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Protective effects of the nuclear factor kappa B inhibitor pyrrolidine dithiocarbamate in bladder ischemia–reperfusion injury in rats

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Abstract

The aim of the present study was to evaluate the protective effects of the NF-кB inhibition with pyrrolidine-dithiocarbamate (PDTC) in ischemia–reperfusion (I/R) injury in the rat bladder. Twenty-four Sprague-Dawley male rats were divided into three groups. Group I; (n = 8) control, group II; (n = 8) I/R group; group III (n = 8) I/R and PDTC treatment. Superoxide dismutase (SOD), catalase (CAT), and gluatathione-S-transferase (GST) enzymes was studied in bladder tissue. Lipid peroxidation (as TBARS) levels in tissue homogenate were measured with thiobarbituric acid reaction. All the slides were stained with NF-кB, p53 and HSP60 immunohistochemistry for detection genome destruction and tissue stress, respectively. Our results show that the mean TBARS levels were significantly higher in group II (p < 0.05). The TBARS levels were significantly decreased in group III compared with the group II (p < 0.05). CAT, SOD and GST activities were decreased in group II, but these enzymes levels were significantly increased in group III according to the group II (p < 0.05). Under microscopic evaluation NF-кB expression increased significantly in group II compared to the group I (p < 0.05) and then decreased in group III (p < 0.05). HSP60 and p53 expression in group II was increased significantly compared with group I. Under microscopic evaluation we detected that HSP60 and p53 expression was increased significantly in group II compared with group I. In group III PDTC administration was decreased the HSP60 and p53 expression, this difference was statistically significant (p < 0.05). The results of the present study have demonstrated that NF-кB inhibition with PDTC protects and provides beneficial effects on ischemia/reperfusion stress related bladder tissue destruction.

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Abbreviations

I/R:

Ischemia/reperfusion

PDTC:

Pyrrolidine dithiocarbamate

NF-кB:

Nuclear factor-кB

HO-1:

Heme oxygenase-1

GST:

Glutathione-S-transferase

SOD:

Superoxide dismutase

CAT:

Catalase

TBARS:

Thiobarbituric acid reactive substance

H&E:

Hematoxylin eosin

HSP:

Heat shock protein

ROS:

Reactive oxygen species

MDA:

Malondialdehyde

LPO:

Lipid peroxidation

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Authors and Affiliations

  1. Department of Urology, Faculty of Medicine, Dumlupinar University, 43270, Kutahya, Turkey

    Mehmet Yucel

  2. Department of Physiology, Faculty of Medicine, Dumlupinar University, Kutahya, Turkey

    Aysegul Kucuk

  3. S.B. Etimesgut Public Health Laboratory, Ankara, Turkey

    Aslihan Cavunt Bayraktar

  4. Department of Histology and Embryology, Faculty of Medicine, Afyon Kocatepe University, Afyon, Turkey

    Murat Tosun

  5. Department of Urology, S.B. Antalya Educational and Research Hospital, Antalya, Turkey

    Soner Yalcinkaya

  6. Department of Urology, Faculty of Medicine, Dicle University, Diyarbakir, Turkey

    Namik Kemal Hatipoglu

  7. Department of Physiology, Faculty of Medicine, Osmangazi University, Eskisehir, Turkey

    Nilufer Erkasap

  8. Department of Biochemistry, Faculty of Medicine, Gazi University, Ankara, Turkey

    Mustafa Kavutcu

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  1. Mehmet Yucel
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  2. Aysegul Kucuk
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Correspondence to Mehmet Yucel.

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Yucel, M., Kucuk, A., Bayraktar, A.C. et al. Protective effects of the nuclear factor kappa B inhibitor pyrrolidine dithiocarbamate in bladder ischemia–reperfusion injury in rats. Mol Biol Rep 40, 5733–5740 (2013). https://doi.org/10.1007/s11033-013-2676-2

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  • DOI: https://doi.org/10.1007/s11033-013-2676-2

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