Glycine betaine protects tomato (Solanum lycopersicum) plants at low temperature by inducing fatty acid desaturase7 and lipoxygenase gene expression
- 1.2k Downloads
Cold stress is among the environmental stressors limiting productivity, yield and quality of agricultural plants. Tolerance to cold stress is associated with the increased unsaturated fatty acids ratio in the plant membranes which are also known to be substrates of octadecanoid pathway for jasmonate and other oxylipins biosynthesis. Accumulation of osmoprotectant, glycine betaine (GB) is well known to be effective in the protecting membranes and mitigating cold stress effects but, the mode of action is poorly understood. We studied the role of GB in cold stress responses of two tomato cultivated varieties; Gerry (cold stress sensitive) and T47657 (moderately cold stress tolerant) and compared the differences in lypoxygenase-13 (TomLOXF) and fatty acid desaturase 7 (FAD7) gene expression profiles and physiological parameters including relative growth rates, relative water content, osmotic potential, photosynthetic efficiency, membrane leakage, lipid peroxidation levels. Our results indicated that GB might have a role in inducing FAD7 and LOX expressions for providing protection against cold stress in tomato plants which could be related to the desaturation process of lipids leading to increased membrane stability and/or induction of other genes related to stress defense mechanisms via octadecanoid pathway or lipid peroxidation products.
KeywordsTomato Chilling stress FAD7 LOX Lipid peroxidation PUFAs
This research was supported by Ege University Research Foundation with the grant number 2010-FEN-021. The authors are grateful to Tuna Saygan from Syngenta-Turkey for providing us Gerry and T47657 seeds and to Kera Yücel and Levent Soylu for their technical assistance.
- 11.Dave A, Graham A (2012) Oxylipin signaling: a distinct role for the jasmonic acid precursor cis-(+)-12-Oxo-phytodienoic acid (cis-OPDA). Front Plant Sci 3(42):1–6Google Scholar
- 18.Li C, Liu G, Xu C, Lee GI, Bauer P, Ling HQ, Gana MW, Howe GA (2003) The tomato suppressor of prosystemin-mediated responses2 gene encodes a fatty acid desaturase required for the biosynthesis of jasmonic acid and the production of a systemic wound signal for defense gene expression. Plant Cell 15:1646–1661PubMedCentralPubMedCrossRefGoogle Scholar
- 34.Ariizumi T, Kishitani S, Inatsugi R, Nishida I, Murata N, Toriyama K (2002) An increase in unsaturation of fatty acids in phosphatidylglycerol from leaves improves the rates of photosynthesis and growth at low temperatures in transgenic rice seedlings. Plant Cell Physiol 43:751–758PubMedCrossRefGoogle Scholar
- 35.Kaur G, Kumar S, Nayyar H, Upadhyaya HD (2008) Cold stress injury during the pod filling phase in chickpea (Cicer arietinum L.): effects on quantitative and qualitative components of seeds. J Agric Crop Sci 194:457–464Google Scholar
- 43.Marocco A, Lorenzoni C, Fracheboud Y (2005) Chilling stress in maize. Maydica 50:571–580Google Scholar
- 50.Holley SR, Yalamanchili RD, Moura DS, Ryan CA, Stratmann JW (2003) Convergence of signalling pathways induced by systemin, oligosaccharide elicitors and ultraviolet B radiation at the level of MAPKs in Lycopersicon peruvianum suspension cultured cells. Plant Phyisol 132:1728–1732CrossRefGoogle Scholar