard, F, Chumley, FJ 1991Magnaporthe grisea genes for pathogenicity an virulence identified through a series of backcrossesGenetics12787101PubMedGoogle Scholar
  19. 19.
    Park, SY, Milgroom, MG, Han, SS, Kang, S, Lee, YH 2003Diversity of pathotypes and DNA fingerprint haplotypes in populations of Magnaporthe grisea in Korea over two decadesPhytopathology93378385Google Scholar
  20. 20.
    Xu, JR, Hamer, JE 1996MAP kinase and cAMP signaling regulate infection structure formation and pathogenic growth in the rice blast fungus Magnaporthe griseaGene Dev10187194Google Scholar
  21. 21.
    Xue, C, Park, G, Choi, W, Zheng, L, Dean, RA, Xu, JR 2002Two novel fungal virulence genes specifically expressed in appresoria of the rice blast fungusPlant Cell1421072119CrossRefPubMedGoogle Scholar
  22. 22.
    Itoi, S, Mishima, T, Arase, S, Nozu, M 1983Mating behavior of Japanese isolates of Pyricularia oryzaePhytopathology73155158Google Scholar
  23. 23.
    Yaegashi, H, Yamada, M 1986Pathogenic races and mating types of Pyricularia oryzae from Soviet Union, China, Nepal, Thailand, Indonesia and ColombiaAnn Phytopathol Soc Jpn52225234Google Scholar
  24. 24.
    Leslie, JF, Klein, KK 1996Female fertility and mating type effects on effective population size and evolution in filamentous fungiGenetics144557567PubMedGoogle Scholar
  25. 25.
    Dayakar, BV, Narayanan, NN, Gnanamanickam, SS 2000Cross-compatibility and distribution of mating type allels of the rice blast fungus Magnaporthe grisea in IndiaPlant Dis84700704Google Scholar
  26. 26.
    Yaegashi, H 1977On the sexuality of blast, Pyricularia from cereals and grassesAnn Phytopathol Soc Jpn42511515Google Scholar
  27. 27.
    Tanaka, Y, Murata, N, Kato, H 1979Behavior of nuclei and chromosomes during ascus development on the mating between either rice-strain or weeping lovegrass-strain and ragi strain of PyriculariaAnn Phytopathol Soc Jpn45182191Google Scholar
  28. 28.
    Xia, JQ, Correll, JC, Lee, FN, Marchetti, MA, Rhodes, DD 1993DNA fingerprinting to examine microgeographic variation in the Magnaporthe grisea (Pyricularia grisea) population in two rice fields in ArkansasPhytopathology8310291035Google Scholar
  29. 29.
    Sivaraj R, Gnanamanickam SS, Levy M. Studies on the genetic diversity of Pyricularia grisea: a molecular approach for the managment of rice blast. In: Rice Genetics III, The International Rice Research Institute, los Baños, Philippines, 1999: 958–962Google Scholar
  30. 30.
    Tredway, LP, Stevenson, K, Burpee, LL 2003Mating type distribution and fertility status in Magnaporthe grisea populations from turfgrasses in GeorgiaPlant Dis87435441Google Scholar
  31. 31.
    Hayashi, N, Li, C, Li, J, Iwano, M, Naito, H, Yoshino, R, Kato, H 1997Distribution of fertile Magnaporthe grisea fungus pathogenic to rice in Yunan Province, ChinaAnn Phytopathol Soc Jpn63316323Google Scholar
  32. 32.
    Mekwatanakarn, P, Kositratana, W, Phromraksa, T, Zeigler, RS 1999Sexually fertile Magnaporthe grisea rice pathogens in ThailandPlant Dis83939943Google Scholar
  33. 33.
    Kumar, J, Nelson, RJ, Zeigler, RS 1999Population structure and dynamics of Magnaporthe grisea in the Indian HimalayasGenetics152971984PubMedGoogle Scholar
  34. 34.
    Priyadarisini, VB, Viji, G, Gnanamanickam, SS 1999Mating type distribution, fertility and pathogenicity of rice isolates of Magnaporthe grisea in four rice growing regions of IndiaPhytopathology522834Google Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  1. 1.Centro de Investigaciones BiológicasFIBAMar del PlataArgentina
  2. 2.CIDEFI, Facultad de Ciencias Agrarias y ForestalesUniversidad Nacional de La PlataLa PlataArgentina

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