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Molecular Biology Reports

, Volume 46, Issue 1, pp 97–103 | Cite as

Development of a new tobamovirus-based viral vector for protein expression in plants

  • Raquel Medeiros Vasques
  • Cristiano Lacorte
  • Leonardo Lopes da Luz
  • Miguel A. Aranda
  • Tatsuya NagataEmail author
Original Article

Abstract

Plants are becoming an interesting alternative system for the heterologous production of pharmaceutical proteins, providing a more scalable, cost-effective, and biologically safer option than the current expression systems. The development of plant virus expression vectors has allowed rapid and high-level transient expression of recombinant genes, and, in turn, provided an attractive plant-based production platform. Here we report the development of vectors based on the tobamovirus Pepper mild mottle virus (PMMoV) to be used in transient expression of foreign genes. In this PMMoV vector, a middle part of the viral coat protein gene was replaced by the green fluorescent protein (GFP) gene, and this recombinant genome was assembled in a binary vector suitable for plant agroinoculation. The accumulation of GFP was evaluated by observation of green fluorescent signals under UV light and by western blotting. Furthermore, by using this vector, the multiepitope gene for chikungunya virus was successfully expressed and confirmed by western blotting. This PMMoV-based vector represents an alternative system for a high-level production of heterologous protein in plants.

Keywords

Plant viral vector Protein expression in plants Transient expression Agroinfiltration Tobamovirus 

Notes

Funding

This study was funded by the Brazilian National Council for Scientific and Technological Development, CNPq (Grant Number 401755/2013-4).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Departamento de Biologia CelularUniversidade de BrasíliaBrasíliaBrazil
  2. 2.Embrapa Recursos Genéticos e BiotecnologiaBrasíliaBrazil
  3. 3.Centro de Edafología y Biología Aplicada del Segura (CEBAS)Consejo Superior de Investigaciones Científicas (CSIC)MurciaSpain

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