Raspberry leaf blotch emaravirus in Bosnia and Herzegovina: population structure and systemic movement

Abstract

Raspberry leaf blotch virus (RLBV) is the putative agent of the homonymous disease and even though Bosnia and Herzegovina is a major producer worldwide there is no report of the virus presence in the country. We studied the virus population structure and assessed its ability to move systemically. RLBV is widespread in production areas and has a homogeneous population structure; leading to the hypothesis that the primary mode of dissemination is propagation material. The ability of the virus to move systemically eliminates propagation of root cuttings as a viable option to obtain RLBV-free plants, leaving RT-PCR screening as the better option to propagate RLBV- free plants in the absence of clean-up facilities or certification programs in the country.

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Acknowledgements

This work was supported through Fulbright Visiting Scholar Grant at the University of Arkansas, Division of Agriculture, Department of Plant Pathology and by the Ministry of Civil Affairs of Bosnia and Herzegovina (contract number 10/33-14-632-1/17), Ministry for Scientific and Technological Development, Higher Education and Information Society of Republic of Srpska (contract number 19/6-020/966-56/18).

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All authors contributed to the discussions and agreed on key decision points. The submitted document was primarily prepared by the corresponding author and subsequently reviewed and agreed by the other authors. All authors read and approved the final version.

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Correspondence to I. E. Tzanetakis.

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Delić, D., Radulović, M., Vakić, M. et al. Raspberry leaf blotch emaravirus in Bosnia and Herzegovina: population structure and systemic movement. Mol Biol Rep 47, 4891–4896 (2020). https://doi.org/10.1007/s11033-020-05560-x

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Keywords

  • Detection
  • Emaravirus
  • Systemic movement
  • Certification
  • Population structure