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Molecular Biology Reports

, Volume 42, Issue 8, pp 1289–1293 | Cite as

Rs6922269 marker at the MTHFD1L gene predict cardiovascular mortality in males after acute coronary syndrome

  • J. A. Hubacek
  • V. Staněk
  • M. Gebauerová
  • R. Poledne
  • M. Aschermann
  • H. Skalická
  • J. Matoušková
  • A. Kruger
  • M. Pěnička
  • H. Hrabáková
  • J. Veselka
  • P. Hájek
  • V. Lánská
  • V. Adámková
  • J. Piťha
Article

Abstract

Myocardial infarction (MI) is the leading cause of death in industrialized countries. All the traditional risk factors for MI are responsible for approximately 50 % of cases of MI cases. Attention therefore has recently focused on genetic variants that are not associated with conventional risk factors. One of them is the marker rs6922269, which has been suggested as a risk factor for development of MI in Western populations. We analyzed the relationship between rs6922269 variant on MTHFD1L gene and (i) risk of the acute coronary syndrome (ACS) in the Czech population and (ii) mortality in 7 years follow up. Rs6922269 (G>A) variant was analyzed (CR 99.3 % for patients and 98.0 % for controls) by PCR–RFLP in consecutively examined 1614 men and 503 women with ACS (age below 65 years) and in population-based controls––1191 men and 1368 women (aged up to 65 years). ANOVA and Chi square were used for statistical analysis. The genotype frequencies were almost identical (P = 0.87) in the ACS patients and in controls and no differences were observed, if males (P = 0.73) and females (P = 0.93) were analysed separately. In addition, rs6922269 polymorphism was not associated with the classical risk factors (dyslipidemia, hypertension, obesity, smoking, diabetes) in control population. Cardiovascular mortality was significantly higher in males, carriers of the AA genotype (P < 0.001, OR 2.52, 95 % CI 1.40–4.55, for AA vs. +G). We conclude, that rs6922269 variant at MTHFD1L gene could be an important prognostic factor for cardiovascular mortality in patients after ACS.

Keywords

MTHFD1L Acute coronary syndrome Polymorphism Slavonic population 

Notes

Acknowledgments

This work was supported by project NT12217-5 (Internal Grant Agency, Ministry of Health, Czech Republic) and by the project (Ministry of Health, Czech Republic) for the development of research organisation 00023001 (IKEM, Prague, Czech Republic)––Institutional support.

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • J. A. Hubacek
    • 1
    • 9
  • V. Staněk
    • 2
  • M. Gebauerová
    • 2
  • R. Poledne
    • 1
  • M. Aschermann
    • 3
  • H. Skalická
    • 3
  • J. Matoušková
    • 4
  • A. Kruger
    • 4
  • M. Pěnička
    • 5
  • H. Hrabáková
    • 2
    • 5
  • J. Veselka
    • 6
  • P. Hájek
    • 6
  • V. Lánská
    • 7
  • V. Adámková
    • 8
  • J. Piťha
    • 1
  1. 1.Center for Experimental MedicineInstitute for Clinical and Experimental MedicinePragueCzech Republic
  2. 2.Cardiology DepartmentInstitute for Clinical and Experimental MedicinePragueCzech Republic
  3. 3.2nd Department of Internal MedicineGeneral University HospitalPragueCzech Republic
  4. 4.Department of CardiologyHomolka HospitalPragueCzech Republic
  5. 5.Department of Cardiology, CardiocenterUniversity Hospital Královské VinohradyPragueCzech Republic
  6. 6.Department of Cardiology, 2nd Medical School, University Hospital MotolCharles UniversityPragueCzech Republic
  7. 7.Statistical UnitInstitute for Clinical and Experimental MedicinePragueCzech Republic
  8. 8.Preventive Cardiology DepartmentInstitute for Clinical and Experimental MedicinePragueCzech Republic
  9. 9.IKEM-DEM-LMGPrague 4Czech Republic

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