Molecular Biology Reports

, Volume 45, Issue 3, pp 373–377 | Cite as

Anticancer effect of fermented Insampaedok-san in human colon cancer cells: a pilot study

  • Hwayong Park
  • Kwang Dong Kim
Short Communication


Insampaedok-san (IPS) is one of the East Asian traditional medicines which have been prescribed for hundreds of years to treat common cold and headache. Although many herbs and prescriptions are known to have significant activities against diseases, only a limited number of reports and scientific evidences on their efficacies are available. To identify anticancer effect against colon cancer, traditional prescription IPS and its fermented IPS (FIPS) were examined by in vitro molecular biological analysis. IPS water extract was fermented, lyophilized, and examined by cytotoxicity, cell cycle, and western blot assays, using cancer cell lines. Resultantly, FIPS showed significant cytotoxicities inducing caspase dependent apoptosis and activation of caspase-3 (CASP3) and poly (ADP-ribose) polymerase (PARP) cleavage in colon cancer cell line SW620. These findings can be useful for development of anticancer functional food or complementary and alternative medicine, with remaining in-depth molecular functional studies.


Colon cancer Fermentation Insampaedok-san Traditional medicine 



This work was supported by Grant (No. K13050) from the Korea Institute of Oriental Medicine funded by the Ministry of Science and ICT (Information and Communication Technologies), Republic of Korea.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interests.

Supplementary material

11033_2018_4171_MOESM1_ESM.tif (499 kb)
Supplementary material 1 (TIF 499 KB)


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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Korean Medicine Application CenterKorea Institute of Oriental MedicineDaeguRepublic of Korea
  2. 2.Division of Applied Science, PMBBRCGyeongsang National UniversityJinjuRepublic of Korea

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