, Volume 160, Issue 1, pp 51–62 | Cite as

Potential of oil-based formulations of Beauveria bassiana to control Triatoma infestans



The in vitro development of Beauveria bassiana conidia was monitored when immersed in six concentrations of seven non-ionic (MP 6400, MP 600, Renex 60, Renex 95, Span 80, Tween 20 and Tween 80) and three anionic (DOS 75, Hostapaval BVQ 9 and Surfax 220) surfactants and 11 vegetable oils (linseed, soybean, groundnut, rapeseed, thistle, sunflower, olive, sesame, corn, castor, and babassu). The influence of the oils on the settling behavior of Triatoma infestans nymphs and the activity of an oil–water formulation of the fungus against this vector under laboratory and simulated field conditions were also determined. With exception of DOS 75 and Surfax 220 germination of conidia on complete medium was >98% at 24 h after exposure to surfactants up to 10%. Elevated rates of germination (>25%) were observed in 10% corn, thistle and linseed oil 8 days after incubation. Pure oils had a significant repellent effect to T. infestans. Repellency decreased generally at 10% of the oil and some oils showed some attractiveness for nymphs when tested at 1%. Nymphs were highly susceptible to oil–water formulated conidia, even at unfavorable moisture for extra-tegumental development of the fungus on the insect cuticle.


Beauveria bassiana behavior formulation surfactant Triatoma infestans vegetable oil 


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  1. 1.
    Luz, C, Tigano, MS, Silva, IG, Cordeiro, CMT, Aljanabi, SM. 1998Selection ofBeauveria bassiana and Metarhizium anisopliae to control Triatoma infestansMem Inst Oswaldo Cruz93839846CrossRefPubMedGoogle Scholar
  2. 2.
    Luz, C, Silva, IG, Magalhães, BP, Cordeiro, CMT, Tigano, MS. 1999Control of Triatoma infestans (Reduviidae, Triatominae) with Beauveria bassiana: preliminary assays on formulation and application in the fieldAn Soc Entomol Bras28101110Google Scholar
  3. 3.
    Lecuona, RE, Edelstein, JD, Berretta, MF, Rossa, FR, Argas, JA. 2001Evaluation of Beauveria bassiana (Hyphomycetes) strains as potential agents for control of Triatoma infestans (Hemiptera: Reduviidae)J Med Entomol38172179PubMedGoogle Scholar
  4. 4.
    Luz, C, Rocha, LFN, Humber, RA. 2003Record of Evlachovaea sp. (Hyphomycetes) on Triatoma sordida in the State of Goiás, Brazil, and its activity against Triatoma infestans (Reduviidae, Triatominae)J Med Entomol40451454PubMedGoogle Scholar
  5. 5.
    Luz, C, Rocha, LFN, Silva, IG. 2004Pathogenicity of Evlachovaea sp. (Hyphomycetes), a new species isolated from Triatoma sordida, in Chagas’ disease vectorsRev Soc Bras Med Trop37189191PubMedGoogle Scholar
  6. 6.
    Luz, C, Rocha, LFN, Nery, GV. 2004Detection of entomopathogenic fungi in peridomestic triatomine-infested areas in Central Brazil and fungal activity against Triatoma infestans (Klug) (Hemiptera, Reduviidae)Neotropical Entomol33783791Google Scholar
  7. 7.
    Luz, C, Fargues, J, Romaña, CA, Moreno, J, Goujet, R, Rougier, M, Grunewald, J. 1994Potential of entomopathogenic hyphomycetes for the control of the triatomine vectors of Chagas’ diseaseProc 6 Int Coll Invertebr Path Microbiol Control1272276Google Scholar
  8. 8.
    Luz, C, Rocha, LFN, Nery, GV, Magalhães, BP, Tigano, MS. 2004Activity of oil-formulated Beauveria bassiana against Triatoma sordida in peridomestic areas in Central BrazilMem Inst Oswaldo Cruz99211218PubMedGoogle Scholar
  9. 9.
    Bateman, RP, Carey, M, Moore, D, Prior, C. 1993The enhanced infectivity of Metarhizium flavoviride in oil formulations to desert locusts at low humiditiesAnn Appl Biol122145152Google Scholar
  10. 10.
    Feng, MG, Poprawski, TJ, Khachatourians, GG. 1994Production, formulation and application of the entomopathogenic fungus Beauveria bassiana for insect control: current statusBiocontrol Sci Technol4334Google Scholar
  11. 11.
    Lomer, CJ, Bateman, RP, Johnson, DL, Langewald, J, Thomas, M. 2001Biological control of locusts and grasshoppersAnnu Rev Entomol46667702CrossRefPubMedGoogle Scholar
  12. 12.
    Batta, YA. 2003Production and testing of novel formulations of the entomopathogenic fungus Metarhizium anisopliae (Metschnikoff) Sorokin (Deuteromycotina: Hyphomycetes)Crop Protection22415422CrossRefGoogle Scholar
  13. 13.
    Luz, C, Fargues, J. 1997Temperature and moisture requirements for conidial germination of an isolate of Beauveria bassiana, pathogenic to Rhodnius prolixusMycopathologia138117125CrossRefGoogle Scholar
  14. 14.
    Silva, EC, Soares, IC. 1996Tecnologia de emulsõesCosmetics Toiletries83746Google Scholar
  15. 15.
    Silva, IG. 1985Influência da temperatura na biologia de triatomíneos. I. Triatoma rubrovaria (Blanchard, 1834) (Hemiptera, Reduviidae)Rev Goiana Med342934Google Scholar
  16. 16.
    Winston, PW, Bates, DH. 1960Saturated solutions for the control of humidity in biological researchEcology41232237Google Scholar
  17. 17.
    SAS Institute Inc. What’s New in SAS® Software for Release 8.1, Cary, N. C., SAS Institute Inc. 2000.Google Scholar
  18. 18.
    Baillie, AJ, Al-Assadi, H, Florence, AT. 1989Influence of nonionic surfactant structure on mobility inhibition of Tetrahymena elliotti, a model for surfactant–membrane interactionsInt J Pharm53241248CrossRefGoogle Scholar
  19. 19.
    Garon, D, Krivobok, S, Wouessidjewe, D, Murandi, FS. 2002Influence of surfactants on solubilization and fungal degradation of fluoreneChemosphere47303309CrossRefPubMedGoogle Scholar
  20. 20.
    Prasad, R. 1993Role of adjuvants in modifying the efficacy of a bioherbicide on forest species: Compatibility studies under laboratory conditionsPesticide Sci38273275Google Scholar
  21. 21.
    Abbas, HK, Egley, GH. 1996Influence of unrefined corn oil and surface-active agents on the germination and infectivity of Alternaria helianthiBiocontrol Sci Technol6531538CrossRefGoogle Scholar
  22. 22.
    Uziel, A, Kenneth, RG. 1999Influence of commercially derived lipids and a surfactant on the mode of germination and process of germ-tube formation in primary conidia of two species on Erynia subgenus Neopandora (Zygomycotina: Entomophthorales)Mycopathologia144153163CrossRefGoogle Scholar
  23. 23.
    Bidochka, MJ, Khachatourians, GG. 1992Growth of the entomopathogenic fungus Beauveria bassiana on cuticular components from the migratory grasshopper, Melanoplus sanguinipesJ Invertebr Pathol59165173CrossRefGoogle Scholar
  24. 24.
    Uttam, JR, Pandey, ND, Verma, RA, Singh, DR. 2002Efficacy of different indigenous oils as grain protectant against Sitophilus oryzae on barleyIndian J Entomol64447450Google Scholar
  25. 25.
    Pless, CD, Deyton, DE, Sams, CE. 1995Control of San Jose scale, terrapin scale, and European red mite on dormant fruit trees with soybean oilHort Sci309497Google Scholar
  26. 26.
    Liu, TX, Stansly, PA, Liu, TX. 2000Insecticidal activity of surfactants and oils against silverleaf whitefly (Bemisia argentifolii) nymphs (Homoptera: Aleyrodidae) on collards and tomatoPest Manage Sci56861866CrossRefGoogle Scholar
  27. 27.
    Fenigstein, AM, Eliyahu, MS, Veierov, D. 2001Effects of five vegetable oils on the sweetpotato whitefly Bemisia tabaciPhytoparasitica29197206Google Scholar
  28. 28.
    Moustafa, OK, Abou-Yousef, HM, El-Attal, ZM. 2002Efficiency of three types of oils against Aphis fabae and Tetranychus urticaeEgyptian J Agricult Res8011331140Google Scholar
  29. 29.
    Begum, S, Quiniones, AC. 1990Protection of stored mungbean seeds from bean weevil Callosobruchus chinensis by vegetable oil applicationBangladesh J Zool18203210Google Scholar
  30. 30.
    Pacheco, IA, Castro, MFPPM, Paula, DC, Lourenção, AL, Bolonhezi, S, Barbieri, MK. 1995Efficacy of soybean and castor oils in the control of Callosobruchus maculatus (F.) and Callosobruchus phaseoli (Gyllenhal) in stored chick-peas (Cicer arietinum L)J Stored Products Res31221228CrossRefGoogle Scholar
  31. 31.
    Dhulia, FK, Patel, AJ, Patel, IS. 1999Efficacy of various vegetable oils for protecting greengram (Vigna radiata LWilkzek) against pulse beetle in storage. Pest Manage Econ Zool2177179Google Scholar
  32. 32.
    Gupta, AK, Behal, SR, Awasthi, BK, Verma, RA. 2000Efficacy of different vegetable oils against Sitophilus oryzae in maize grainInd J Entomol62301303Google Scholar
  33. 33.
    Singh, PK. 2003Effect of some oils against pulse beetle, Callosobruchus chinensis in infesting pigeon peaIndian J␣Entomol655558Google Scholar
  34. 34.
    Ansari, MA, Razdan, RK. 1995Relative efficacy of various oils in repelling mosquitoesIndian J Malariol32104111PubMedGoogle Scholar
  35. 35.
    Luz, C, Fargues, J. 1999Dependence of the entomopathogenic fungus Beauveria bassiana, on high humidity for infection of Rhodnius prolixusMycopathologia1463341CrossRefPubMedGoogle Scholar
  36. 36.
    Fargues, J, Luz, C. 2000Effects of fluctuating moisture and temperature regimes on the infection potential of Beauveria bassiana for Rhodnius prolixusJ Invertebr Pathol75202211CrossRefPubMedGoogle Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  1. 1.DMIPP, Instituto de Patologia Tropical e Saúde PúblicaUniversidade Federal de Goiás (UFG)GoiâniaBrazil

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