Differential gene expression profile in Pseudomonas putida NBRIC19-treated wheat (Triticum aestivum) plants subjected to biotic stress of Parthenium hysterophorus
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The inoculation of Pseudomonas putida NBRIC19 protected wheat plant from phytotoxic effect of Parthenium hysterophorus (Parthenium) and enhanced root length, shoot length, dry weight, spike length and chlorophyll content. With the aim to screen for genes differentially expressed in P. putida NBRIC19-inoculated wheat grown along with Parthenium (WPT), the suppression subtractive hybridization (SSH) methodology was employed. The SSH analysis was performed with WPC (uninoculated wheat grown along with Parthenium) as driver and WPT as tester. The cDNA library, enriched with differentially expressed ESTs (expressed sequence tags), were constructed from WPT. Following an initial screen of 165 ESTs in our library, 32 ESTs were identified, annotated and further validated by semiquantitative RT-PCR. The differentially expressed ESTs were associated with general stress response, defense response, growth and development, metabolic process, photosynthesis, signal transduction, and some other with unknown function. Five ESTs showing downregulation in expression level in response to Parthenium got upregulated due to P. putida NBRIC19 inoculation and further validated by quantitative real time PCR analysis at different time intervals viz. 15, 30, 45 and 90 days. SSH has been implemented for the first time to gain insights into molecular events underlying successful role of P. putida NBRIC19 in providing protection to wheat against Parthenium. The information generated in this study provides new clues to aid the understanding of genes corresponding to differentially expressed ESTs putatively involved in allelopathic interactions. Further characterization and functional analysis of these genes may provide valuable information for future studies of the molecular mechanism by which plants adapt to allelopathic effect of Parthenium.
KeywordsParthenium Suppression subtractive hybridization Pseudomonas putida Wheat Gene expression Real time PCR
The study was supported by Task Force Grant NWP-006 from Council of Scientific and Industrial Research (CSIR), New Delhi, India. Part of the work was supported by TATA Innovation Fellowship awarded to Dr. Chandra Shekhar Nautiyal by Department of Biotechnology, Government of India. Sandhya Mishra would like to thank CSIR for awarding Senior Research Fellowship.
- 5.Saranga Y, Paterson AH, Levi A (2009) Bridging classical and molecular genetics of abiotic stress resistance in cotton. Plant Genet Genomics: Crops Model 3:1–16Google Scholar
- 6.Liu F, Xu W, Wei Q, Zhang Z, Xing Z, Tan L, Di C, Yao D, Wang C, Tan Y, Yan H, Ling Y, Sun C, Xue Y, Su Z (2010) Gene expression profiles deciphering rice phenotypic variation between Nipponbare (Japonica) and 93-11 (Indica) during oxidative stress. PLoS ONE 5:e8632PubMedCentralPubMedCrossRefGoogle Scholar
- 7.Taji T, Seki M, Satou M, Sakurai T, Kobayashi M, Ishiyama K, Narusaka Y, Narusaka M, Zhu JK, Shinozaki K (2004) Comparative genomics in salt tolerance between Arabidopsis and Arabidopsis-related halophyte salt stress using Arabidopsis microarray. Plant Physiol 135:1697–1709PubMedCentralPubMedCrossRefGoogle Scholar
- 8.Fujita Y, Fujita M, Satoh R, Maruyama K, Parvez MM, Seki M, Hiratsu K, Ohme-Takagi M, Shinozaki K, Yamaguchi-Shinozaki K (2005) AREB1 is a transcription activator of novel ABRE-dependent ABA signaling that enhances drought stress tolerance in Arabidopsis. Plant Cell 17:3470–3488PubMedCentralPubMedCrossRefGoogle Scholar
- 17.Dardanelli MS, Manyani H, González-Barroso S, Rodríguez-Carvajal MA, Gil-Serrano AM, Espuny MR, López-Baena FJ, Bellogín RA, Megías M, Ollero FJ (2010) Effect of the presence of the plant growth promoting rhizobacterium (PGPR) Chryseobacterium balustinum Aur9 and salt stress in the pattern of flavonoids exuded by soybean roots. Plant Soil 328:483–493CrossRefGoogle Scholar
- 18.Radosevich SR (1987) Methods to study interactions among crops and weeds. Weed Technol 1:190Google Scholar
- 19.Evans HC (1997) Parthenium hysterophorus: a review of its weed status and the possibilities for biological control. Biocontrol News Inform 18:389–398Google Scholar
- 22.Mishra S, Mishra A, Chauhan PS, Mishra SK, Kumari M, Niranjan A, Nautiyal CS (2012) Pseudomonas putida NBRIC19 dihydrolipoamide succinyltransferase (SucB) gene controls degradation of toxic allelochemicals produced by Parthenium hysterophorus. J Appl Microbiol 112:793–808PubMedCrossRefGoogle Scholar
- 27.McCune B, Mefford MJ (2005) Multivariate analysis on the PC-ORD system. Version 5. MjM Software, Gleneden BeachGoogle Scholar
- 29.Duressa D, Soliman K, Chen D (2010) Identification of aluminum responsive genes in Al-tolerant soybean line PI 416937. Int J Plant Genomics 2010:1–13Google Scholar
- 31.Hajduch M, Rakwal R, Agrawal GK, Yonekura M, Pretova A (2001) High-resolution two-dimensional electrophoresis separation of proteins from metal stressed rice (Oryza sativa L) leaves: drastic reductions/fragmentations of ribulose-1,5-bisphosphate carboxylase/oxygenase and induction of stress-related proteins. Electrophoresis 22:2824–2831PubMedCrossRefGoogle Scholar