Pakistan ranked highest with reference to average temperatures in cotton growing areas of the world. The heat waves are becoming more intense and unpredictable due to climate change. Identification of heat tolerant genotypes requires comprehensive screening using molecular, physiological and morphological analysis. Heat shock proteins play an important role in tolerance against heat stress. In the current study, eight heat stress responsive factors, proteins and genes (HSFA2, GHSP26, GHPP2A, HSP101, HSC70-1, HSP3, APX1 and ANNAT8) were evaluated morphologically and physiologically for their role in heat stress tolerance. For this purpose, cotton crop was grown at two temperature conditions i.e. normal weather and heat stress at 45 °C. For molecular analysis, genotypes were screened for the presence or absence of heat shock protein genes. Physiological analysis of genotypes was conducted to assess net photosynthesis, stomatal conductance, transpiration rate, leaf-air temperature and cell membrane stability under control as well as high temperature. The traits photosynthesis, cell membrane stability, leaf-air temperature and number of heat stress responsive factors in each genotypes showed a strong correlation with boll retention percentage under heat stress. The genotypes with maximum heat shock protein genes such as Cyto-177, MNH-886, VH-305 and Cyto-515 showed increased photosynthesis, stomatal conductance, negative leaf-air temperature and high boll retention percentage under heat stress condition. These varieties may be used as heat tolerant breeding material.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Xu W, Zhou Z, Zhan D, Zhao W, Meng Y, Chen B, Liu W, Wang Y (2020) The difference in the formation of thermotolerance of two cotton cultivars with different heat tolerance. Arch Agron Soil Sci 66:158–169
Reddy KR, Hodges HF, McKinion JM (1997) A comparison of scenarios for the effect of global climate change on cotton growth and yield. Aust J Plant Physiol 24:707–713
Reddy KR, Brand D, Wijewardana C, Gao W (2017) Temperature effects on cotton seedling emergence, growth, and development. Agron J 109:1379–1387
Li X, He X, Smith R, Choat B, Tissue D (2020) Temperature alters the response of hydraulic architecture to CO2 in cotton plants (Gossypium hirsutum). Environ Exp Bot 172:104004
Iqbal M, Ul-Allah S, Naeem M, Ijaz M, Sattar A, Sher A (2017) Response of cotton genotypes to water and heat stress: from field to genes. Euphytica 213:1–11
Loka DA, Oosterhuis DM, Baxevanos D, Noulas C, Hu W (2020) Single and combined effects of heat and water stress and recovery on cotton (Gossypium hirsutum L.) leaf physiology and sucrose metabolism. Plant PhysiolBiochem 148:166–179
Virk G, Snider JL, Pilon C (2020) Associations between first true leaf physiology and seedling vigor in cotton under different field conditions. Crop Sci 60:404–418
Zhang S, Fu W, Zhang Z, Fan Y, Liu T (2017) Effects of elevated CO2 concentration and temperature on some physiological characteristics of cotton (Gossypium hirsutum L.) leaves. Environ Exp Bot 133:108–117
Sabagh AE, Hossain A, Islam MS, Barutcular C, Ratnasekera D, Gormus O, Tariq M (2020) Drought and heat stress in cotton (Gossypium hirsutum L.): consequences and their possible mitigation strategies. Agronomic Crops. Springer, Singapore, pp 613–634
Ahmad A, Ilyas MZ, Aslam Z, Roman M, Ali A, Naeem S, Nazar M, Rehman SU (2020) Physiological screening of cotton (Gossypium hirsutum L.) genotypes against drought tolerance. Pure ApplBiol 9:140–147
Abro S, Rajput MT, Khan MA, Sial MA, Tahir SS (2015) Screening of cotton (Gossypium hirsutum L.) genotypes for heat tolerance. Pak J Bot 47:2085–2091
Jamil A, Khan SJ, Ullah K (2020) Genetic diversity for cell membrane thermostability, yield and quality attributes in cotton (Gossypium hirsutum L.). Genet Resour Crop Evol 67:1405–1414
Karademir E, Karademir C, Sevilmis U, Basal H (2018) Correlations between canopy temperature, chlorophyll content and yield in heat tolerant cotton (Gossypium hirsutum L.) genotypes. Fresen Environ Bull 27:5230–5237
Hasanuzzaman M, Hossain MA, da Silva JAT, Fujita M (2012) Plant response and tolerance to abiotic oxidative stress: antioxidant defense is a key factor. Crop stress and its management: perspectives and strategies. Springer, Dordrecht, pp 261–315
Hasanuzzaman M, Nahar K, Alam M, Roychowdhury R, Fujita M (2013) Physiological, biochemical, and molecular mechanisms of heat stress tolerance in plants. Int J MolSci 14:9643–9684
Fender SE, O’Connell MA (1989) Heat shock protein expression in thermotolerant and thermosensitive lines of cotton. Plant Cell Rep 8:37–40
Momcilovic I, Ristic Z (2007) Expression of chloroplast protein synthesis elongation factor, EF-TU, in two lines of maize with contrasting tolerance to heat stress during early stages of plant development. J Plant Physiol 164:90–99
Haq S, Khan A, Ali M, Gai WX, Zhang HX, Yu QH, Gong ZH (2019) Knockdown of CaHSP60-6 confers enhanced sensitivity to heat stress in pepper (Capsicum annuum L.). Planta 250:2127–2145
Sable A, Rai MK, Choudhary A, Yadav VK, Agarwal SK, Sawant SV (2018) Inhibition of Heat Shock proteins HSP90 and HSP70 induce oxidative stress, suppressing cotton fiber development. Sci Rep 8:1–17
Usman MG, Rafii MY, Ismail MR, Malek MA, Latif MA, Oladosu Y (2014) Heat shock proteins: functions and response against heat stress in plants. Int J Sci Res 3(11):204–218
Zhang J, Lu Y, Cantrell RG, Hughs E (2005) Molecular marker diversity and field performance in commercial cotton cultivars evaluated in the southwestern USA. Crop Sci 45:1483–1490
Burke JJ, Wanjura DF (2001) Opportunities for improving cotton’s tolerance to high temperature. ProcBeltwide Cotton ConfNati Cotton Counc Memphis TN 1453:54
Young TE, Ling J, Geisler-Lee CJ, Tanguay RL, Caldwell C, Gallie DR (2001) Developmental and thermal regulation of the maize heat shock protein, Hsp101. Plant Physiol 127:777–791
Hong SW, Vierling E (2000) Mutants of Arabidopsis thaliana defective in the acquisition of tolerance to high temperature stress. ProcNatlAcadSci USA 97:4392–4397
Nishizawa A, Yabuta Y, Yoshida E, Maruta T, Yoshimura K, Shigeoka S (2006) Arabidopsis heat shock transcription factor A2 as a key regulator in response to several types of environmental stress. Plant J 48:535–547
Yoshida T, Ohama N, Nakajima J, Kidokoro S, Mizoi J, Nakashima K, Maruyama K et al (2011) Arabidopsis HsfA1 transcription factors function as the main positive regulators in heat shock-responsive gene expression. Mol Genet Genom 286:321–332
Shi WM, Muramoto Y, Ueda A, Takabe T (2001) Cloning of peroxisomalascorbate peroxidase gene from barley and enhanced thermotolerance by overexpressing in Arabidopsis thaliana. Gene 273:23–27
Cantero A, Barthakur S, Bushart TJ, Chou S, Morgan RO, Fernandez MP, Clark GB, Roux SJ (2006) Expression profiling of the Arabidopsisannexin gene family during germination, de-etiolation and abiotic stress. Plant PhysiolBiochem 44:13–24
Singh RP, Prasad PV, Sunita K, Giri SN, Reddy KR (2007) Influence of high temperature and breeding for heat tolerance in cotton: a review. AdvAgron 93:313–385
Zhang Z, Shang H, Shi Y, Huang L, Li J, Ge Q, Gong J et al (2016) Construction of a high-density genetic map by specific locus amplified fragment sequencing (SLAF-Seq) and its application to quantitative trait loci (QTL) analysis for boll weight in upland cotton (Gossypium hirsutum). BMC Plant Biol 16(1):1–18
Blum A, Ebercon A (1981) Cell membrane stability as a measure of drought and heat tolerance in wheat 1. Crop Sci 21:43–47
Fischer RA, Maurer R (1978) Drought resistance in spring wheat cultivars. 1. Grain-yield responses. Aust J Agric Res 29:897–912
Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15
Dewey RD, Lu KH (1959) A correlation and phenotypic correlation analysis of some quality characters and yield of seed cotton in upland cotton (Gossypium hirsutum L.). Int J BiolSci 1:235–236
Burton GW, Devane EH (1953) Estimating heritability in tall fescue (Festuca arundinacea) from replicated clonal material 1. Agron J 45(10):478–481
Cottee NS, Wilson IW, Tan DK, Bange MP (2014) Understanding the molecular events underpinning cultivar differences in the physiological performance and heat tolerance of cotton (Gossypium hirsutum). Funct Plant Biol 41:56–67
Burke JJ, Hatfield JL, Klein RR, Mullet JE (1985) Accumulation of heat shock proteins in field-grown cotton. Plant Physiol 78:394–398
Larkindale J, Vierling E (2008) Core genome responses involved in acclimation to high temperature. Plant Physiol 146:61
Maqbool A, Abbas W, Rao AQ, Irfan M, Zahur M, Bakhsh A, Riazuddin S, Husnain T (2010) Gossypium arboreum GHSP26 enhances drought tolerance in Gossypium hirsutum. BiotechnolProg 26:21–25
Qi Y, Wang H, Zou Y, Liu C, Liu Y, Wang Y, Zhang W (2011) Over-expression of mitochondrial heat shock protein 70 suppresses programmed cell death in rice. FEBS Lett. 585:231–3941
Van der Westhuizen MM, Oosterhuis DM, Berner JM, Boogaers N (2020) Chlorophyll a fluorescence as an indicator of heat stress in cotton (Gossypium hirsutum L.). S Afr J Plant Soil 37:116–119
The study was part of the NRPU research project of HEC entitled “Marker assisted gene pyramiding for heat tolerance in cotton” Project ID: 7965. We are thankful to HEC for providing funds for this research work.
Source: Higher Education Commission Islamabad, Pakistan.
Conflict of interest
The authors have no conflict of interest with any journal, institute etc.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Below is the link to the electronic supplementary material.
About this article
Cite this article
Saleem, M.A., Malik, W., Qayyum, A. et al. Impact of heat stress responsive factors on growth and physiology of cotton (Gossypium hirsutum L.). Mol Biol Rep 48, 1069–1079 (2021). https://doi.org/10.1007/s11033-021-06217-z
- Climate change
- Heat shock proteins