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Plasmid distribution in European Diaporthe helianthi isolates

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Abstract

Diaporthe helianthi is the causal agent of sunflower stem canker, a serious pathogen of sunflower in Europe, which has been sporadically recorded in Italy. A collection of 26 Diaporthe helianthi isolates deriving from different geographic origins was analysed in order to determine the presence of extra-chromosomal genetic determinants and their molecular diversity. Extra-chromosomal bands in total genomic DNAs were identified in every French and the Yugoslavian isolate and in only one Italian isolate, while no Romanian and Argentinean isolates resulted to host any plasmids. When tested for their chemicophysical nature, they were recognised as linear plasmids sized about 2.3 Kb. A more detailed analysis was performed on a plasmid purified from a French isolate (plasmid F). Its intracellular localisation resulted as mitochondrial. Plasmid F was also exploited as a probe in Southern hybridisation experiments, in which it recognised only plasmids present in the genomes of French and Yugoslavian isolates (countries were the disease has a heavy incidence) indicating a strong correlation to geographic origin. An RFLP hybridisation analysis performed on genomic DNAs revealed a homogeneous restriction pattern in all French and Yugoslavian isolates, suggesting molecular homology among plasmids present in those isolates.

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Authors and Affiliations

  1. Dipartimento di Coltivazione e Difesa delle Specie Legnose “G. Scaramuzzi” Sez. Patologia Vegetale, Via del Borghetto 80, 56124, Pisa, Italy

    Mariarosaria Vergara, Tiziana Capasso & Giovanni Vannacci

  2. Scuola Normale Superiore di Pisa, Piazza dei Cavalieri 7, 56100, Pisa, Italy

    Mariarosaria Vergara

  3. Dipartimento di Biologia Applicata alla Difesa delle Piante, Università di Udine, Via Scienze 208, Udine, I-33100, Italy

    Emanuela Gobbi

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  1. Mariarosaria Vergara
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  2. Tiziana Capasso
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  3. Emanuela Gobbi
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  4. Giovanni Vannacci
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Correspondence to Mariarosaria Vergara.

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Vergara, M., Capasso, T., Gobbi, E. et al. Plasmid distribution in European Diaporthe helianthi isolates. Mycopathologia 159, 591–599 (2005). https://doi.org/10.1007/s11046-005-1327-0

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  • DOI: https://doi.org/10.1007/s11046-005-1327-0

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