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Moderation of physiological responses in rice plants with Azolla under 2,4-Dichlorophenoxy acetic acid stress

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Abstract

The present work highlights some preliminary observations on metabolism in rice (Oryza sativa Linn.) when an aquatic fern Azolla pinnata R.Br. was co-cultured under 2,4-Dichlorophenoxy acetic acid toxicity. We have observed the effects of Azolla in both fresh and dried forms. This work signifies the possible physiological changes of a crop plant by using Azolla as a bioremediator. In brief the herbicide 2,4-D is considered as stressor to rice plants and by applying the fresh and dried Azolla we investigate the changes occurred. The activities of different nitrogen metabolizing enzymes and reactive oxygen species were observed. On the other hand chlorophyll and carotenoids synthesis were retrieved by addition of fresh and dried Azolla mass over 2,4-D toxicity. Thus, the efficiency of fresh and dried Azolla mass was evaluated under herbicidal toxicity in rice. We evaluate the bio remediating role of Azolla plants against 2,4-D stress and conclude this species would also be supporting in supplementation of major nutrients to rice plants.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxy acetic acid

MS media:

Murashige Skoog’s media

H2O2 :

Hydrogen peroxide

O2 − :

Superoxide

KI:

Potassium iodide

HgCl2 :

Mercuric chloride

FW:

Fresh weight

EDTA:

Ethylenediamine tetra acetic acid

DTT:

Dithiothreitol

PMSF:

Phenyl-methyl-sulfonyl-fluoride

PVPP:

Polyvinyl polypyrrolidone

MgCl2 :

Magnesium chloride

MnCl2 :

Manganese chloride

DW:

Dry weight

TCA:

Tri-chloro acetic acid

ROS:

Reactive oxygen species

References

  1. Cunningham SD, Berti WR (1993) Remediation of contaminated soils with green plants: an overview. In Vitro Cell Dev Biol 29(4):207–212

    Article  Google Scholar 

  2. Davies RJ (2000) Population trends for threatened plant species in parks and pastoral leases in South Australia. Department of Environment and Heritage

  3. Arteca RN (2013) Plant growth substances: principles and applications. Springer, New York

    Google Scholar 

  4. Mogul MG, Akin H, Hasirci N, Trantolo DJ, Gresser JD, Wise DL (1996) Controlled release of biologically active agents for purposes of agricultural crop management. Resour Conserv Recycl 16(1–4):289–320

    Article  Google Scholar 

  5. Timsina J (2005) Crop residue management for nutrient cycling and improving soil productivity in rice-based cropping systems in the tropics. Adv Agron 85:269–407

    Article  Google Scholar 

  6. Jiang L, Ma L, Sui Y, Han SQ, Wu ZY, Feng YX, Yang H (2010) Effect of manure compost on the herbicide prometryne bioavailability to wheat plants. J Hazardous Mater 184(1):337–344

    Article  CAS  Google Scholar 

  7. De AK, Sarkar B, Adak MK, Paul D, Sinha SN (2017) Physiological explanation of herbicide tolerance in Azolla pinnata R. Br. Ann Agrar Sci 15(3):402–409

    Article  Google Scholar 

  8. Gurney SE, Robinson GG (1989) The influence of two triazine herbicides on the productivity, biomass and community composition of freshwater marsh periphyton. Aquat Bot 36(1):1–22

    Article  CAS  Google Scholar 

  9. De AK, Adak ND, Adak MK (2016) Biotechnological implication with Azolla pinnata R.Br. for metal quenching ability with physiological biomarkers. Cryptogam Biodivers Assess. https://doi.org/10.21756/cba.v1i1.10960

    Article  Google Scholar 

  10. Kaizzi CK, Ssali H, Nansamba A, Vlek PL (2007) The potential benefits of Azolla, Velvet bean (Mucuna pruriens var. utilis) and N fertilizers in rice production under contrasting systems in eastern Uganda. In: Advances in integrated soil fertility management in sub-Saharan Africa: challenges and opportunities. Springer, Dordrecht, pp 423–434

  11. Mäder P, Kaiser F, Adholeya A, Singh R, Uppal HS, Sharma AK, Srivastava R, Sahai V, Aragno M, Wiemken A, Johri BN (2011) Inoculation of root microorganisms for sustainable wheat–rice and wheat–black gram rotations in India. Soil Biol Biochem 43(3):609–619

    Article  CAS  Google Scholar 

  12. Lowendorf HS (1982) Biological nitrogen fixation in flooded rice fields. Cornell University, New York

    Google Scholar 

  13. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  14. Porra RJ, Thompson WA, Kriedemann PE (1989) Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochem Biophys Acta 975:384–394

    CAS  Google Scholar 

  15. Ghosh N, Adak MK, Ghosh PD, Gupta S, Sen Gupta DN, Mandal C (2011) Differential responses of two rice varieties to salt stress. Plant Biotechnol Rep 5:89–103

    Article  Google Scholar 

  16. Achary VMM, Jena S, Panda K, Panda B (2007) Aluminium induced oxidative stress and DNA damage in root cells of Allium cepa L. Ecotoxicol Environ Saf 70:300–310

    Article  CAS  PubMed  Google Scholar 

  17. Carelli MLC, Fahl JI, Ramalho JDC (2006) Aspects of nitrogen metabolism in coffee plants. Braz J Plant Physiol 18(1):9–21

    Article  CAS  Google Scholar 

  18. Esposito S, Guerriero G, Vona V, Di Martino Rigano V, Carfagna S, Rigano C (2004) Glutamate synthase activities and protein changes in relation to nitrogen nutrition in barley: the dependence on different plastidic glucose-6P dehydrogenase isoforms. J Experim Bot 56(409):55–64

    Google Scholar 

  19. Kwinta J, Cal K (2005) Effects of salinity stress on the activity of glutamine synthetase and glutamate dehydrogenase in triticale seedlings. Pol J Environ Stud 14(1):125–130

    CAS  Google Scholar 

  20. Harvey RM, Fox JL (1973) Nutrient removal using Lemna minor. J Water Pollut Control Fed 45:1928–1938

    CAS  Google Scholar 

  21. Wani SH, Sanghera GS, Athokpam H, Nongmaithem J, Nongthongbam R, Naorem BS, Athokpam HS (2012) Phytoremediation: curing soil problems with crops. Afr J Agric Res 7(28):3991–4002

    Google Scholar 

  22. De AK, Sarkar B, Adak MK (2017) Physiological explanation of herbicide tolerance in Azolla pinnata R.Br. Ann Agrar Sci 15:402–409

    Article  Google Scholar 

  23. De AK, Bera S, Adak MA (2015) Physiological changes of duck weed fern (Azolla pinnata R. Br.) under nitrogen and phosphorus depletion, India. Genomics Appl Biol 6(9):1–16

    CAS  Google Scholar 

  24. Cathcart RJ, Swanton CJ (2004) Nitrogen and green foxtail (Setaria viridis) competition effects on corn growth and development. Weed Sci 52(6):1039–1049

    Article  CAS  Google Scholar 

  25. Yao Y, Zhang M, Tian Y, Zhao M, Zeng K, Zhang B, Zhao M, Yin B (2018) Azolla biofertilizer for improving low nitrogen use efficiency in an intensive rice cropping system. Field Crops Res 216:158–164

    Article  Google Scholar 

  26. Cruz JL, Mosquim PR, Pelacani CR, Araújo WL, DaMatta FM (2004) Effects of nitrate nutrition on nitrogen metabolism in cassava. Biol Plant 48(1):67–72

    Article  CAS  Google Scholar 

  27. Yan-Li ZH, Bo SU (2017) Nitrogen use efficiency of rice under Cd contamination: impact of rice cultivar versus soil type. Pedosphere 27:1092–1104

    Article  Google Scholar 

  28. Polle A, Schützendübel A (2003) Heavy metal signalling in plants: linking cellular and organismic responses. In: Hirt H, Shinozaki K (eds) Plant responses to abiotic stress. Springer, Berlin, pp 187–215

    Chapter  Google Scholar 

  29. Saltani N et al (2012) Urea ammonium nitrate as a carrier for herbicide in winter wheat. Am J Plant Sci 3:417

    Article  CAS  Google Scholar 

  30. Liu JJ, Wei Z, Li JH (2014) Effects of copper on leaf membrane structure and root activity of maize seedling. Bot Stud 55(1):47

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Xu G, Fan X, Miller AJ (2012) Plant nitrogen assimilation and use efficiency. Annu Rev Plant Biol 63:153–182

    Article  CAS  PubMed  Google Scholar 

  32. De AK, Dey N, Adak MK (2016) Bio indices for 2, 4-D sensitivity between two plant species: Azolla pinnata R. Br. and Vernonia cinerea L. with their cellular responses. Physiol Mol Biol Plants 22(3):371–380

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. Sunmonu TO, Van Staden J (2014) Phytotoxicity evaluation of six fast-growing tree species in South Africa. S Afr J Bot 90:101–106

    Article  CAS  Google Scholar 

  34. Sharma P, Jha AB, Dubey RS, Pessarakli M (2012) Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. J Bot. https://doi.org/10.1155/2012/217037

    Article  Google Scholar 

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Acknowledgements

The present work is financially supported by DST-PURSE programme on University of Kalyani, DST, Govt. of INDIA, New Delhi.

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Authors and Affiliations

  1. Plant Physiology and Plant Molecular Biology Research Unit, Department of Botany, University of Kalyani, Kalyani, 74 1235, India

    Arnab Kumar De, Arijit Ghosh, Kankana Biswas & M. K. Adak

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  1. Arnab Kumar De
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  2. Arijit Ghosh
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  3. Kankana Biswas
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  4. M. K. Adak
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Correspondence to M. K. Adak.

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De, A.K., Ghosh, A., Biswas, K. et al. Moderation of physiological responses in rice plants with Azolla under 2,4-Dichlorophenoxy acetic acid stress. Mol Biol Rep 46, 59–66 (2019). https://doi.org/10.1007/s11033-018-4443-x

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