Molecular Biology Reports

, Volume 40, Issue 7, pp 4491–4498 | Cite as

No important role for genetic variation in the Chibby gene in monogenic and complex obesity

  • Jasmijn K. Van Camp
  • Doreen Zegers
  • Stijn L. Verhulst
  • Kim Van Hoorenbeeck
  • Guy Massa
  • An Verrijken
  • Kristine N. Desager
  • Luc F. Van Gaal
  • Wim Van Hul
  • Sigri Beckers


Chibby (CBY) has been identified as a potent proadipogenic factor required for adipocyte differentiation. It has been shown that CBY inhibits the canonical Wnt pathway, and therefore promotes the development of new fat cells. Our objective therefore is to investigate the contribution of rare and common genetic variation in CBY to the development of human obesity. A mutation analysis was performed on a total of 566 obese patients and 432 lean individuals. To investigate the involvement of CBY in complex obesity, we performed a genetic association analysis of the entire CBY gene region on 1,011 obese individuals and 523 control samples. Four rare, novel variants were identified in either obese patients or lean control subjects, among which two non-synonymous variations and one frameshift mutation. In addition, four previously reported CBY variants were found. In the association analysis, logistic and linear regression showed no association between common genetic variation in CBY and obesity parameters. Several novel variations were found, but no definite role in the pathogenesis of obesity could be confirmed. Results from the association analysis suggest that common variation in CBY is not a cause for obesity in the Belgian population.


Childhood obesity Wnt pathway Mutation analysis Association study 



This study was supported by a TOP-research grant from the University of Antwerp to WVH. The study was also funded by a PhD grant of the Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen) to DZ. SB holds a postdoctoral fellowship obtained from the Flemish Fund for Scientific Research (F.W.O. Vlaanderen).

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Jasmijn K. Van Camp
    • 1
  • Doreen Zegers
    • 1
  • Stijn L. Verhulst
    • 2
  • Kim Van Hoorenbeeck
    • 2
  • Guy Massa
    • 3
  • An Verrijken
    • 4
  • Kristine N. Desager
    • 2
  • Luc F. Van Gaal
    • 4
  • Wim Van Hul
    • 1
  • Sigri Beckers
    • 1
  1. 1.Department of Medical GeneticsUniversity of AntwerpAntwerpBelgium
  2. 2.Department of PaediatricsAntwerp University HospitalAntwerpBelgium
  3. 3.Department of PaediatricsJessa HospitalHasseltBelgium
  4. 4.Department of Endocrinology, Diabetology and Metabolic DiseasesAntwerp University HospitalAntwerpBelgium

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