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Purinoceptor expression in hepatocellular virus (HCV)-induced and non-HCV hepatocellular carcinoma: an insight into the proviral role of the P2X4 receptor

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Abstract

The basic idea behind this study was to discover the association and prevalence of purinoceptors in hepatitis C virus (HCV) and non-HCV hepatocellular carcinoma (HCC). Immunohistochemistry was performed to study the expression of P2X4 and P2X7 receptors on ex-planted liver tissue samples that were collected from HCC patients. Antibodies specific for the P2X4 and P2X7 receptors were used to target the specific receptors and secondary antibody was used with 3,3′-diaminobenzidine (DAB) detection system to visualize the color change in case of any positive expression There was a substantial increase in P2X4 receptor expression in HCV induced HCC as compared to non-HCV HCC. Surprisingly, there was no increase in the P2X7 receptor expression in both HCV HCC and non-HCV HCC. We conclude that P2X4 receptor expression was significant in the presence of HCV HCC. This may confirms the potential role of P2X4 receptor in the presence of virus in liver pathology. However insignificant expression of P2X7 receptor may avert our attention towards understanding the role of this receptor in pro-inflammatory and immune responses.

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Acknowledgements

We are thankful to higher education commission and NUST for providing the opportunity to conduct this research work. We are also thankful to Liver transplant unit of Shaikh Zayed Hospital Lahore in coordinating this research work.

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Authors and Affiliations

  1. Atta-ur-Rahman School of Applied Bio-Sciences, Department of Healthcare Biotechnology, National University of Sciences and Technology, Islamabad, 44000, Pakistan

    Madiha Khalid, Sobia Manzoor & Arun Asif

  2. HepatopancreatoBiliary Liver Transplant Unit, Shaikh Zayd Hospital, Lahore, 54000, Punjab, Pakistan

    Hassam Ahmad, Tariq Ali Bangash & Amer Latif

  3. Department of Histopathology, Shaikh Zayd Hospital, Lahore, 54000, Punjab, Pakistan

    Shahla Jaleel

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  1. Madiha Khalid
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  2. Sobia Manzoor
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  3. Hassam Ahmad
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  4. Arun Asif
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  5. Tariq Ali Bangash
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  6. Amer Latif
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Correspondence to Sobia Manzoor.

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The authors, who contributed for this review paper, do not hold any kind of the conflict of interest.

Research involving human participants and/or animals

This article involved liver biopsy samples from human participants, and does not contain any studies with animals performed by any of the authors.

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Informed consent was obtained from all individual participants included in the study.

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Khalid, M., Manzoor, S., Ahmad, H. et al. Purinoceptor expression in hepatocellular virus (HCV)-induced and non-HCV hepatocellular carcinoma: an insight into the proviral role of the P2X4 receptor. Mol Biol Rep 45, 2625–2630 (2018). https://doi.org/10.1007/s11033-018-4432-0

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  • DOI: https://doi.org/10.1007/s11033-018-4432-0

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