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Genome survey sequencing of red swamp crayfish Procambarus clarkii

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Abstract

Red swamp crayfish, Procambarus clarkii, presently is an important aquatic commercial species in China. The crayfish is a hot area of research focus, and its genetic improvement is quite urgent for the crayfish aquaculture in China. However, the knowledge of its genomic landscape is limited. In this study, a survey of P. clarkii genome was investigated based on Illumina’s Solexa sequencing platform. Meanwhile, its genome size was estimated using flow cytometry. Interestingly, the genome size estimated is about 8.50 Gb by flow cytometry and 1.86 Gb with genome survey sequencing. Based on the assembled genome sequences, total of 136,962 genes and 152,268 exons were predicted, and the predicted genes ranged from 150 to 12,807 bp in length. The survey sequences could help accelerate the progress of gene discovery involved in genetic diversity and evolutionary analysis, even though it could not successfully applied for estimation of P. clarkii genome size.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31501858) and the Fundamental Research Funds for the Central Universities (No. 2662016QD009). The study was also financed by the 2nd batch of Modern Agroindustry Technology Research System of Hubei Province. We thank Miss Xiaoran Song and Ruijing Gen, College of fisheries, Huazhong Agriculture University, for their assistance with technique support.

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Authors and Affiliations

  1. College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Linlin Shi & Yanhe Li

  2. Aquaculture Genetics and Breeding Laboratory, The Ohio State University South Centers, 1864 Shyville Road, Piketon, OH, 45661, USA

    Shaokui Yi

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  1. Linlin Shi
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  2. Shaokui Yi
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Correspondence to Yanhe Li.

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This study was conducted with ethical approval by the Institutional Animal Care and Use Committee (IACUC) of Huazhong Agricultural University (Wuhan, China) according to the national and international guidelines.

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Shi, L., Yi, S. & Li, Y. Genome survey sequencing of red swamp crayfish Procambarus clarkii. Mol Biol Rep 45, 799–806 (2018). https://doi.org/10.1007/s11033-018-4219-3

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