Molecular Biology Reports

, Volume 40, Issue 7, pp 4645–4650 | Cite as

Effects of single and combined genotypes of MC4R and POU1F1 genes on two production traits in Langshan chicken

  • Lin Jun Yan
  • Xing Tang Fang
  • Yao Liu
  • Chun Lei Zhang
  • Xuan Xuan Liu
  • Jing Zhao
  • Jing Jing Li
  • Hong Chen


The objective of this study was to analyze the effects of single and combined genotypes of MC4R and POU1F1 genes in Chinese well-known indigenous chicken (Langshan chicken) population. Genetic variants within MC4R gene and POU1F1 gene were screened through PCR-SSCP and DNA sequencing methods. A C/T mutation at nt 944 in MC4R gene (NC_006089.2:g. 944C>T) and a G/A mutation at nt 3109 in POU1F1 gene (NC_006088.2:g. 3109 G>A) were identified. Associations between the mutations of the two genes with two production traits were analyzed. The results showed that, at MC4R locus, individuals with BB and AB genotypes had highly significantly higher body weight at 16 weeks (p < 0.01) than did those with the AA genotype. And, individuals within AA and AB genotypes had significantly higher egg numbers at 300 days (p < 0.05). At POU1F1 locus, individuals with CD genotype had higher body weight at 16 weeks and egg numbers at 300 days (p < 0.05). Furthermore, combined genotypes from these two loci were found to be associated with egg numbers at 300 days (p < 0.05). The individuals within combined genotype AB/CD had higher egg production. Therefore, variations identified within the MC4R and POU1F1genes are suitable for future use in identifying chickens with the genetic potential of higher body weight and reproductive traits, at least in the population of Langshan chickens.


Langshan chicken MC4R gene POU1F1 gene Combined genotypes PCR-SSCP 



This study was supported by the Science and Technology Industrialization (Agriculture) Program of Nantong (No. CL2009002), Qing Lan Project of Jiangsu Province, A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Lin Jun Yan
    • 1
    • 2
  • Xing Tang Fang
    • 1
  • Yao Liu
    • 1
  • Chun Lei Zhang
    • 1
  • Xuan Xuan Liu
    • 1
  • Jing Zhao
    • 1
  • Jing Jing Li
    • 1
  • Hong Chen
    • 1
  1. 1.Institute of Cellular and Molecular Biology, College of Life ScienceXuzhou Normal UniversityXuzhouPeople’s Republic of China
  2. 2.Nantong Agricultural CollegeNantongPeople’s Republic of China

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