Skip to main content
SpringerLink
Log in

Acute responses of hemodynamic and oxidative stress parameters to aerobic exercise with blood flow restriction in hypertensive elderly women

  • Original Article
  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Systemic arterial hypertension has been associated with the majority deaths from cardiovascular disease, especially among the elderly population, and the imbalance between antioxidant and pro-oxidants has been associated with hypertension. This study analyzed the acute responses of cardiorespiratory and oxidative stress parameters to low intensity aerobic exercise (LIAE) with blood flow restriction (BFR) in hypertensive elderly women. The experimental group consisted of 16 hypertensive women (67.2 ± 3.7 years) who underwent a progressive treadmill test and performed three exercise protocols in random order: high intensity (HIAE), low intensity aerobic exercise (LIAE) and low intensity aerobic exercise with blood flow restriction (LIAE + BFR). Data analysis showed that blood pressure and heart rate augmented from rest to post effort (p < 0.05) and reduced from post effort to recovery (p < 0.05) in all protocols. The values of lipid peroxidation were higher after 30 min of recovery when compared to the moment at rest in the LILIAE + BFR (p < 0.05). The same occurred with glutathione-S-transferase and superoxide dismutase activity. However, non-protein thiols levels (NPSH) reduced after 30 min of recovery when compared to the moment at rest in the LILIAE + BFR protocol (p < 0.05). In the HIAE and LIAE + BFR protocols, the levels of NPSH were lower at 30 min of recovery when compared to the same moment in the LIAE protocol (p < 0.05). LIAE + RBF produces an oxidative status and hemodynamic stimulus similar to HIAE. Taken together, these results support the indication of LIAE with BFR in chronic intervention protocols, with potential benefits for the hypertensive elderly population.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. World Health Organization (2013) A global brief on hypertension: silent killer, global public health crisis. World Health Day 2013. World Health Organization, Geneva

    Google Scholar 

  2. Malachias MVB, Souza WKSB, Plavnik FL, Rodrigues CIS, Brandão AA, Neves MFT (2016) 7ª Diretriz brasileira de hipertensão arterial. Arq Bras Cardiol 107:1–103

    PubMed  PubMed Central  Google Scholar 

  3. Halliwell B, Gutteridge JMC (1999) Free radicals in biology and medicine. Oxford University press, New York

    Google Scholar 

  4. Slater TF (1984) Free radical mechanisms in tissue injury. Biochem J 222:1–15

    Article  CAS  Google Scholar 

  5. Hamer M, Ingle G, Carroll S, Stamatakis E (2012) Physical activity and cardiovascular mortality risk: possible protective mechanisms? Med Sci Sports Exerc 44:84–88. https://doi.org/10.1249/MSS.0b013e3182251077

    Article  PubMed  Google Scholar 

  6. Mora S, Cook N, Buring JE, Ridker PM, Lee IM (2007) Physical activity and reduced risk of cardiovascular events: potential mediating mechanisms. Circulation 116:2110–2118. https://doi.org/10.1161/CIRCULATIONAHA.107.729939

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Sato Y (2005) The history and future of Kaatsu training. Int J Kaatsu Train Res 1:1–5

    Article  Google Scholar 

  8. Schulz KF, Altman DG, Moher D (2010) CONSORT 2010 statement: updated guidelines for reporting parallel group randomized trials. Ann Int Med 152:726–732. https://doi.org/10.7326/0003-4819-152-11-201006010-00232

    Article  PubMed  Google Scholar 

  9. Ellman GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82:70–77

    Article  CAS  Google Scholar 

  10. Jacques-Silva MC, Nogueira CW, Broch LC, Flores EM, Rocha JB (2001) Diphenyl diselenide and ascorbic acid changes deposition of selenium and ascorbic acid in liver and brain of mice. Pharmacol Toxicol 88:119–125. https://doi.org/10.1034/j.1600-0773.2001.d01-92.x

    Article  CAS  PubMed  Google Scholar 

  11. Jentzsch AM, Bachmann H, Fürst P, Biesalski HK (1996) Improved analysis of malondialdehyde in human body fluids. Free Radic Biol Med 20:251–256. https://doi.org/10.1016/0891-5849(95)02043-8

    Article  CAS  PubMed  Google Scholar 

  12. Levine RL, Garland D, Oliver CN, Amici A, Climent I, Lenz AG, Ahn BW, Shaltiel S, Stadtman ER (1990) Determination of carbonyl content in oxidatively modified proteins. Methods Enzymol 186:464–478. https://doi.org/10.1016/0076-6879(90)86141-H

    Article  CAS  PubMed  Google Scholar 

  13. Miranda KM, Espey MG, Wink DA (2001) A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide 5:62–71. https://doi.org/10.1006/niox.2000.0319

    Article  CAS  PubMed  Google Scholar 

  14. Warholm M, Guthenberg C, von Bahr C, Mannervik B (1985) Glutathione transferases from human liver. Methods Enzymol 113:499–504

    Google Scholar 

  15. Misra HP, Fridovich I (1972) The role of superoxide anion in the autoxidation of epinephrine and simple assay for superoxide dismutase. J Biol Chem 247:3170–3175

    CAS  PubMed  Google Scholar 

  16. Metcalf JA, Gallin JI, Nauseef WM, Root RK (1986) Laboratory manual of neutrophil function. Raven Press, New York

    Google Scholar 

  17. Renzi CP, Tanaka H, Sugawara J (2010) Effects of leg blood flow restriction during walking on cardiovascular function. Med Sci Sports Exerc 42:726–732. https://doi.org/10.1249/MSS.0b013e3181bdb454

    Article  PubMed  PubMed Central  Google Scholar 

  18. Iida H, Kurano H, Takano H, Kubota N, Morita T, Meguro K, Sato Y, Abe T, Yamazaki Y, Uno K, Takenaka K, Hirose K, Nakajima T (2007) Hemodynamic and neurohumoral responses to the restriction of femoral blood flow by KAATSU in healthy subjects. Eur J Appl Physiol 100:275–285. https://doi.org/10.1007/s00421-007-0430-y

    Article  PubMed  Google Scholar 

  19. Ferreira MLV, Sardeli AV, Souza GV, Bonganha V, Santos LDC, Castro A, Cavaglieri CR, Chacon-Mikahil MPT (2017) Cardiac autonomic and haemodynamic recovery after a single session of aerobic exercise with and without blood flow restriction in older adults. J Sports Sci 35:2412–2420. https://doi.org/10.1080/02640414.2016.1271139

    Article  PubMed  Google Scholar 

  20. Neto GA, Pereira-Júnior PP, Mura L, Carta MG, Machado S (2015) Effects of different types of physical exercise on the perceived quality of life in active elderly. CNS Neurol Disord Drug Targets 14:1152–1156. https://doi.org/10.2174/1871527315666151111130045

    Article  CAS  PubMed  Google Scholar 

  21. Anunciação PG, Polito MD (2011) A review on post-exercise hypotension in hypertensive individuals. Arq Bras Cardiol 96:100–109. https://doi.org/10.1590/S0066-782X2011005000025

    Article  Google Scholar 

  22. Ciolac EG, Guimarães GV, D’Avila VM, Bortolotto LA, Doria EL, Bocchi EA (2009) Acute effects of continuous and interval aerobic exercise on 24-h ambulatory blood pressure in long-term treated hypertensive patients. Int J Cardiol 133:381–387. https://doi.org/10.1016/j.ijcard.2008.02.005

    Article  PubMed  Google Scholar 

  23. Moraes MR, Bacurau RF, Ramalho JD, Reis FC, Casarini DE, Chagas JR, Oliveira V, Higa EM, Abdalla DS, Pesquero JL, Pesquero JB, Araujo RC (2007) Increase in kinins on post-exercise hypotension in normotensive and hypertensive volunteers. Biol Chem 388:533–540. https://doi.org/10.1515/BC.2007.055

    Article  CAS  PubMed  Google Scholar 

  24. Fagard RH, Cornelissen VA (2007) Effect of exercise on blood pressure control in hypertensive patients. Eur J Cardiovasc Prev Rehabil 14:12–17. https://doi.org/10.1097/HJR.0b013e3280128bbb

    Article  PubMed  Google Scholar 

  25. Brandão Rondon MUP, Alves MJ, Braga AM, Teixeira OT, Barretto AC, Krieger EM, Negrão CE (2002) Postexercise blood pressure reduction in elderly hypertensive patients. J Am Coll Cardiol 39:676–682. https://doi.org/10.1016/S0735-1097(01)01789-2

    Article  PubMed  Google Scholar 

  26. Quinn TJ (2000) Twenty-four-hour, ambulatory blood pressure responses following acute exercise: impact of exercise intensity. J Hum Hypertens 14:547–553. https://doi.org/10.1038/sj.jhh.1001106

    Article  CAS  PubMed  Google Scholar 

  27. May AK, Brandner CR, Warmington SA (2017) Hemodynamic responses are reduced with aerobic compared with resistance blood flow restriction exercise. Physiol Rep 5:1–10. https://doi.org/10.14814/phy2.13142

    Article  Google Scholar 

  28. Staunton CA, Maio AK, Brandner CR, Warmington SA (2015) Haemodynamics of aerobic and resistance blood flow restriction exercise in young and older adults. Eur J Appl Physiol 115:2293–2302. https://doi.org/10.1007/s00421-015-3213-x

    Article  PubMed  Google Scholar 

  29. Sugawara J, Tomoto T, Tanaka H (2015) Impact of leg blood flow restriction during walking on central arterial hemodynamics. Am J Physiol Regul Integr Comp Physi 309:R732–R739. https://doi.org/10.1152/ajpregu.00095.2015

    Article  CAS  Google Scholar 

  30. Ozaki H, Brechue WF, Sakamaki M, Yasuda T, Nishikawa M, Aoki N, Ogita F, Abe T (2010) Metabolic and cardiovascular responses to upright cycle exercise with leg blood flow reduction. J Sports Sci Med 9:224–230

    PubMed  PubMed Central  Google Scholar 

  31. Omer K, Nermin G, Ali A, Mehmet A, Unal D, Sezen KS, Hakan K (2017) Tourniquet-induced ischaemia-reperfusion injury: the comparison of antioxidative effects of small-dose propofol and ketamine. Rev Bras Anestesiol 67:246–250. https://doi.org/10.1016/j.bjan.2016.10.007

    Article  PubMed  Google Scholar 

  32. Bouzid MA, Hammouda O, Matran R, Robin S, Fabre C (2015) Influence of physical fitness on antioxidant activity and malondialdehyde level in healthy older adults. Appl Physiol Nutr Metab 40:582–589. https://doi.org/10.1139/apnm-2014-0417

    Article  CAS  PubMed  Google Scholar 

  33. Steinberg JG, Ba A, Brégeon F, Delliaux S, Jammes Y (2007) Cytokine and oxidative responses to maximal cycling exercise in sedentary subjects. Med Sci Sports Exerc 39:964–968. https://doi.org/10.1097/mss.0b013e3180398f4b

    Article  CAS  PubMed  Google Scholar 

  34. Steinberg JG, Delliaux S, Jammes Y (2006) Reliability of different blood indices to explore the oxidative stress in response to maximal cycling and static exercises. Clin Physiol Funct Imaging 26:106–112. https://doi.org/10.1111/j.1475-097X.2006.00658.x

    Article  CAS  PubMed  Google Scholar 

  35. Laaksonen DE, Atalay M, Niskanen L, Uusitupa M, Hanninen O, Sen CK (2013) Blood glutathione homeostasis as a determinant of resting and exercise-induced oxidative stress in young. Redox Rep 1:53–59. https://doi.org/10.1179/135100099101534648

    Article  Google Scholar 

  36. Fisher-Wellman K, Bloomer RJ (2009) Acute exercise and oxidative stress: a 30-year history. Dyn Med 8:1–25. https://doi.org/10.1186/1476-5918-8-1

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  37. Michailidis Y, Jamurtas AZ, Nikolaidis MG, Fatouros IG, Koutedakis Y, Papassotiriou I, Kouretas D (2007) Sampling time is crucial for measurement of aerobic exercise-induced oxidative stress. Med Sci Sports Exerc 39:1107–1113. https://doi.org/10.1249/01.mss.0b013e318053e7ba

    Article  CAS  PubMed  Google Scholar 

  38. Powers SK, Lennon SL (1999) Analysis of cellular responses to free radicals: focus on exercise and skeletal muscle. Proc Nutr Soc 58:1025–1033

    Google Scholar 

  39. Farney TM, Mccarthy CG, Canale RE, Schilling BK, Whitehead PN, Bloomer RJ (2012) Absence of blood oxidative stress in trained men after strenuous exercise. Med Sci Sports Exerc 44:1855–1863. https://doi.org/10.1249/MSS.0b013e3182592575

    Article  CAS  PubMed  Google Scholar 

  40. Ingul CB (2018) Low volume, high intensity: time-efficient exercise for the treatment of hypertension: invited Commentary. Eur J Prev Cardiol. https://doi.org/10.1177/2047487318760040. Available at http://journals.sagepub.com.

    Article  Google Scholar 

  41. Weston KS, Wisløff U, Coombes JS (2014) High-intensity interval training in patients with lifestyle-induced cardiometabolic disease: a systematic review and meta-analysis. Br J Sports Med 48:1227–1234. https://doi.org/10.1136/bjsports-2013-092576

    Article  PubMed  Google Scholar 

Download references

Acknowledgements

We thank Unochapecó University for his financial support.

Author information

Authors and Affiliations

  1. Health Science Postgraduate Program, Unochapecó University, Servidão Anjo da Guarda Street, 295-D, Chapecó, SC, 89809-000, Brazil

    Angélica Barili, Vanessa da Silva Corralo & Clodoaldo Antônio De Sá

  2. Federal University of Fronteira Sul, Campus Chapecó, Chapecó, SC, Brazil

    Andréia Machado Cardoso, Margarete Dulce Bagatini & Gabriela Gonçalves de Oliveira

  3. Molecular Biology and Biochemistry Department, Federal University of Santa Maria, Santa Maria, RS, Brazil

    Aline Mânica

  4. Pharmacology Department, Federal University of Santa Maria, Santa Maria, RS, Brazil

    Beatriz da Silva Rosa Bonadiman

  5. Department of Physical Education, Federal University of Sergipe, Aracaju, SE, Brazil

    Marzo Edir Da Silva-Grigoletto

Authors
  1. Angélica Barili
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Vanessa da Silva Corralo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Andréia Machado Cardoso
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Aline Mânica
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Beatriz da Silva Rosa Bonadiman
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Margarete Dulce Bagatini
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Marzo Edir Da Silva-Grigoletto
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Gabriela Gonçalves de Oliveira
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Clodoaldo Antônio De Sá
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Clodoaldo Antônio De Sá.

Ethics declarations

Conflict of interest

It is an academic work and there is no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Barili, A., Corralo, V.d., Cardoso, A.M. et al. Acute responses of hemodynamic and oxidative stress parameters to aerobic exercise with blood flow restriction in hypertensive elderly women. Mol Biol Rep 45, 1099–1109 (2018). https://doi.org/10.1007/s11033-018-4261-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-018-4261-1

Keywords

Search

Navigation