Reduction of EPSP synthase in transgenic wild turnip (Brassica rapa) weed via suppression of aroA
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EPSPS is coded with the aroA gene, a key enzyme that catalyzes the penultimate step of shikimate pathway. The current study focuses on the suppression of aroA gene in weedy Brassica rapa. For this purpose B. rapa was transformed with double-stranded RNA interference construct designed to silence aroA gene. This developed in a significant decline in EPSPS (about 72 %) in T0 and T1 plants. In order to study the gene flow, the B. rapa control and B. napus plants were pollinated with T0 B. rapa. Results showed that in the next generation of challenging plants, the pollinated normal B. rapa showed the T1 symptoms and performance. Statistical analysis of data showed that knocking down of aroA will lead to a weakness and decreasing in investigated morphological, physiological and phonological characteristics. Meanwhile pollinated B. napus plant species have been not fertilized by T0 B. rapa. To conclude current result is the first evidence of aroA gene inhibition induces a high decrease in EPSPS protein in B. rapa. Also this result provides a basis for the future investigation in order to controlling B. rapa via molecular approach along with agronomical, biological and chemical methods regarding environmental considerations.
KeywordsWeed control Brassica rapa dsRNAi aroA suppression
Razi University has financially supported this research Project No. 996. I thank Dr. Noorbakhsh Hooti for critical reading of this manuscript and Dr. K. Yari (Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran) for technical supports. Thanks to Zagros Biotechnology Section of Razi University Incubator for technical Supports.
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