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Exploring nanoscale structure change of dermal tissues suffering injury by small angle X-ray scattering and transmission electron microscopy

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Abstract

Scar formation and wound non-healing often occur during wound repair after skin injury, which are still unresolved. Clinic indicated that the structure played an important role in the wound repair. Our previous research showed that the wound over-healed (scar formation) when the integrity and continuity of dermal tissues was destroyed by injury. Other evidences showed that wound healing was impaired in diabetes because the underlying alternation in their skin tissues occurred caused by advanced glycation end products (AGES) aggregation. In order to explore the changes of the structure of skin at nanoscale, the small angle X-ray scattering (SAXS), compared with transmission electron microscopy (TEM), was applied to observe the skin in different pathological status. The results showed that there were some regular patterns in the structure of dermal tissue. The patterns were changed by different pathological status, which would result in wound healing disorder. These will be beneficial for clarifying the pathological mechanisms of wound healing.

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (Grant No. 81272110 and 30872685). We thank Dr. Shuwen Jin, Fei Song, Jiaoyun Dong and Jianrong Zeng for their great help.

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Authors and Affiliations

  1. Shanghai Burns Institute, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200025, China

    Yuzhi Jiang, Fei Song, Shuwen Jin, Jiaoyun Dong & Shuliang Lu

  2. Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201204, China

    Feng Tian

  3. Shanghai Traditional Chinese Medicine-Integrated Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200082, China

    Jianfei Yang & Yemin Cao

  4. The Department of Burns and Plastic Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200025, China

    Zhiyong Wang & Yiwen Niu

Authors
  1. Yuzhi Jiang
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  2. Feng Tian
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  3. Zhiyong Wang
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  4. Yiwen Niu
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  5. Jianfei Yang
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  6. Fei Song
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  7. Shuwen Jin
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  8. Yemin Cao
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  9. Jiaoyun Dong
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  10. Shuliang Lu
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Correspondence to Yuzhi Jiang.

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Jiang, Y., Tian, F., Wang, Z. et al. Exploring nanoscale structure change of dermal tissues suffering injury by small angle X-ray scattering and transmission electron microscopy. Mol Biol Rep 46, 67–76 (2019). https://doi.org/10.1007/s11033-018-4444-9

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  • DOI: https://doi.org/10.1007/s11033-018-4444-9

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