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Identification and expression analysis of salt-responsive genes using a comparative microarray approach in Salix matsudana

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Abstract

Salt stress exerts negative effects on plant growth, development and yields, with roots being the primary site of both perception and damage. Salix matsudana (Chinese willow) is tolerant of high salinity. However, genes associated with this trait were rarely characterized. Therefore, we first performed salt-stress treatment on S. matsudana plants, then identified differentially expressed genes by comparison of salt-treated roots and untreated controls using microarray analysis. A total of 403 salt-responsive genes were identified, of which 239 were repressed and 164 were up-regulated. Functional classification analysis revealed that these genes belonged to families encoding proteins involved in metabolism, regulation of transcription, signal transduction, hormone responses, abiotic stress responses, and other processes related to growth and development. This suggested that when S. matsudana was confronted with salt stress, coordinated adjustments are made to physiological and biochemical processes, which would then allow more resources to be allocated to protective mechanisms to avoid salt injury. The expression patterns of representative genes were further validated and the diversity of the temporal profiles indicated that a combination of several genes and the initiation of diverse pathways performed functions in S. matsudana salt tolerance. This work represents the first study employing microarrays to investigate salt tolerance in S. matsudana. The data presented herein enhance our understanding of the molecular mechanisms of S. matsudana responses to salinity stress and lay the groundwork for genetic engineering strategies to improve stress tolerance of agronomically important species.

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Abbreviations

DEG:

Differentially expressed gene

qRT-PCR:

Quantitative real-time PCR

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Acknowledgments

We thank the anonymous referees and the editor for their comments and suggestions that helped improve the manuscript. We also thank Cuiyun Li and Huiqin Yang for their assistance in performing the experiments.

Funding

This work was supported by the State Key Development Program for Basic Research of China (Grant No. 2012CB114500-4), the National High Technology Research and Development Program of China (No. 2011AA100201), the National Natural Science Foundation of China (No. 31200465), and the Basic Scientific Research Project of Nonprofit Central Research Institutions (No. AFYBB2012040 and CAFYBB2011001).

The authors have declared that no competing interests exist.

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  1. State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Xiangshan Road, Beijing, 100091, China

    Mingying Liu, Guirong Qiao, Jing Jiang, Xiaojiao Han, Jian Sang & Renying Zhuo

  2. The Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Daqiao Road 73 Fuyang, Hangzhou, 311400, Zhejiang, China

    Mingying Liu, Guirong Qiao, Jing Jiang, Xiaojiao Han, Jian Sang & Renying Zhuo

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  1. Mingying Liu
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Correspondence to Renying Zhuo.

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Liu, M., Qiao, G., Jiang, J. et al. Identification and expression analysis of salt-responsive genes using a comparative microarray approach in Salix matsudana . Mol Biol Rep 41, 6555–6568 (2014). https://doi.org/10.1007/s11033-014-3539-1

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