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Cytotoxic and cell cycle arrest induction of pentacyclic triterpenoides separated from Lantana camara leaves against MCF-7 cell line in vitro

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Abstract

Lantana camara is an important medicinal plant that contains many active compounds, including pentacyclic triterpenoids, with numerous biological activities. The present study was conducted to evaluate the anti-oxidant, anti-tumour, and cell cycle arrest properties of chemical compounds extracted from L. camara leaves. Four compounds were identified after subjecting the plant methanolic extract to LC-MS/MS analysis: lantadene A, lantadene B, icterogenin, and lantadene C. Potential antioxidant activity was examined using 2, 2-diphenyl-1-picrylhydrazyl and compared with vitamin C as a control. Lantadene A and B were confirmed to possess the highest scavenging activity, while icterogenin and lantadene C exhibited a lesser antioxidant effect. All extracted compounds exerted a dose-dependent reduction in MCF-7 cell viability; however, lantadene B showed the highest anti-cancer activity, with an IC50 of 112.2 μg mL−1, and was therefore used in subsequent experiments. The results also confirmed the significant release of caspase 9 in a dose-dependent pattern following treatment of MCF-7 cells with a range of lantadene B concentrations. Lantadene B was found to induce MCF-7 cell cycle arrest in G1, blocking the G1/S transition with a maximum significant (p ≤ 0.01) cell count of 80.35% at 25 µg mL−1. No significant changes were observed in S phase, but a decrease in the MCF-7 population was exhibited in G2/M phase.

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Acknowledgements

The authors are grateful to the College of Biotechnology at Al-Naharin University for offering funding, space, and utilities to accomplish the project (Grant No. COB-85).

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Authors and Affiliations

  1. Department of Molecular and Medical Biotechnology, College of Biotechnology, Al-Nahrain University, Baghdad, Iraq

    Zahraa R. Shamsee & Ali Z. Al-Saffar

  2. Biotechnology Research Center, Al-Nahrain University, Baghdad, Iraq

    Ahmed F. Al-Shanon

  3. Agro-Biotechnology Institute Malaysia (ABI), C/O MARDI Headquarters, 43400, Serdang, Selangor, Malaysia

    Jameel R. Al-Obaidi

Authors
  1. Zahraa R. Shamsee
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  2. Ali Z. Al-Saffar
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  3. Ahmed F. Al-Shanon
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  4. Jameel R. Al-Obaidi
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Contributions

A-SAZ and A-SAF designed the experiments; A-SAF and SZR were involved in performing the designed experiments; A-SAF and A-OJR were responsible for analysing the data and writing and approving the manuscript.

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Correspondence to Ali Z. Al-Saffar or Jameel R. Al-Obaidi.

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Shamsee, Z.R., Al-Saffar, A.Z., Al-Shanon, A.F. et al. Cytotoxic and cell cycle arrest induction of pentacyclic triterpenoides separated from Lantana camara leaves against MCF-7 cell line in vitro. Mol Biol Rep 46, 381–390 (2019). https://doi.org/10.1007/s11033-018-4482-3

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  • DOI: https://doi.org/10.1007/s11033-018-4482-3

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