Molecular Biology Reports

, Volume 41, Issue 9, pp 5923–5929 | Cite as

Application of SRAP markers in the identification of Stylosanthes guianensis hybrids

  • C. Q. Huang
  • G. D. Liu
  • C. J. Bai
  • W. Q. Wang
  • J. Tang


Sequence-related amplified polymorphism (SRAP) is a new molecular marker technology developed based on polymerase chain reaction. The authenticity of 84 progenies of 8 hybrid combinations of Stylosanthes guianensis was identified by SRAP markers to select the true hybrids used in the present study. A total of 35 SRAP primer combinations were selected from the parents of 8 hybrid combinations. The selected polymorphism primer combinations were applied to identify the authenticity of all progenies. The male parents of the primer combinations had specific markers, whereas the female parents did not. 68 progenies exhibited male parent-specific bands, which were identified as true hybrids. The rest of the progenies were considered self-hybrids because of the absence of male parent-specific bands. The results of hybrid identification provided solid evidence for further studies of hybrids and demonstrated SRAP molecular markers as a useful technology for assessing the purity of S. guianensis hybrids.


Stylosanthes guianensis Hybrid identification SRAP markers Male parent-specific markers 



The authors acknowledge the editors and anonymous reviewers for their valuable comments, all of which helped improve this manuscript. This work was supported by national nonprofit institute research grant of CATAS-TCGRI (No.1630032014028), the modern agro-industry technology research system (No.CARS-35), and science and technology support program found (No.2011BAD17B01-01-5).


  1. 1.
    Costa NMS, Ferreira MB (1984) Some Brazilian species of Stylosanthes. In: Stace HM, Edye LA (eds) The biology and agronomy of Stylosanthes. Academic Press, Sydney, pp 53–101Google Scholar
  2. 2.
    Williams RJ, Reid R, Schhult ze-Kraft R et al (1984) Natural distribution of Stylosanthes. In: S tace HM, Edye LA (eds) The biology and agronomy of Stylosanthes. Sydney, Academic Press, pp 73–101Google Scholar
  3. 3.
    Vieira MLC, Aguiar-perecin MLR, Martins PS (1993) A cytotaxonomic study in twelve Brazilian taxa of Stylosanthes Sw Leguminosae. Cytologia 58:305–311CrossRefGoogle Scholar
  4. 4.
    Burt RL, Miller CP (1975) Stylosanthes—a source of pasture legumes. Trop grasslands 9:117–123Google Scholar
  5. 5.
    Hwang MY, Wun LX, Zhang CZ (1986) The main varieties of forages and their evaluation in Southern China. In: proceedings of an international workshop, Cisarua, Indonesia, 19–23 August, 1985. p.202. (ACIAR Proceedings Series No.12)Google Scholar
  6. 6.
    Michalk DL, Fund NP Zhu CM (1993) Improvement of dry tropical rangelands in Hainan Island. Soc Range Manag 46:331–339CrossRefGoogle Scholar
  7. 7.
    Hu J, Quiros CF (1991) Identification of broccoli and cauliflower cultivars with RAPD markers. Plant Cell Rep 10:505–511CrossRefPubMedGoogle Scholar
  8. 8.
    Mongkolporn O, Dokmaihom Y, Kanchana-Udomkan C et al (2004) Genetic purity test of F1 hybrid Capsicum using molecular analysis. J Hortic Sci Biotechnol 79:449–451Google Scholar
  9. 9.
    Dongre A, Parkhi V (2005) Identification of cotton hybrid through the combination of PCR based RAPD, ISSR and microsatellite markers. J Plant Biochem Biotechnol 14:53–55CrossRefGoogle Scholar
  10. 10.
    Liu LW, Yan W, Gong YQ et al (2007) Assessment of genetic purity of tomato (Lycopersicon esculentum L.) hybrid using molecular markers. Sci Hortic-Amsterdam 115:7–12CrossRefGoogle Scholar
  11. 11.
    Garg A, Singh AK, Prabhu KV et al (2006) Utility of a fertility restorer gene linked marker for testing genetic purity of hybrid seeds in rice (Oryza sativa L.). Seed Sci Technol 34:9–18CrossRefGoogle Scholar
  12. 12.
    Li G, Quiros CF (2001) Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor Appl Genet 103:455–461CrossRefGoogle Scholar
  13. 13.
    Budak H, Shearman RC, Parmaksiz I et al (2004) Comparative analysis of seeded and vegetative biotype buffalograsses based on phylogenetic relationship using ISSRs, SSRs, RAPDs, and SRAPs. Theor Appl Genet 109:280–288CrossRefPubMedGoogle Scholar
  14. 14.
    Ferriol M, Picó B, Nuez F (2003) Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers. Theor Appl Genet 107:271–282CrossRefPubMedGoogle Scholar
  15. 15.
    Budak H, Shearman RC, Parmaksiz I (2004) Molecular characterization of buffalograss germplasm using sequence-related amplied polymorphism markers. Theor Appl Genet 108:328–334CrossRefPubMedGoogle Scholar
  16. 16.
    Budak H, Shearman RC, Gaussoin RE et al (2004) Application of sequence-related amplified polymorphism markers for characterization of turfgrass species. HortScience 39:955–958Google Scholar
  17. 17.
    Huang CQ, Huang DY, Zhang YF et al (2010) Genetic analysis for 57 accessions of Cynodon dactylon from 17 countries in 6 continents by SRAP markers. Trop grassl 44:274–281Google Scholar
  18. 18.
    Dongre AB, Raut MP, Bhandarkar MR et al (2011) Identification and genetic purity testing of cotton F1 hybrid using molecular markers. Indian J Biotechnol 10:301–306Google Scholar
  19. 19.
    Wu M, Jia X, Tian L et al (2010) Rapid and reliable purity identification of F1 hybrids of Maize (Zea may L.) using SSR markers. Maize Genomics Genet 1:1–4Google Scholar
  20. 20.
    Wang J, Zhong GY, Chin ECL et al (2002) Identification of parents of F1 hybrids through SSR profiling of maternal and hybrid tissue. Euphytica 124:29–34CrossRefGoogle Scholar
  21. 21.
    Akhare AA, Sakhare SB, Kulwal PL et al (2008) RAPD profile studies in Sorghum for identification of hybrids and their parents. Int J Integr Biol 3:18–24Google Scholar
  22. 22.
    Hulya I (2003) RAPD markers assisted varietal identification and genetic purity test in pepper, Capsicum annuum. Sci Hortic-Amsterdam 97:211–218CrossRefGoogle Scholar
  23. 23.
    Gomea SM, Denwar NN, Ramasubramanian T et al (2008) Identification of Peanut hybrids using microsatellite markers and horizontal polyacrylamide gel electrophoresis. Peanut Sci 35:123–129CrossRefGoogle Scholar
  24. 24.
    Dongre AB, Raut MP, Bhandarkar MR et al (2011) Identification and genetic purity testing of cotton F1 hybrid using molecular markers. Indian J Biotechnol 10:301–306Google Scholar
  25. 25.
    Lo Bianco C, Fernándea JA, Migliaro D et al (2011) Identification of F1 hybrids of artichoke by ISSR markers and morphological analysis. Mol Breed 27:157–170CrossRefGoogle Scholar
  26. 26.
    Lubell JD, Brand MH, Lehrer JM (2008) AFLP identification of Berberis thunbergii cultivars, inter-specific hybrids, and their parental species. J Hortic Sci Biotechnol 83:55–63Google Scholar
  27. 27.
    Xie XM, Zhou F, Zhang XQ et al (2009) Genetic variability and relationship between MT-1 elephant grass and closely related cultivars assessed by SRAP markers. J Genet 88:281–290CrossRefPubMedGoogle Scholar
  28. 28.
    van Treuren R, van Hintum ThJL (2009) Comparison of anonymous and targeted molecular markers for the estimation of genetic diversity in ex situ conserved Lactuca. Theor Appl Genet 119:1265–1279PubMedCentralCrossRefPubMedGoogle Scholar
  29. 29.
    Song ZQ, Li XF, Wang HG et al (2010) Genetic diversity and population structure of Salvia miltiorrhiza Bge in China revealed by ISSR and SRAP. Genetica 138:241–249CrossRefPubMedGoogle Scholar
  30. 30.
    Huang CQ, Zhang YF, Liu GD et al (2012) Genetic diversity of Cynodon Radiatus assessed by sequence-related amplified polymorphism markers. Biochem Syst Ecol 40:56–61CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern ChinaMinistry of AgricultureDanzhouChina

Personalised recommendations