Molecular Biology Reports

, Volume 46, Issue 1, pp 119–131 | Cite as

Royal jelly mitigates cadmium-induced neuronal damage in mouse cortex

  • Rafa S. AlmeerEmail author
  • Rami B. Kassab
  • Gadah I. AlBasher
  • Saud Alarifi
  • Saad Alkahtani
  • Daoud Ali
  • Ahmed E. Abdel Moneim
Original Article


This study aimed to evaluate the potential neuroprotective effect of royal jelly (RJ) against Cd-induced neuronal damage. Twenty-eight adult mice were placed equally into four groups. The control group received intraperitoneal (IP) injections of normal saline; the cadmium chloride (CdCl2) group was IP-injected 6.5 mg/kg (mg per kg of bodyweight) CdCl2; the RJ group was gavaged 85 mg/kg RJ; and the RJ + CdCl2 group was orally administered 85 mg/kg RJ 2 h before receiving IP-injections of 6.5 mg/kg CdCl2. All groups were treated for seven consecutive days and the mice were decapitated 24 h after the final dose. Cd accumulation was recorded in the cortical homogenates, accompanied by elevated levels of lipid peroxidation, nitric oxide, tumor necrosis factor-α, interleukin-1β, and the pro-apoptotic mRNA Bax and caspase-3. Meanwhile, significantly decreased levels of detoxifying antioxidant enzymes including GSH-Px, GSH-R, SOD, and CAT, anti-apoptotic mRNA Bcl-2, and monoamines such as norepinephrine, dopamine, and serotonin were also observed, along with reduced gene expression of Nrf2-dependent antioxidants. Interestingly, in mice pretreated with RJ, the assessed parameters remained near normal levels. Our data provide evidence that RJ treatment has the potential to protect cortical neurons in Cd-intoxicated mice via its antioxidant, anti-inflammatory, anti-apoptotic, and neuromodulatory activity.


Royal jelly Cadmium Neurotoxicity Mice 



The authors would like to extend their sincere appreciation to the Deanship of Scientific Research at King Saud University for its funding of this research through the research Group Project No. RGP-180.


This research was supported by the Deanship of Scientific Research at King Saud University. Research Project No. RGP-180.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11033_2018_4451_MOESM1_ESM.docx (12 kb)
Supplementary material 1 (DOCX 12 KB)


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© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Department of Zoology, College of ScienceKing Saud UniversityRiyadhSaudi Arabia
  2. 2.Department of Zoology and Entomology, Faculty of ScienceHelwan UniversityCairoEgypt

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