Individual identification of Chinese Holstein Bull by 10 STR loci
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With the premium bull plays a growing important role in cattle industry, semen detection technology based on individual identification and phylogenetic relationship is paid more and more attention. In order to lay the foundation for the establishment of the China Holstein bull identification method, this research takes 20 Chinese Holstein dairy bull’s blood and their corresponding semen, and then extracts the DNA both from the blood and semen, analysis the genetic polymorphisms of 10 microsatellite loci (TGLA227, INRA23, TGLA122, BM2113, SPS115, ETH3, ETH225, MCM158, MAF45 and UMN0108) by microsatellite marker, discuss the feasibility of this method used to individual identification. The results showed that Chinese Holstein dairy bull genetic diversity in the ten microsatellite loci were both high, and the average polymorphic information content of TGLA227, which highest, is 0.8162, ETH225 has the lowest, which is 0.6224. Use STR loci to identify the bull’s semen, the cumulative individual identification capacity is 99.99 %, which indication that 10 STR loci can be used to the frozen semen quality test and cows individual identification.
KeywordsIndividual identification STR loci Bull
This work was supported by the National Natural Science Foundation of China (No. 31000994), the Natural Science Foundation of Jiangsu Province (No. SBK201241530), the National Supporting Projects for Science and Techniques (No. 2011BAD28B02, 2012BAD12B10).
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