Molecular Biology Reports

, Volume 41, Issue 8, pp 4919–4927 | Cite as

Cloning a glutathione peroxidase gene from Nelumbo nucifera and enhanced salt tolerance by overexpressing in rice

  • Ying Diao
  • Huaxue Xu
  • Guolin Li
  • Aiqing Yu
  • Xia Yu
  • Wanling Hu
  • Xingfei Zheng
  • Shaoqing Li
  • Youwei Wang
  • Zhongli Hu


A full-length cDNA clone encoding an 866 bp-length glutathione peroxidase protein (NnGPX) was isolated from lotus (Nelumbo nucifera L.). The deduced amino acid sequence of the NnGPX gene had significant homology with ATGPX6. A 3D structural model of the NnGPX was constructed by homology modeling. The cloned NnGPX gene was expressed in Escherichia coli, and a fusion protein of about 40 kDa was detected after isopropyl thiogalactoside induction. Under different concentrations of Na2SeO3 treatments, NnGPX was found to be an enzyme that does not contain selenium. Real-time PCR analysis showed that the NnGPX gene was expressed in all organs of lotus, and its high expression mainly occurred in organs with active metabolisms. NnGPX transcript increased remarkably in response to cold, heat, mechanical damage, and salt treatment. Subsequently, the NnGPX gene was introduced in Oryza sativa cv. Yuetai B. PCR results verified the integration of this gene into the genome of rice and reverse transcription-PCR verified that this gene had been expressed in transgenic rice. The transgenic plants were significantly more tolerant to salt stress compared with the wild-type.


Nelumbo nucifera Glutathione peroxidase (GPX) Reactive oxygen species (ROS) Rice Transformation Salt stress 



This research was supported by National Key Technologies R&D Program (No. 2012BAD27B01). The authors thank the anonymous referees for their critical comments that contributed toward improving this manuscript.


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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Ying Diao
    • 1
    • 2
  • Huaxue Xu
    • 1
  • Guolin Li
    • 1
  • Aiqing Yu
    • 1
  • Xia Yu
    • 1
  • Wanling Hu
    • 3
  • Xingfei Zheng
    • 1
  • Shaoqing Li
    • 1
  • Youwei Wang
    • 2
  • Zhongli Hu
    • 1
  1. 1.State Key Laboratory of Hybrid Rice, College of Life SciencesWuhan UniversityWuhanPeople’s Republic of China
  2. 2.School of Pharmaceutical SciencesWuhan UniversityWuhanPeople’s Republic of China
  3. 3.Institute of WTOWuhan UniversityWuhanPeople’s Republic of China

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