Skip to main content

Advertisement

SpringerLink
Log in

ARA lncRNA, is upregulated in liver and breast tumor tissues

  • Original Article
  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Important regulatory roles of long non-coding RNAs (lncRNAs) have been recently found, and reported as useful biomarkers in cancer. To identify a potential expression of the new discovered lncRNA (ARA), during promotes cell proliferation, apoptosis inhibit, migration and cell cycle arrest, we firstly evaluate its expression in two cancer tissues (breast cancer and liver cancer) and then compared its variability expression in tumor versus non-tumor samples. Expression profile of ARA lncRNA was evaluated using qRT-PCR in paired tumor and marginal non-tumor samples collected from patients who had been referred to the Shiraz General. After RNA extraction from tissue samples, cDNA synthesis and RT-qPCR method were performed according to the protocols. ARA lncRNA expression level was calculated using 2−ΔΔCt method. Principal-component analysis followed by receiver operating characteristic curve analyses was performed to evaluate the diagnostic potential of selected lncRNA. Our data revealed a significant upregulation (P < 0.001) of ARA in breast and liver tumor tissues, in comparison to same patients non-tumor marginal samples. Also, there was a significant difference between the expression of ARA lncRNA in breast cancer and liver cancer patients (P < 0.05). In conclusion, the results of our study suggest a possible role of ARA lncRNA in proliferation of breast and liver tissues, as well as its potential usefulness as a novel diagnostic biomarker for breast and liver tumors.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Ilic M, Ilic I (2016) Epidemiology of pancreatic cancer. World J Gastroenterol 22:9694–9705. https://doi.org/10.3748/wjg.v22.i44.9694

    Article  PubMed  PubMed Central  Google Scholar 

  2. Sighoko D, Hunt BR, Irizarry B et al (2018) Disparity in breast cancer mortality by age and geography in 10 racially diverse US cities. Cancer Epidemiol 53:178–183. https://doi.org/10.1016/j.canep.2018.02.003

    Article  PubMed  PubMed Central  Google Scholar 

  3. Seyfried TN, Huysentruyt LC (2013) On the origin of cancer metastasis. Crit Rev Oncog 18:43–73

    Article  PubMed  PubMed Central  Google Scholar 

  4. Chen W, Hoffmann AD, Liu H, Liu X (2018) Organotropism: new insights into molecular mechanisms of breast cancer metastasis. NPJ Precis Oncol 2:4. https://doi.org/10.1038/s41698-018-0047-0

    Article  PubMed  PubMed Central  Google Scholar 

  5. Byler S, Goldgar S, Heerboth S et al (2014) Genetic and epigenetic aspects of breast cancer progression and therapy. Anticancer Res 34:1071–1077

    CAS  PubMed  Google Scholar 

  6. Samulin Erdem J, Notø H, Skare Ø et al (2017) Mechanisms of breast cancer risk in shift workers: association of telomere shortening with the duration and intensity of night work. Cancer Med 6:1988–1997. https://doi.org/10.1002/cam4.1135

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Kar P (2014) Risk factors for hepatocellular carcinoma in India. J Clin Exp Hepatol 4:S34–S42. https://doi.org/10.1016/j.jceh.2014.02.155

    Article  PubMed  PubMed Central  Google Scholar 

  8. Pan H, Fu X, Huang W (2011) Molecular mechanism of liver cancer. Anticancer Agents Med Chem 11:493–499

    Article  CAS  PubMed  Google Scholar 

  9. Jiang K, Al-Diffalha S, Centeno BA (2018) Primary liver cancers-part 1: histopathology, differential diagnoses, and risk stratification. Cancer Control. https://doi.org/10.1177/1073274817744625

    Article  PubMed  PubMed Central  Google Scholar 

  10. Kaikkonen MU, Lam MTY, Glass CK (2011) Non-coding RNAs as regulators of gene expression and epigenetics. Cardiovasc Res 90:430–440. https://doi.org/10.1093/cvr/cvr097

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Lin C-P, He L (2017) Noncoding RNAs in cancer development. Annu Rev Cancer Biol 1:163–184. https://doi.org/10.1146/annurev-cancerbio-050216-034443

    Article  Google Scholar 

  12. Schmitt AM, Chang HY (2016) Long noncoding RNAs in cancer pathways. Cancer Cell 29:452–463. https://doi.org/10.1016/j.ccell.2016.03.010

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Jiang M, Huang O, Xie Z et al (2014) A novel long non-coding RNA-ARA: adriamycin resistance associated. Biochem Pharmacol 87:254–283. https://doi.org/10.1016/j.bcp.2013.10.020

    Article  CAS  PubMed  Google Scholar 

  14. Malhotra A, Jain M, Prakash H et al (2017) The regulatory roles of long non-coding RNAs in the development of chemoresistance in breast cancer. Oncotarget 8:110671–110684. https://doi.org/10.18632/oncotarget.22577

    Article  PubMed  PubMed Central  Google Scholar 

  15. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2–∆∆CT method. Methods 25:402–408. https://doi.org/10.1006/meth.2001.1262

    Article  CAS  Google Scholar 

  16. Shahryari A, Rafiee MR, Fouani Y et al (2014) Two novel splice variants of ARA, ARA-S1, and ARA-S2 are coupregulated with SOX2 and OCT4 in esophageal squamous cell carcinoma. Stem Cells 32:126–134. https://doi.org/10.1002/stem.1542

    Article  CAS  PubMed  Google Scholar 

  17. Tian Y, Zhang X, Hao Y et al (2014) Potential roles of abnormally expressed long noncoding RNA UCA1 and Malat-1 in metastasis of melanoma. Melanoma Res 24:335–341. https://doi.org/10.1097/CMR.0000000000000080

    Article  CAS  PubMed  Google Scholar 

  18. He Y, Meng X-M, Huang C et al (2014) Long noncoding RNAs: novel insights into hepatocelluar carcinoma. Cancer Lett 344:20–27. https://doi.org/10.1016/j.canlet.2013.10.021

    Article  CAS  PubMed  Google Scholar 

  19. Zhou H-L, Luo G, Wise JA, Lou H (2014) Regulation of alternative splicing by local histone modifications: potential roles for RNA-guided mechanisms. Nucleic Acids Res 42:701–713. https://doi.org/10.1093/nar/gkt875

    Article  CAS  PubMed  Google Scholar 

  20. Soreq L, Guffanti A, Salomonis N et al (2014) Long non-coding RNA and alternative splicing modulations in Parkinson’s leukocytes identified by RNA sequencing. PLoS Comput Biol 10:e1003517. https://doi.org/10.1371/journal.pcbi.1003517

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Kung JTY, Colognori D, Lee JT (2013) Long noncoding RNAs: past, present, and future. Genetics 193:651–669. https://doi.org/10.1534/genetics.112.146704

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Wapinski O, Chang HY (2011) Long noncoding RNAs and human disease. Trends Cell Biol 21:354–361

    Article  CAS  PubMed  Google Scholar 

  23. Kondo Y, Shinjo K, Katsushima K (2017) Long non-coding RNAs as an epigenetic regulator in human cancers. Cancer Sci 108:1927–1933

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Cortés-Funes H, Coronado C (2007) Role of anthracyclines in the era of targeted therapy. Cardiovasc Toxicol 7:56–60. https://doi.org/10.1007/s12012-007-0015-3

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

The authors are grateful to the staffs of research deputy of Shahrekord University of Medial Sciences and Cellular and Molecular Research Center, Shahrekord University of Medical Sciences.

Author information

Authors and Affiliations

  1. Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

    Farzaneh Raeisi & Esmaeil Mahmoudi

  2. Department of Immunology, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran

    Mahdieh Abolfathi

  3. Student Research Committee, Shahrekord University of Medical Sciences, Shahrekord, Iran

    Raheleh Ahmadi-Naji

  4. Department of Immunology, Ahvaz University of Medical Sciences, Ahvaz, Iran

    Sara Iranparast

  5. Department of Biology, Faculty of Science, Fars Science and Research Branch, Islamic Azad University, Marvdasht, Iran

    Esmat Noshadi & Arvand Akbari

  6. Department of Biology, Faculty of Science, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran

    Esmat Noshadi & Arvand Akbari

  7. Medical Microbiology, Student Research Committee, Shahrekord University of Medical Sciences, Shahrekord, Iran

    Mansoor Khaledi

  8. Young Researchers and Elite Club, Najafabad Branch, Islamic Azad University, Najafabad, Iran

    Asghar Arshi

Authors
  1. Farzaneh Raeisi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mahdieh Abolfathi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Raheleh Ahmadi-Naji
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sara Iranparast
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Esmat Noshadi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Arvand Akbari
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Esmaeil Mahmoudi
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Mansoor Khaledi
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Asghar Arshi
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

FR, MA, EM and SI; literature review, manuscript writing and data collection. AA; literature review and manuscript revision, EN, AA; manuscript revision and RA-N, MK; literature review.

Corresponding author

Correspondence to Asghar Arshi.

Ethics declarations

Conflict of interest

The authors declare they have no conflict of interest.

Ethical approval

All applicable international, national, and institutional guidelines for the care of human were followed.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Raeisi, F., Abolfathi, M., Ahmadi-Naji, R. et al. ARA lncRNA, is upregulated in liver and breast tumor tissues. Mol Biol Rep 46, 77–82 (2019). https://doi.org/10.1007/s11033-018-4447-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-018-4447-6

Keywords

Search

Navigation