Cloning and characterization of GPAT gene from Lepidium latifolium L.: a step towards translational research in agri-genomics for food and fuel
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Glycerol-3-phosphate acyltransferase (GPAT) catalyzes first and the rate limiting step in glycerolipid synthesis pathway, which in turn contribute to stabilization of plasma membrane structure and oil lipid synthesis in plant cells. Here, we report cloning and characterization of GPAT gene from Lepidium latifolium (LlaGPAT). The cDNA sequence (1,615 bp) of LlaGPAT gene consisted of 1,113 bp ORF encoding a protein of 370 aa residues, with deduced mass of 41.2 kDa and four acyltransferase (AT) motifs having role in catalysis and in glycerol-3-phosphate binding. Southern blot analysis suggested presence of a single copy of the gene in the genome. Tissue specific expression of the gene was seen more abundantly in aerial parts, compared to the roots. Quantitative real-time PCR indicated down-regulation of the gene by cold (4 °C), drought (PEG6000), salt (300 mM NaCl) and ABA (100 μM) treatments. Considering the vitality of the function of encoded enzyme, LlaGPAT can be considered a potential candidate gene for genetic engineering of oil yields and abiotic stress management in food as well as fuel crops.
KeywordsAbiotic stress GPAT Triacylglycerols Acyltransferase Lepidium latifolium
Poly ethylene glycol
Rapid amplification of cDNA ends
Defence Research and Development Organisation (DRDO), HQ, New Delhi is duly acknowledged for funding the project and providing Senior Research Fellowship (SRF) to PP and SS.
- 5.Liu JM, Chen SN, Yan B, Huang XQ, Yang MZ (1999) Cloning and sequencing of the cDNA (Accession No. AF090734) coding for glycerol-3-phosphate acyltransferase from Vicia faba. Plant Physiol 20:934Google Scholar
- 10.Akbar E, Yakoob Z, Kamarudin SK, Ismail M, Salimon J (2009) Characteristic and composition of Jatropha curcas oil seed from Malaysia and its potential as biodiesel feedstock. Eur J Sci Res 91:162–163Google Scholar
- 22.Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbour, New YorkGoogle Scholar
- 28.Chen N, Guo SJ, Yan K, Dong XC, Meng QW (2005) Cloning and expression analysis of glycerol-3-phosphate acyltransferase gene from sweet pepper. Acta Horticulturae Sinica 32:823–827 (in Chinese)Google Scholar