Molecular Biology Reports

, Volume 41, Issue 7, pp 4493–4505 | Cite as

Long non-coding RNAs: new players in ocular neovascularization

  • Xue-Dong Xu
  • Ke-Ran Li
  • Xiu-Miao Li
  • Jin Yao
  • Jiang Qin
  • Biao Yan


Pathological neovascularization are the most prevalent causes of moderate or severe vision loss. Long non-coding RNAs (lncRNAs) have emerged as a novel class of regulatory molecules involved in numerous biological processes and complicated diseases. However, the role of lncRNAs in ocular neovascularization is still unclear. Here, we constructed a murine model of ocular neovascularization, and determined lncRNA expression profiles using microarray analysis. We identified 326 or 51 lncRNAs that were significantly either up-regulated or down-regulated in the vaso-obliteration or neovascularization phase, respectively. Based on Pearson correlation analysis, lncRNAs/mRNAs co-expression networks were constructed. GO enrichment analysis of lncRNAs-co-expressed mRNAs indicated that the biological modules were correlated with chromosome organization, extracellular region and guanylate cyclase activator activity in the vaso-obliteration phase, and correlated with cell proliferation, extracellular region and guanylate cyclase regulator activity in the neovascularization phase. KEGG pathway analysis indicated that MAPK signaling was the most significantly enriched pathway in both phases. Importantly, Vax2os1 and Vax2os2 were not only dynamically expressed in the vaso-obliteration and neovascularization phases, but also significantly altered in the aqueous humor of patients with neovascular age-related macular degeneration (AMD), suggesting a potential role of lncRNAs in the regulation of ocular neovascularization. Taken together, this study provided novel insights into the molecular pathogenesis of ocular neovascularization. The intervention of dysregulated lncRNA could become a potential target for the prevention and treatment of ocular vascular diseases.


Long non-coding RNA Ocular neovascularization Microarray analysis Age-related macular degeneration 



This work was generously supported by grants from the National Natural Science Foundation of China (Grant No. 81300241 to B.Y. and Grant No. 81371055 to Q.J.), grants from the National clinical key construction project [Grant No. (2012) 649 to Q.J.], and grants from the Medical Science and Technology Development Project Fund of Nanjing (Grant No. ZKX 12047 to Q.J., Grant No. YKK12207 to G.F.-C., and Grant No. YKK12208 to J.Y.).

Supplementary material

11033_2014_3320_MOESM1_ESM.xls (90 kb)
Table S1: Differentially expressed lncRNAs between P12 and P7 experimental group (XLS 90 kb)
11033_2014_3320_MOESM2_ESM.xls (30 kb)
Table S2: Differentially expressed lncRNAs between P17 and P12 experimental group (XLS 30 kb)
11033_2014_3320_MOESM3_ESM.xls (42 kb)
Table S3: Differentially expressed lncRNAs between P17 and P7 experimental group (XLS 42 kb)
11033_2014_3320_MOESM4_ESM.xls (16 kb)
Table S4: Common differentially expressed lncRNAs among the three experimental groups (XLS 16 kb)
11033_2014_3320_MOESM5_ESM.xls (67 kb)
Table S5: Correlation analysis between the differential expressed lncRNAs and mRNAs (P12 vs. P7) (XLS 67 kb)
11033_2014_3320_MOESM6_ESM.xls (58 kb)
Table S6: Correlation analysis between the differential expressed lncRNAs and mRNAs (P17 vs. P12) (XLS 57 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.Eye HospitalNanjing Medical UniversityNanjingChina

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