Advertisement

Mycopathologia

, Volume 159, Issue 1, pp 79–85 | Cite as

Identification and pathogenic characterization of endophytic Fusarium species from cowpea seeds

  • A. A. C. Rodrigues
  • Maria Menezes
Article

Abstract

Isolates of Fusarium were obtained and identified from seeds of cowpea, Vigna unguiculata (L.) Walp., by means of blotter tests and slide cultures. Species were differentiated according to the morphology of the macroconidia, microconidia and their arrangement in chains or false heads, the size and type of conidiophore, and the presence or absence of chlamydospores. The species were identified as F. semitectum, F. equiseti, F. oxysporum, F. solani, F. anthophilum, F. sporotrichioides, F. moniliforme, and Fusarium sp. Among the species, F. semitectum was the most frequently detected. None of these species were pathogenic when inoculated in susceptible cowpea cultivar (BR 17- Gurgueia). But, an isolate of F. oxysporum f. sp. tracheiphilum used as a standard of comparison for pathogenicity (control) induced symptoms of yellowing, vascular wilting, and death of a susceptible cowpea cultivar under the same environmental conditions.

Keywords

cowpea cultivars Fusarium seed Vigna unguiculata 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Burgess, LW 1981General ecology of the FusariaNelson, PEToussoun, TACook, RJ eds. Fusarium disease, biology, and taxonomy.Pensylvania University PressUniversity Park/LondonGoogle Scholar
  2. Booth, C. 1971The genus Fusarium.Commonwealth Mycological InstituteKewGoogle Scholar
  3. Appel, DJ, Gordon, TR 1994Local e regional variation in populations sof Fusarium oxysporum from agricultural field soilsPhytopathology84786791Google Scholar
  4. Edel, V., Steinberg, C., Gautheron, N., Alabouvette, C. 1997Populations of non pathogenic Fusarium oxysporum associated with roots of four plant species compared to soilborne populationsPhytopathology87693697Google Scholar
  5. Ramachandran, P., Summanwar, AS., Lal, SP. 1982Cowpea top. Necrosis-caused by Fusarium equiseti (Corda) Sacc. Current Science51475477Google Scholar
  6. Nelson, BD., Hansen, JM. 1997Reaction of soybean cultivares to isolates of Fusarium solani from the Red River ValleyPlant Disease81664668Google Scholar
  7. Gordon, TR., Martyn, RD. 1997The evolutionary biology of Fusarium oxysporumAnnu Rev Phytopathol35111128Google Scholar
  8. Kendrick, JB. 1931Seed transmission of cowpea wiltPhytopathology21979983Google Scholar
  9. Kendrick, JB., Snyder, WC. 1942Fusarium yellows of beansPhytopathology3210101014Google Scholar
  10. Pio-Ribeiro, G., Assis-Filho, FM. 1997Doenças do caupi.Kimati, H.Amorim, L.Bergamin Filho, A.Carmargo, LEA.Resende, JAM eds. Manual de FitopatologiaSão PauloAgronomica Ceres.Google Scholar
  11. Armstrong, GM., Armstrong, JK. 1950Biological races of Fusarium causing wilt of cowpeas and soybeansPhytopathology40181193Google Scholar
  12. Armstrong, GM., Armstrong, JK. 1965Wilt of chrysanthemum caused by Race 1 of the cowpea FusariumPlant Disease49673676Google Scholar
  13. Hare, WW. 1953A new race of Fusarium causing wilt of cowpeaPhytopathology43291Google Scholar
  14. Menezes, M. 1988Aspectos diagnósticos na detecção de Fusarium em sementes. 3o Simpósio Brasileiro de Patologia de SementesFundação Cargill., Anais.São PauloGoogle Scholar
  15. Champion, R. 1997Identifier les champignons transmis par les semences.INRAParisGoogle Scholar
  16. Agarwal, VK., Sinclair, JB. 1997Principles of seed pathology.CRC Press.Bocca Raton., FLGoogle Scholar
  17. Neergaard, P. 1979Seed pathologyMacMillan Press.UK:London.Google Scholar
  18. Maude, RB. 1996Seedborne diseases and their control: Principles and practice.CAB InternationalCambridgeGoogle Scholar
  19. Windels, CE 1993FusariumSingleton, LL.Mihail, JD.Rush, CM. eds. Methods for research on soil borne phytopathogenic fungiCAPS Press.MinnesotaGoogle Scholar
  20. Tuite J. (1940) Plant pathological methods: Fungi and bacteria. Minnesota: Burgess Publishing Co.., 1969. 21. Snyder WC., Hansen HN. The species concept in Fusarium. Amer J Botany 27: 67-80.Google Scholar
  21. Snyder, WC, Hansen, HN. 1940The species concept in FusariumAmer J Botany276780Google Scholar
  22. Booth, C. 1977Fusarium Laboratory guide identification of the major species.Commonwealth Mycological Institute.KewGoogle Scholar
  23. Gerlach W., Nirenberg H. The genus Fusarium: A pictorial atlas. Mitt Biol Bundesanst Land-Forstw., 1982; 209.Google Scholar
  24. Nelson, PE, Toussoun, TA, Marasas, WFO 1983Fusarium species:An illustrated manual for identificationPensylvania State University PressUniversity Park/LondonGoogle Scholar
  25. Armstrong, GM., Armstrong, JK. 1948Nonsusceptible host as carries of wilt FusariaPhytopathology38808826Google Scholar
  26. Menezes M. Relações entre Fusarium oxysporum f. sp. vasinfectum (ATK) Snyd. & Hans. e diferentes hospedeiros não susceptŠveis [Dissertação]. Piracicaba., São Paulo: Escola Superior de Agricultura Luiz de Queiroz/USP., 1972; 48Google Scholar
  27. Messiaen, CM., Cassini, R. 1981Taxonomy of Fusarium.Nelson, PE.Toussoun, TA.Cook, RJ. eds. Fusarium disease., biology., and taxonomyPennsylvania University PressUniversity Park/LondonGoogle Scholar
  28. Shama, S., Raghunathan, NA., Shetty, HS. 1988Seed mycoflora of cowpea (Vigna unguiculata (L.) Walp.) and their pathogenic importanceSeed Sci & Technol16541548Google Scholar
  29. Jindal, KK., Thind, BS. 1990Microflora of cowpea seeds and its significance in the biological control of seedborne infection of Xanthomonas campestris pvVignicola. Seed Sci & Technol18393403Google Scholar
  30. Barros ST. Fungos de sementes de feijão macassar., Vigna unguiculata (L.) Walp. Recife: Ed. Universitária., 1981.Google Scholar
  31. Machardy WE., Beckman CH. Vascular wilt Fusaria: Infection and pathogenesis. In: Nelson PE., Toussoun TA., Cook RJ., eds. Fusarium disease., biology., and taxonomy. University Park/London: Pennsylvania University Press., 1981.Google Scholar
  32. Schroth, MN. 1962Hendrix FFJR Influence of non susceptible plants on the survival of Fusarium solani f. sp. phaseoli in soil.phytopathology52906909Google Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • A. A. C. Rodrigues
    • 1
    • 2
  • Maria Menezes
    • 1
  1. 1.Fitossanidade, Departamento de AgronomiaUniversidade Federal Rural de PernambucoRecife/PEBrasil
  2. 2.Departamento de AgronomiaUniversidade Federal Rural de PernambucoRecifeBrasil

Personalised recommendations