Molecular Biology Reports

, Volume 41, Issue 4, pp 2299–2306 | Cite as

hOGG1 Ser326Cys polymorphism and lung cancer susceptibility: a meta-analysis



The Ser326Cys polymorphism in the human 8-oxogunaine glycosylase (hOGG1) gene with lung cancer susceptibility had been investigated by the approaches of PCR–RFLP, PCR–SSCP and ASA. Due to limited specimen and different approaches the conclusion was drawn toughly. To evaluate this correlation comprehensively, a meta-analysis was performed based on 30 case–control studies, including 10,327 cases and 12,148 controls. The random-effects model was used to estimate the odds ratios and 95 % confidence interval for various contrasts of this polymorphism. The combined results suggested that the hOGG1 Ser326Cys polymorphism was not associated with lung cancer susceptibility in different genetic models. Similarly, in the stratified analyses by ethnicity and source of control, no risk was observed between all the genetic models and lung cancer risk. Our meta-analysis revealed that there was little correlation between the hOGG1 Ser326Cys polymorphism and the risk of lung cancer.


Ser326Cys hOGG1 Polymorphism Susceptibility Lung cancer 



This project was supported by National High Technology Research and Development Program in China (2011AA02A110).


  1. 1.
    Li Z, Guan W, Li MX, Zhong ZY, Qian CY, Yang XQ, Liao L, Li ZP, Wang D (2011) Genetic polymorphism of DNA base-excision repair genes (APE1, OGG1 and XRCC1) and their correlation with risk of lung cancer in a Chinese population. Arch Med Res 42(3):226–234PubMedCrossRefGoogle Scholar
  2. 2.
    Aburatani H, Hippo Y, Ishida T, Takashima R, Matsuba C, Kodama T, Takao M, Yasui A, Yamamoto K, Asano M (1997) Cloning and characterization of mammalian 8-hydroxyguanine-specific DNA glycosylase/apurinic, apyrimidinic lyase, a functional mutM homologue. Cancer Res 57(11):2151–2156PubMedGoogle Scholar
  3. 3.
    Arai K, Morishita K, Shinmura K, Kohno T, Kim SR, Nohmi T, Taniwaki M, Ohwada S, Yokota J (1997) Cloning of a human homolog of the yeast OGG1 gene that is involved in the repair of oxidative DNA damage. Oncogene 14(23):2857–2861PubMedCrossRefGoogle Scholar
  4. 4.
    Lu R, Nash HM, Verdine GL (1997) A mammalian DNA repair enzyme that excises oxidatively damaged guanines maps to a locus frequently lost in lung cancer. Curr Biol 7(6):397–407PubMedCrossRefGoogle Scholar
  5. 5.
    Radicella JP, Dherin C, Desmaze C, Fox MS, Boiteux S (1997) Cloning and characterization of hOGG1, a human homolog of the OGG1 gene of Saccharomyces cerevisiae. Proc Natl Acad Sci USA 94(15):8010–8015PubMedCentralPubMedCrossRefGoogle Scholar
  6. 6.
    Roldán-Arjona T, Wei YF, Carter KC, Klungland A, Anselmino C, Wang RP, Augustus M, Lindahl T (1997) Molecular cloning and functional expression of a human cDNA encoding the antimutator enzyme 8-hydroxyguanine-DNA glycosylase. Proc Natl Acad Sci USA 94(15):8016–8020PubMedCentralPubMedCrossRefGoogle Scholar
  7. 7.
    Rosenquist TA, Zharkov DO, Grollman AP (1997) Cloning and characterization of a mammalian 8-oxoguanine DNA glycosylase. Proc Natl Acad Sci USA 94(14):7429–7434PubMedCentralPubMedCrossRefGoogle Scholar
  8. 8.
    Wang YJ, Ho YS, Lo MJ, Lin JK (1995) Oxidative modification of DNA bases in rat liver and lung during chemical carcinogenesis and aging. Chem Biol Interact 94(2):135–145PubMedCrossRefGoogle Scholar
  9. 9.
    Nakae D, Kobayashi Y, Akai H, Andoh N, Satoh H, Ohashi K, Tsutsumi M, Konishi Y (1997) Involvement of 8-hydroxyguanine formation in the initiation of rat liver carcinogenesis by low dose levels of N-nitrosodiethylamine. Cancer Res 57(7):1281–1287PubMedGoogle Scholar
  10. 10.
    Kohno T, Shinmura K, Tosaka M, Tani M, Kim SR, Sugimura H, Nohmi T, Kasai H, Yokota J (1998) Genetic polymorphisms and alternative splicing of the hOGG1 gene, that is involved in the repair of 8-hydroxyguanine in damaged DNA. Oncogene 16(25):3219–3225PubMedCrossRefGoogle Scholar
  11. 11.
    Shinmura K, Kasai H, Sasaki A, Sugimura H, Yokota J (1997) 8-Hydroxyguanine (7,8-dihydro-8-oxoguanine) DNA glycosylase and AP lyase activities of hOGG1 protein and their substrate specificity. Mutat Res 385(1):75–82PubMedCrossRefGoogle Scholar
  12. 12.
    Ishida T, Takashima R, Fukayama M, Hamada C, Hippo Y, Fujii T, Moriyama S, Matsuba C, Nakahori Y, Morita H, Yazaki Y, Kodama T, Nishimura S, Aburatani H (1999) New DNA polymorphisms of human MMH/OGG1 gene: prevalence of one polymorphism among lung-adenocarcinoma patients in Japanese. Int J Cancer 80(1):18–21PubMedCrossRefGoogle Scholar
  13. 13.
    Egger M, Davey Smith G, Schneider M, Minder C (1997) Bias in meta-analysis detected by a simple, graphical test. BMJ 315(7109):629–634PubMedCentralPubMedCrossRefGoogle Scholar
  14. 14.
    Le Marchand L, Donlon T, Lum-Jones A, Seifried A, Wilkens LR (2002) Association of the hOGG1 Ser326Cys polymorphism with lung cancer risk. Cancer Epidemiol Biomarkers Prev 11(4):409–412PubMedGoogle Scholar
  15. 15.
    Lan Q, Mumford JL, Shen M, Demarini DM, Bonner MR, He X, Yeager M, Welch R, Chanock S, Tian L, Chapman RS, Zheng T, Keohavong P, Caporaso N, Rothman N (2004) Oxidative damage-related genes AKR1C3 and OGG1 modulate risks for lung cancer due to exposure to PAH-rich coal combustion emissions. Carcinogenesis 25(11):2177–2181PubMedCrossRefGoogle Scholar
  16. 16.
    Vogel U, Nexø BA, Wallin H, Overvad K, Tjønneland A, Raaschou-Nielsen O (2004) No association between base excision repair gene polymorphisms and risk of lung cancer. Biochem Genet 42(11–12):453–460PubMedCrossRefGoogle Scholar
  17. 17.
    Loft S, Svoboda P, Kasai H, Tjønneland A, Vogel U, Møller P, Overvad K, Raaschou-Nielsen O (2006) Prospective study of 8-oxo-7,8-dihydro-2′-deoxyguanosine excretion and the risk of lung cancer. Carcinogenesis 27(6):1245–1250PubMedCrossRefGoogle Scholar
  18. 18.
    Matullo G, Dunning AM, Guarrera S, Baynes C, Polidoro S, Garte S, Autrup H, Malaveille C, Peluso M, Airoldi L, Veglia F, Gormally E, Hoek G, Krzyzanowski M, Overvad K, Raaschou-Nielsen O, Clavel-Chapelon F, Linseisen J, Boeing H, Trichopoulou A, Palli D, Krogh V, Tumino R, Panico S, Bueno-De-Mesquita HB, Peeters PH, Lund E, Pera G, Martinez C, Dorronsoro M, Barricarte A, Tormo MJ, Quiros JR, Day NE, Key TJ, Saracci R, Kaaks R, Riboli E, Vineis P (2006) DNA repair polymorphisms and cancer risk in non-smokers in a cohort study. Carcinogenesis 27(5):997–1007PubMedCrossRefGoogle Scholar
  19. 19.
    Sørensen M, Raaschou-Nielsen O, Hansen RD, Tjønneland A, Overvad K, Vogel U (2006) Interactions between the OGG1 Ser326Cys polymorphism and intake of fruit and vegetables in relation to lung cancer. Free Radic Res 40(8):885–891PubMedCrossRefGoogle Scholar
  20. 20.
    Zienolddiny S, Campa D, Lind H, Ryberg D, Skaug V, Stangeland L, Phillips DH, Canzian F, Haugen A (2006) Polymorphisms of DNA repair genes and risk of non-small cell lung cancer. Carcinogenesis 27(3):560–567PubMedCrossRefGoogle Scholar
  21. 21.
    Hatt L, Loft S, Risom L, Møller P, Sørensen M, Raaschou-Nielsen O, Overvad K, Tjønneland A, Vogel U (2008) OGG1 expression and OGG1 Ser326Cys polymorphism and risk of lung cancer in a prospective study. Mutat Res 639(1–2):45–54PubMedCrossRefGoogle Scholar
  22. 22.
    Karahalil B, Emerce E, Koçer B, Han S, Alkiş N, Karakaya AE (2008) The association of OGG1 Ser326Cys polymorphism and urinary 8-OHdG levels with lung cancer susceptibility: a hospital-based case–control study in Turkey. Arh Hig Rada Toksikol 59(4):241–250PubMedCrossRefGoogle Scholar
  23. 23.
    Okasaka T, Matsuo K, Suzuki T, Ito H, Hosono S, Kawase T, Watanabe M, Yatabe Y, Hida T, Mitsudomi T, Tanaka H, Yokoi K, Tajima K (2009) hOGG1 Ser326Cys polymorphism and risk of lung cancer by histological type. J Hum Genet 54(12):739–745PubMedCrossRefGoogle Scholar
  24. 24.
    Qian Q, Liu R, Lei Z, You J, Zhou Q, Zhang HT (2011) Meta analysis of association between Ser326Cys polymorphism of hOGG1 gene and risk of lung cancer. Zhongguo Fei Ai Za Zhi 14(3):205–210PubMedGoogle Scholar
  25. 25.
    Sugimura H, Kohno T, Wakai K, Nagura K, Genka K, Igarashi H, Morris BJ, Baba S, Ohno Y, Gao C, Li Z, Wang J, Takezaki T, Tajima K, Varga T, Sawaguchi T, Lum JK, Martinson JJ, Tsugane S, Iwamasa T, Shinmura K, Yokota J (1999) hOGG1 Ser326Cys polymorphism and lung cancer susceptibility. Cancer Epidemiol Biomarkers Prev 8(8):669–674PubMedGoogle Scholar
  26. 26.
    Wikman H, Risch A, Klimek F, Schmezer P, Spiegelhalder B, Dienemann H, Kayser K, Schulz V, Drings P, Bartsch H (2000) hOGG1 polymorphism and loss of heterozygosity (LOH): significance for lung cancer susceptibility in a caucasian population. Int J Cancer 88(6):932–937PubMedCrossRefGoogle Scholar
  27. 27.
    Ito H, Hamajima N, Takezaki T, Matsuo K, Tajima K, Hatooka S, Mitsudomi T, Suyama M, Sato S, Ueda R (2002) A limited association of OGG1 Ser326Cys polymorphism for adenocarcinoma of the lung. J Epidemiol 12(3):258–265PubMedCrossRefGoogle Scholar
  28. 28.
    Sunaga N, Kohno T, Yanagitani N, Sugimura H, Kunitoh H, Tamura T, Takei Y, Tsuchiya S, Saito R, Yokota J (2002) Contribution of the NQO1 and GSTT1 polymorphisms to lung adenocarcinoma susceptibility. Cancer Epidemiol Biomarkers Prev 11(8):730–738PubMedGoogle Scholar
  29. 29.
    Park J, Chen L, Tockman MS, Elahi A, Lazarus P (2004) The human 8-oxoguanine DNA N-glycosylase 1 (hOGG1) DNA repair enzyme and its association with lung cancer risk. Pharmacogenetics 14(2):103–109PubMedCrossRefGoogle Scholar
  30. 30.
    Hung RJ, Brennan P, Canzian F, Szeszenia-Dabrowska N, Zaridze D, Lissowska J, Rudnai P, Fabianova E, Mates D, Foretova L, Janout V, Bencko V, Chabrier A, Borel S, Hall J, Boffetta P (2005) Large-scale investigation of base excision repair genetic polymorphisms and lung cancer risk in a multicenter study. J Natl Cancer Inst 97(8):567–576PubMedCrossRefGoogle Scholar
  31. 31.
    Liang G, Pu Y, Yin L (2005) Rapid detection of single nucleotide polymorphisms related with lung cancer susceptibility of Chinese population. Cancer Lett 223(2):265–274PubMedCrossRefGoogle Scholar
  32. 32.
    Kohno T, Kunitoh H, Toyama K, Yamamoto S, Kuchiba A, Saito D, Yanagitani N, Ishihara S, Saito R, Yokota J (2006) Association of the OGG1–Ser326Cys polymorphism with lung adenocarcinoma risk. Cancer Sci 97(8):724–728PubMedCrossRefGoogle Scholar
  33. 33.
    Chang CH, Hsiao CF, Chang GC, Tsai YH, Chen YM, Huang MS, Su WC, Hsieh WS, Yang PC, Chen CJ, Hsiung CA (2009) Interactive effect of cigarette smoking with human 8-oxoguanine DNA N-glycosylase 1 (hOGG1) polymorphisms on the risk of lung cancer: a case–control study in Taiwan. Am J Epidemiol 170(6):695–702PubMedCrossRefGoogle Scholar
  34. 34.
    Miyaishi A, Osawa K, Osawa Y, Inoue N, Yoshida K, Kasahara M, Tsutou A, Tabuchi Y, Sakamoto K, Tsubota N, Takahashi J (2009) MUTYH Gln324His gene polymorphism and genetic susceptibility for lung cancer in a Japanese population. J Exp Clin Cancer Res 28:10PubMedCentralPubMedCrossRefGoogle Scholar
  35. 35.
    Liu CJ, Hsia TC, Tsai RY, Sun SS, Wang CH, Lin CC, Tsai CW, Huang CY, Hsu CM, Bau DT (2010) The joint effect of hOGG1 single nucleotide polymorphism and smoking habit on lung cancer in Taiwan. Anticancer Res 30(10):4141–4145PubMedGoogle Scholar
  36. 36.
    Janik J, Swoboda M, Janowska B, Cieśla JM, Gackowski D, Kowalewski J, Olinski R, Tudek B, Speina E (2011) 8-Oxoguanine incision activity is impaired in lung tissues of NSCLC patients with the polymorphism of OGG1 and XRCC1 genes. Mutat Res 709–710:21–31PubMedCrossRefGoogle Scholar
  37. 37.
    Kohno T, Kunitoh H, Mimaki S, Shiraishi K, Kuchiba A, Yamamoto S, Yokota J (2011) Contribution of the TP53, OGG1, CHRNA3, and HLA-DQA1 genes to the risk for lung squamous cell carcinoma. J Thorac Oncol 6(4):813–817PubMedCrossRefGoogle Scholar
  38. 38.
    Guan P, Huang D, Yin Z, Zhou B (2011) Association of the hOGG1 Ser326Cys polymorphism with increased lung cancer susceptibility in Asians: a meta-analysis of 18 studies including 7592 cases and 8129 controls. Asian Pac J Cancer Prev 12(4):1067–1072PubMedGoogle Scholar
  39. 39.
    Li H, Hao X, Zhang W, Wei Q, Chen K (2008) The hOGG1 Ser326Cys polymorphism and lung cancer risk: a meta-analysis. Cancer Epidemiol Biomarkers Prev 17(7):1739–1745PubMedCrossRefGoogle Scholar
  40. 40.
    Gao, F (2009) The study of the relationship between the polymorphism of h OGG1 genes and XPD genes and susceptibility to lung cancer. Master degree thesis, Kunming Medical College, China. (In Chinese) Google Scholar
  41. 41.
    Wang W et al (2005) Genetic polymorphism in Hoggle and susceptibility to lung cancer. Carcinog Teratogenesis Mutagen 17:101–103Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.The Key LabCancer Center, Chinese PLA General HospitalBeijingPeople’s Republic of China
  2. 2.Department of OncologyPLA No. 161 Center HospitalWuhanPeople’s Republic of China
  3. 3.The College of Life Sciences and Bio-EngineeringBeijing Jiao-Tong UniversityBeijingPeople’s Republic of China

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