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Molecular Biology Reports

, Volume 41, Issue 7, pp 4601–4611 | Cite as

Molecular cloning and expression analysis of mannose receptor in blunt snout bream (Megalobrama amblycephala)

  • Xiaoling Liu
  • Xiaocheng Tang
  • Li Wang
  • Jie Li
  • Hong Wang
  • Shun Wei
  • Rui Fang
  • Wei Ji
  • Gailing Yuan
  • Nan Chen
  • Zemao Gu
  • Xueqin Liu
  • Weimin Wang
  • Muhammad Asim
  • Yang Zhou
  • Li Lin
Article

Abstract

Mannose receptor (MR) plays a significant role in innate immune responses to pathogens in vertebrates. Here we characterized the first teleost MR from Megalobrama amblycephala, named maMR and its expression patterns were investigated. The full-length maMR consists of 5,295 bp encoding a putative protein of 1,433 amino acids. The predicted amino acid sequences showed that maMR contained a signal peptide, a cysteine-rich domain, a single fibronectin type II domain, eight tandemly arranged C-type lectin-like domains, a transmembrane domain and a C-terminal cytoplasmic domain. Phylogenetic analysis revealed the highest similarity of maMR with Danio rerio MR predicted by computational analysis. The maMR-mRNAs were ubiquitously transcribed in different tissues, However the highest transcripts were observed in head kidney. Transcripts of maMR significantly increased at the late stages of embryo and continued to be at the high levels after hatching. The maMR transcripts were significantly increased in M. amblycephala after stimulation with killed Aeromonas hydrophila.

Keywords

Mannose receptor Megalobrama amblycephala Cloning Expression 

Abbreviations

CR

Cysteine-rich domain

CTLDs

C-type lectin-like domains

FNII

Fibronectin type II domain

MR

Mannose receptor

PRRs

Pattern-recognition receptors

RACE

Rapid amplification of cDNA ends techniques

RT-PCR

Reverse transcription polymerase chain reaction

qRT-PCR

Quantitative real time polymerase chain reaction

Notes

Acknowledgments

This work was jointly supported by the National Basic Research Program of China (973 Program, No. 2009CB118706), the Fundamental Research Funds for the Central Universities (52204-12020, 2013PY069, 2013PY70, 2013 PY071, No. 2011QC029, 2011PY023, 2011PY043, 2011SC27, 2011ZC011, 2012YB08) and Natural Science Foundation of Hubei Province of China (2009CDB424).

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Xiaoling Liu
    • 1
  • Xiaocheng Tang
    • 1
  • Li Wang
    • 1
  • Jie Li
    • 1
  • Hong Wang
    • 1
  • Shun Wei
    • 1
  • Rui Fang
    • 1
  • Wei Ji
    • 1
  • Gailing Yuan
    • 1
  • Nan Chen
    • 1
  • Zemao Gu
    • 1
  • Xueqin Liu
    • 1
  • Weimin Wang
    • 2
    • 3
  • Muhammad Asim
    • 1
  • Yang Zhou
    • 1
  • Li Lin
    • 1
    • 2
    • 3
    • 4
  1. 1.Department of Aquatic Animal Medicine, College of FisheriesHuazhong Agricultural UniversityWuhanPeople’s Republic of China
  2. 2.Hubei Collaborative Innovation Center for Freshwater AquacultureWuhanPeople’s Republic of China
  3. 3.Key Lab of Freshwater Animal BreedingMinistry of AgricultureWuhanPeople’s Republic of China
  4. 4.State Key Laboratory of Agricultural MicrobiologyWuhanPeople’s Republic of China

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