Molecular Biology Reports

, Volume 46, Issue 1, pp 67–76 | Cite as

Exploring nanoscale structure change of dermal tissues suffering injury by small angle X-ray scattering and transmission electron microscopy

  • Yuzhi JiangEmail author
  • Feng Tian
  • Zhiyong Wang
  • Yiwen Niu
  • Jianfei Yang
  • Fei Song
  • Shuwen Jin
  • Yemin Cao
  • Jiaoyun Dong
  • Shuliang Lu
Original Article


Scar formation and wound non-healing often occur during wound repair after skin injury, which are still unresolved. Clinic indicated that the structure played an important role in the wound repair. Our previous research showed that the wound over-healed (scar formation) when the integrity and continuity of dermal tissues was destroyed by injury. Other evidences showed that wound healing was impaired in diabetes because the underlying alternation in their skin tissues occurred caused by advanced glycation end products (AGES) aggregation. In order to explore the changes of the structure of skin at nanoscale, the small angle X-ray scattering (SAXS), compared with transmission electron microscopy (TEM), was applied to observe the skin in different pathological status. The results showed that there were some regular patterns in the structure of dermal tissue. The patterns were changed by different pathological status, which would result in wound healing disorder. These will be beneficial for clarifying the pathological mechanisms of wound healing.


Three dimensional structure Small angle X-ray scattering (SAXS) Dermal tissue Wound healing Transmission electron microscopy (TEM) 



This research was supported by the National Natural Science Foundation of China (Grant No. 81272110 and 30872685). We thank Dr. Shuwen Jin, Fei Song, Jiaoyun Dong and Jianrong Zeng for their great help.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. 1.
    Martin P (1997) Wound healing-aiming for perfect skin regeneration. Science 276(5309):75–81CrossRefGoogle Scholar
  2. 2.
    Zhou H, You C, Wang X, Jin R, Wu P, Li Q et al (2017) The progress and challenges for dermal regeneration in tissue engineering. J Biomed Mater Res A 105(4):1208–1218CrossRefPubMedGoogle Scholar
  3. 3.
    Jackson DM (1953) The diagnosis of the depth of burning. Br J Surg 40(164):588–596CrossRefPubMedGoogle Scholar
  4. 4.
    Skouge JW (1987) Techniques for split-thickness skin grafting. J Dermatol Surg Oncol 13:841–849CrossRefPubMedGoogle Scholar
  5. 5.
    Van Zuijlen PP, Ruurda JJ, van Veen HA, van Marle J, van Trier AJ, Groenevelt F et al (2003) Collagen morphology in human skin and scar tissue: no adaptations in response to mechanical loading at joints. Burns 29(5):423–431CrossRefPubMedGoogle Scholar
  6. 6.
    Dunkin CS, Pleat JM, Gillespie PH, Tyler MP, Roberts AH, McGrouther DA (2007) Scarring occurs at a critical depth of skin injury: precise measurement in a graduated dermal scratch in human volunteers. Plast Reconstr Surg 119:1722–1732CrossRefPubMedGoogle Scholar
  7. 7.
    Niu Y, Cao X, Song F, Xie T, Ji X, Miao M et al (2012) Reduced dermis thickness and AGE accumulation in diabetic abdominal skin. Int J Low Extrem Wounds 11:224–230CrossRefPubMedGoogle Scholar
  8. 8.
    Liao H, Zakhaleva J, Chen W (2009) Cells and tisue interactions with glycated collagen and their relevance to delayed diabetic wound healing. Biomaterials 30(9):1689–1696CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Vlassara H, Uribari J (2014) Advanced glycation end products (AGE) and diabetes: cause, effect, or both? Curr Diab Rep 14(1):453CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    Profyris C, Tziotzios C, Vale I (2012) Cutaneous scarring: pathophysiology, molecular mechanisms, and scar reduction therapeutics part I. The molecular basis of scar formation. J Am Acad Dermatol 66:1–10CrossRefPubMedGoogle Scholar
  11. 11.
    Jiang Y, Tong Y, Lu S (2014) Visualizing the three-dimensional mesoscopic structure of dermal tissues. J Tissue Eng Regen Med 8:794–800CrossRefPubMedGoogle Scholar
  12. 12.
    Glatter O, Kratky O (1982) Small angle X-ray scattering. Academic Press, LondonGoogle Scholar
  13. 13.
    Yang W, Sherman VR, Gludovatz B, Schaible E, Stewart P, Ritchie RO et al (2014) On the tear resistance of skin. Nat Commun 6:6649CrossRefGoogle Scholar
  14. 14.
    Zeng J, Bian F, Wang J, Li X, Wang Y, Tian F et al (2017) Performance on absolute scattering intensity calibration and protein molecular weight determination at BL16B1, a dedicated SAXS beamline at SSRF. J Synchrotron Radiat 24:509–520CrossRefPubMedGoogle Scholar
  15. 15.
    Cocera M, Rodriguez G, Rubio L, Barbosa-Barros L, Benseny-Cases N, Cladera J et al (2011) Characterisation of skin states by non-crystalline diffraction. Soft Matter 7:8605–8611CrossRefGoogle Scholar
  16. 16.
    Tang Z, Wang M, Tian F, Xu Lu, Wu G (2014) Crystal size shrinking in radiation-induced crosslinking of polytetrafluoroethylene: synchrotron small angle X-ray scattering and scanning electron microscopy analysis. Eur Polymer J 59:156–160CrossRefGoogle Scholar
  17. 17.
    Gurtner GC, Werner S, Barrandon Y, Longaker MT (2008) Wound repair and regeneration. Nature 453(7193):314–321CrossRefPubMedGoogle Scholar
  18. 18.
    Baltzis D, Eleftheriadou I, Veves A (2014) Pathogenesis and treatment of impaired wound healing in diabetes militus: new insights. Adv Ther 31(8):817–836CrossRefPubMedGoogle Scholar
  19. 19.
    Kwan PO, Tredget EE (2017) Biological priciples of scar and contracture. Hand Clin 33(2):277–292CrossRefPubMedGoogle Scholar
  20. 20.
    Brown DL, Kao WW, Greenhalgh DG (1997) Apoptosis down-regulates inflammation under the advancing epithelial wound edge: delayed patterns in diabetes and improvement with topical growth factors. Surgery 121(4):372–380CrossRefPubMedGoogle Scholar

Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Shanghai Burns Institute, Ruijin HospitalShanghai Jiaotong University School of MedicineShanghaiChina
  2. 2.Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied PhysicsChinese Academy of SciencesShanghaiChina
  3. 3.Shanghai Traditional Chinese Medicine-Integrated HospitalShanghai University of Traditional Chinese MedicineShanghaiChina
  4. 4.The Department of Burns and Plastic Surgery, Ruijin HospitalShanghai Jiaotong University School of MedicineShanghaiChina

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