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Molecular Biology Reports

, Volume 41, Issue 4, pp 2229–2241 | Cite as

Differentially expressed genes in response to gamma-irradiation during the vegetative stage in Arabidopsis thaliana

  • Jin-Baek Kim
  • Sang Hoon Kim
  • Bo-Keun Ha
  • Si-Yong Kang
  • Cheol Seong Jang
  • Yong Weon Seo
  • Dong Sub Kim
Article

Abstract

Biochemical and physiological processes in plants are affected by gamma-irradiation, which causes significant changes in gene transcripts and expression. To identify the differentially expressed Arabidopsis genes in response to gamma-irradiation, we performed a microarray analysis with rosette leaves during the vegetative stage. Arabidopsis plants were exposed to a wide spectrum doses of gamma ray (100, 200, 300, 400, 800, 1200, 1600 or 2000 Gy) for 24 h. At the dose range from 100 to 400 Gy, irradiated plants were found to be shorter than controls after 8 days of irradiation, while doses over 800 Gy caused severe growth retardation. Therefore, 100 and 800 Gy were selected as adequate doses for microarray analysis to identify differentially expressed genes. Among the 20,993 genes used as microarray probes, a total number of 496 and 1,042 genes were up-regulated and down-regulated by gamma-irradiation, respectively (P < 0.05). We identified the characteristics of the genes that were up-and down-regulated fourfold higher genes by gamma irradiation according to The arabidopsis information resource gene ontology. To confirm the microarray results, we performed a northern blot and quantitative real-time PCR with several selected genes that had a large difference in expression after irradiation. In particular, genes associated with lipid transfer proteins, histones and transposons were down-regulated by 100 and/or 800 Gy of gamma irradiation. The expression patterns of selected genes were generally in agreement with the microarray results, although there were quantitative differences in the expression levels.

Keywords

Arabidopsis thaliana Gamma-irradiation Microarray analysis Northern blot Quantitative real-time PCR 

Abbreviations

TAIR

The Arabidopsis Information Resource

GO

Gene Ontology

qRT-PCR

Quantitative real-time PCR

ROS

Reactive oxygen species

SSB

Single-strand breakage

DSB

Double-strand breakage

AP

Apurinic/apyrimidinic

DAS

Days after seeding

DAI

Days after irradiation

LTP

Lipid transfer protein

Notes

Acknowledgments

This work was supported by grants from the Korea Science and Engineering foundation (KOSEF) in the Ministry of Science, ICT and Future Planning (MSIP) and the Korea Atomic Energy Research Institute (KAERI).

Supplementary material

11033_2014_3074_MOESM1_ESM.pdf (281 kb)
Supplementary material 1 (PDF 281 kb)
11033_2014_3074_MOESM2_ESM.pdf (86 kb)
Supplementary material 2 (PDF 85 kb)
11033_2014_3074_MOESM3_ESM.pdf (29 kb)
Supplementary material 3 (PDF 29 kb)
11033_2014_3074_MOESM4_ESM.pdf (288 kb)
Supplementary material 4 (PDF 287 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Jin-Baek Kim
    • 1
    • 3
  • Sang Hoon Kim
    • 1
  • Bo-Keun Ha
    • 1
  • Si-Yong Kang
    • 1
  • Cheol Seong Jang
    • 2
  • Yong Weon Seo
    • 3
  • Dong Sub Kim
    • 1
  1. 1.Advanced Radiation Technology InstituteKorea Atomic Energy Research InstituteJeongeupRepublic of Korea
  2. 2.Department of Applied Plant Sciences TechnologyKangwon National UniversityChuncheonRepublic of Korea
  3. 3.Division of BiotechnologyKorea UniversitySeoulRepublic of Korea

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