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Association of three SNPs in adiponectin gene with lipid traits of Tianzhu Black Muscovy (Cairina moschata)

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Abstract

Adiponectin plays a critically biological role in atherosclerosis, glucose utilization, lipid and carbohydrate metabolism, and triglyceride synthesis in animals and humans. However, little is known about the effect of adiponectin on lipid metabolism of the avian species. The aim of the preset study was to investigate the potential associations between adiponectin gene single nucleotide polymorphisms (SNPs) and the lipid traits in 348 females of Tianzhu Black Muscovy. Three novel SNPs (167G>A, 290T>C and 711G>A) were detected in adiponectin gene. 167G>A and 290T>C has linked very closely, and then 711G>A with 167G>A and 290T>C has no strong linkage disequilibrium, respectively. The Chi square test showed that allelic frequency and genotype frequency of two SNPs (167G>A and 711G>A) didn’t agree with the Hardy–Weinberg equilibrium (P>0.05). Four haplotypes and nine diplotypes were formed on the three SNPs of adiponectin gene. Association analysis indicated that the 167G>A genotypes were strongly associated with intramuscular fat (IMF) of chest muscle and serum total cholesterol (TC) (P < 0.01); the 290T>C genotypes were strongly associated with IMF, TC, and serum triglyceride (TG) (P < 0.01); furthermore, the 711G>A genotypes were significantly associated with TG and TC (P < 0.05); the diplotypes were strongly associated with IMF, TC, and TG (P < 0.01). Therefore, three SNPs in adiponectin were potential markers for improving IMF in Muscovy ducks.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No. 31760663) and science and technology project of Guizhou province (QKHPTRC[2017]5788).

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  1. Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University, West Campus, Huaxi District, Guiyang, 550025, Guizhou, People’s Republic of China

    Yuan-Yu Qin, Yi-Yu Zhang, Hua-Lun Luo & Lei Wu

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  1. Yuan-Yu Qin
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  2. Yi-Yu Zhang
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Correspondence to Yi-Yu Zhang.

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All of individuals were hatched on the same day, all of individuals were raised in a semi-open house and fed commercial corn–soybean diets based on the NRC requirements and subjected to same management environment in poultry research institute of Guizhou University, Guiyang, Guizhou, P.R.China. All of ducks were euthanized that the specific implementation plan was taken all experimental ducks placed in an operating room filled with a mixture of 90% argon and 10% nitrogen at 70 days of age, and then the ducks were quickly bled, and collected blood samples and dissected after they were unconscious. All experimental procedures used in this study were approved by the rules of the Institutional Animal Care and Use Committee of Guizhou University.

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Qin, YY., Zhang, YY., Luo, HL. et al. Association of three SNPs in adiponectin gene with lipid traits of Tianzhu Black Muscovy (Cairina moschata). Mol Biol Rep 46, 325–332 (2019). https://doi.org/10.1007/s11033-018-4475-2

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