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Identification of human patatin-like phospholipase domain-containing protein 1 and a mutant in human cervical cancer HeLa cells

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Abstract

Recently members of mammalian patatin-like phospholipase domain containing (PNPLA) protein family have attracted attention for their critical roles in diverse aspects of lipid metabolism and signal pathway. Until now little has been known about the characteristics of PNPLA1. Here, the full length coding cDNA sequence of human PNPLA1 (hPNPLA1) was cloned for the first time, which encoded a polypeptide with 532 amino acids containing the whole patatin domain. Tissue expression profiles analysis showed that low mRNA levels of hPNPLA1 existed in various tissues, except high expression in the digestive system, bone marrow and spleen. Subcellular distribution of hPNPLA1 tagged with green fluorescence protein mainly localized to lipid droplets. Furthermore, a nonsense mutation of PNPLA1 in human cervical cancer HeLa cells was identified. The hPNPLA1 mutant encoded a protein of 412 amino acids without the C-terminal domain and did not colocalize to lipid droplets, which suggested that the C-terminal region of hPNPLA1 affected lipid droplet binding. These results identified hPNPLA1 and a mutant in HeLa cells, and provided insights into the structure and function of PNPLA1.

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Acknowledgments

This work was supported in part by Grants from the National Natural Science Foundation of China (No. 31271523) and the Natural Science Foundation Project of CQ CSTC (CSTC, 2010BB5410, 2011JJA10110, 2013jcyjA10005), and by the Program for Excellent Talents in Chongqing Higher Education Institutions (to PA Chang).

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Authors and Affiliations

  1. Key Laboratory of Molecular Biology, College of Bio-information, Chongqing University of Posts and Telecommunications, Chongqing, 400065, People’s Republic of China

    Ping-An Chang, Fei-Fei Huang, Wen-Zhen Qin, Yu-Ying Chen & Xin Zeng

  2. Laboratory of Molecular Toxicology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of China

    Yi-Jun Wu

  3. Department of Veterinary Medicine and Animal Science, Beijing University of Agriculture, Beijing, 102206, People’s Republic of China

    Ying-Jian Sun

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  1. Ping-An Chang
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  2. Ying-Jian Sun
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Correspondence to Ping-An Chang or Yi-Jun Wu.

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Chang, PA., Sun, YJ., Huang, FF. et al. Identification of human patatin-like phospholipase domain-containing protein 1 and a mutant in human cervical cancer HeLa cells. Mol Biol Rep 40, 5597–5605 (2013). https://doi.org/10.1007/s11033-013-2661-9

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  • DOI: https://doi.org/10.1007/s11033-013-2661-9

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