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Molecular Biology Reports

, Volume 44, Issue 1, pp 183–189 | Cite as

Analysis of 12 X-STR loci in the population of south Croatia

  • Gordan Mršić
  • Petar Ozretić
  • Josip Crnjac
  • Siniša Merkaš
  • Ivana Račić
  • Sara Rožić
  • Viktorija Sukser
  • Maja Popović
  • Marina Korolija
Original Article

Abstract

The aim of the study was to assess forensic pertinence of 12 short tandem repeats (STRs) on X-chromosome in south Croatia population. Investigator® Argus X-12 kit was used to co-amplify 12 STR loci belonging to four linkage groups (LGs) on X-chromosome in 99 male and 98 female DNA samples of unrelated donors. PCR products were analyzed by capillary electrophoresis. Population genetic and forensic parameters were calculated by the Arlequin and POPTREE2 software, and an on-line tool available at ChrX-STR.org. Hardy–Weinberg equilibrium was confirmed for all X-STR markers in female samples. Biallelic patterns at DXS10079 locus were detected in four male samples. Polymorphism information content for the most (DXS10135) and the least (DXS8378) informative markers was 0.9212 and 0.6347, respectively. In both male and female samples, combined power of discrimination exceeded 0.999999999. As confirmed by linkage disequilibrium test, significant association of marker pair DXS10074-DXS10079 (P = 0.0004) within LG2 and marker pair DXS10101-DXS10103 (P = 0.0003) within LG3 was found only in male samples. Number of observed haplotypes in our sample pool amounted 3.01, 7.53, 5 and 3.25% of the number of possible haplotypes for LG1, LG2, LG3 and LG4, respectively. According to haplotype diversity value of 0.9981, LG1 was the most informative. In comparison of south Croatia with 26 world populations, pair-wise \({{\text{F}}_{\text{ST}}}^{\text{*}}\) values increase in parallel with geographical distance. Overall statistical assessment confirmed suitability of Investigator® Argus X-12 kit for forensic casework in both identification and familial testing in the population of south Croatia.

Keywords

X-STR Investigator® Argus X-12 Population study South Croatia Forensics 

Notes

Acknowledgements

We are particularly grateful for having had the opportunity to collaborate with Branka Gršković, our late colleague, who had generously dedicated her time and great merit in making this research possible.

Funding

This study was funded by the Ministry of the Interior of Croatia.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11033_2017_4096_MOESM1_ESM.pdf (208 kb)
Supplementary material 1 (PDF 208 KB)
11033_2017_4096_MOESM2_ESM.xlsx (35 kb)
Supplementary material 2 (XLSX 35 KB)

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Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  • Gordan Mršić
    • 1
    • 2
  • Petar Ozretić
    • 3
  • Josip Crnjac
    • 4
  • Siniša Merkaš
    • 1
    • 2
  • Ivana Račić
    • 1
    • 2
  • Sara Rožić
    • 1
    • 2
  • Viktorija Sukser
    • 1
    • 2
  • Maja Popović
    • 5
  • Marina Korolija
    • 1
    • 2
  1. 1.Forensic Science Centre “Ivan Vučetić”ZagrebCroatia
  2. 2.Forensic Science OfficeUniversity of ZagrebZagrebCroatia
  3. 3.Ruđer Bošković InstituteZagrebCroatia
  4. 4.University Department for Forensic SciencesUniversity of SplitSplitCroatia
  5. 5.Faculty of Veterinary MedicineUniversity of ZagrebZagrebCroatia

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