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Molecular Biology Reports

, Volume 41, Issue 1, pp 269–283 | Cite as

Transferability of molecular markers from major legumes to Lathyrus spp. for their application in mapping and diversity studies

  • Nuno Felipe Almeida
  • Susana Trindade Leitão
  • Constantino Caminero
  • Ana Maria Torres
  • Diego Rubiales
  • Maria Carlota Vaz Patto
Article

Abstract

Lathyrus cicera L. (chickling pea) and L. sativus L. (grass pea) have great potential among grain legumes due to their adaptability to inauspicious environments, high protein content and resistance to serious diseases. Nevertheless, due to its past underused, further activities are required to exploit this potential and to capitalise on the advances in molecular biology that enable improved Lathyrus spp. breeding programmes. In this study we evaluated the transferability of molecular markers developed for closely related legume species to Lathyrus spp. (Medicago truncatula, pea, lentil, faba bean and lupin) and tested the application of those new molecular tools on Lathyrus mapping and diversity studies. Genomic and expressed sequence tag microsatellite, intron-targeted amplified polymorphic, resistance gene analogue and defence-related gene markers were tested. In total 128 (27.7 %) and 132 (28.6 %) molecular markers were successfully cross-amplified, respectively in L. cicera and L. sativus. In total, the efficiency of transferability from genomic microsatellites was 5 %, and from gene-based markers, 55 %. For L. cicera, three cleaved amplified polymorphic sequence markers and one derived cleaved amplified polymorphic sequence marker based on the cross-amplified markers were also developed. Nine of those molecular markers were suitable for mapping in a L. cicera recombinant inbred line population. From the 17 molecular markers tested for diversity analysis, six (35 %) in L. cicera and seven (41 %) in L. sativus were polymorphic and discriminate well all the L. sativus accessions. Additionally, L. cicera accessions were clearly distinguished from L. sativus accessions. This work revealed a high number of transferable molecular markers to be used in current genomic studies in Lathyrus spp. Although their usefulness was higher on diversity studies, they represent the first steps for future comparative mapping involving these species.

Keywords

Lathyrus spp. Microsatellite Intron-targeted amplified polymorphic Resistance gene analogue Single nucleotide polymorphism Cross-genera amplification Legumes 

Notes

Acknowledgments

The authors thank the CRF-INIA, Madrid, Spain, for supplying the accessions. Mara Lisa Alves for helping in the diversity study analysis. Alberto Martín Sanz for the technical support on the primer pairs supply. This work was supported by Fundação para a Ciência e a Tecnologia through Grants #PEst-OE/EQB/LA0004/2011 and #PTDC/AGR-GPL/103285/2008. NFA and MCVP were supported by Fundação para a Ciência e a Tecnologia (SFRH/BD/44357/2008 and Research Contracts by the Ciência 2008 program respectively).

Supplementary material

11033_2013_2860_MOESM1_ESM.xlsx (52 kb)
ESM 1 Characterization of the different molecular markers tested on Lathyrus cicera and Lathyrus sativus. (XLSX 51 kb)
11033_2013_2860_MOESM2_ESM.xls (41 kb)
ESM 2 Characteristics of sequenced cross-amplified ITAPs, DRs and RGAs in Lathyrus spp. and top BLASTn scores. (XLS 41 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Nuno Felipe Almeida
    • 1
  • Susana Trindade Leitão
    • 1
  • Constantino Caminero
    • 2
  • Ana Maria Torres
    • 3
  • Diego Rubiales
    • 4
  • Maria Carlota Vaz Patto
    • 1
  1. 1.Instituto de Tecnologia Química e BiológicaUniversidade Nova de LisboaOeirasPortugal
  2. 2.Instituto Tecnológico Agrario, Consejería de Agricultura y GanaderíaJunta de Castilla y LeónValladolidSpain
  3. 3.IFAPA, Centro Alameda del ObispoCórdobaSpain
  4. 4.Institute for Sustainable AgricultureCSICCórdobaSpain

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