Genetic diversity analysis for narrow-leafed lupin (Lupinus angustifolius L.) by SSR markers

Abstract

Narrow-leafed lupin (Lupinus angustifolius L.) is used as grain legumes, fodder for livestock and green manure in the world and has a great potential to be developed as a new crop in China. In this study, we assessed the genetic diversity among a set of 109 newly introduced accessions of narrow-leafed lupin using 76 genomic SSR markers. Data analysis suggested that the average gene diversity index and average polymorphism information content (PIC) were 0.4758 and 0.4328, respectively. The mean allele number per loci (Na) was 6.3816. The population structure analysis identified two subgroups based on delta K (ΔK) values. This result is in accordance with that of a PCA. The AMOVA analysis showed that most of molecular variance were within population. These results will be useful to guide the genetic improvement of the narrow-leafed lupin crop in China.

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Acknowledgements

We acknowledge the financial support from National Infrastructure for Crop Germplasm Resources project from the Ministry of Science and Technology of China (NICGR2018), the international cooperation projects (2016-X16 and 2017YFE0105100), and also supported by Agricultural Science and Technology Innovation Program (ASTIP) in CAAS.

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TY and XXZ designed experiment and prepared the manuscript, YSJ conducted experiments and analyzed data, RL and YNH prepared all the seeds of plant material, JGH and MMR revised the manuscript, DW, GL and HYZ extracted DNA, CYW and MWL assisted in SSR genotyping.

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Correspondence to Tao Yang or Xuxiao Zong.

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Ji, Y., Liu, R., Hu, J. et al. Genetic diversity analysis for narrow-leafed lupin (Lupinus angustifolius L.) by SSR markers. Mol Biol Rep (2020). https://doi.org/10.1007/s11033-020-05596-z

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Keywords

  • Simple sequence repeats
  • Populations
  • Genetic relationships
  • PCA
  • Molecular variance