Reduction of EPSP synthase in transgenic wild turnip (Brassica rapa) weed via suppression of aroA
- 186 Downloads
EPSPS is coded with the aroA gene, a key enzyme that catalyzes the penultimate step of shikimate pathway. The current study focuses on the suppression of aroA gene in weedy Brassica rapa. For this purpose B. rapa was transformed with double-stranded RNA interference construct designed to silence aroA gene. This developed in a significant decline in EPSPS (about 72 %) in T0 and T1 plants. In order to study the gene flow, the B. rapa control and B. napus plants were pollinated with T0 B. rapa. Results showed that in the next generation of challenging plants, the pollinated normal B. rapa showed the T1 symptoms and performance. Statistical analysis of data showed that knocking down of aroA will lead to a weakness and decreasing in investigated morphological, physiological and phonological characteristics. Meanwhile pollinated B. napus plant species have been not fertilized by T0 B. rapa. To conclude current result is the first evidence of aroA gene inhibition induces a high decrease in EPSPS protein in B. rapa. Also this result provides a basis for the future investigation in order to controlling B. rapa via molecular approach along with agronomical, biological and chemical methods regarding environmental considerations.
KeywordsWeed control Brassica rapa dsRNAi aroA suppression
Razi University has financially supported this research Project No. 996. I thank Dr. Noorbakhsh Hooti for critical reading of this manuscript and Dr. K. Yari (Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran) for technical supports. Thanks to Zagros Biotechnology Section of Razi University Incubator for technical Supports.
- 1.Holm L, Doll J, Holm E, Pancho J, Herberger J (1997) World Weeds. Natural Histories and Distribution. John Wiley and Sons Inc, USAGoogle Scholar
- 3.Kahrizi D, Salmanian AH, Afshari A, Moieni A, Mousavi A (2007) Simultaneous substitution of Gly96 to Ala183 to Thr in 5-enolpyruvylshikimate 3-phosphate synthase gene of E. coli (k12) and transformation of rapeseed (Brassica napus L.) in order to make tolerance to glyphosate. Plant Cell Rep 26:95–104CrossRefPubMedGoogle Scholar
- 4.Kahrizi D, Salmanian AH (2008) Substitution of Ala183Thr in aroA Product of E. coli (k12) and Transformation of Rapeseed (Brassica napus) with Altered Gene to Tolerance of Plant to Roundup. Transgenic Plant J 2(2):170–175Google Scholar
- 19.Niu JH, Jian H, Xu JM, Guo YD, Liu Q (2010) RNAi technology extends its reach: engineering plant resistance against harmful eukaryotes. Afr J Biotechnol 9(45):7573–7582Google Scholar
- 20.Sambrook J, Russell DW (2001) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar
- 31.Budewig S, Léon J (2003) Higher yield stability for oilseed rape hybrids. In: 11th International Rapeseed Congress, Copenhagen, Denmark, 6–10 July 2003, pp 347–349Google Scholar
- 32.Han-Zhong W, Gui-Hua L, Xin-Fa W, Jing L, Qing YM, Wei H 2009 Heterosis and breeding of high oil content in rapeseed (Brassica napus L.). In: 16th Australian Research Assembly on Brassicas, Ballarat, VictoriaGoogle Scholar