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Molecular Biology Reports

, Volume 40, Issue 4, pp 2829–2835 | Cite as

Identification of novel SNPs in the Sarda breed goats POU1F1 gene and their association with milk productive performance

  • Cinzia Daga
  • Marta Paludo
  • Sebastiano Luridiana
  • Maria Consuelo Mura
  • Sara Bodano
  • Michele Pazzola
  • Maria Luisa Dettori
  • Giuseppe Massimo Vacca
  • Vincenzo Carcangiu
Article

Abstract

The aim of the study was to detect polymorphism in the POU1F1 gene in Sarda breed goat, as well as to establish if SNPs could be associated with milk productive traits. The research was conducted on 129 Sarda breed goats from 4 to 5 years old, multiparous, lactating and in their third to fifth lactation. We report nine exonic and seven non-coding regions SNPs within the Sarda goat POU1F1 gene, namely, Ex 1 61 G>C; Ex 1 108 G>A; Ex 3 C>T; Ex 3 92 C>T; Ex 4 110 A>G; Ex 5 34 G>A resulting in Arg213Lys change; IVS4 641 G>A, IVS4 643 A>C, IVS4 659 G>A, IVS4 677 A>C, IVS4 G699Del, IVS4 709 C>G, Ex 6 17 G>A resulting in Arg228Ser change, Ex 6 58 G>T, Ex 6 172 T>C, 3′UTR 110 T>C. A statistically significant association was found between genotype TT, in position 17 of the exon 6 (3.1 % of frequency), and increased milk yield (P < 0.01) while genotype GT (25.6 % of frequency) was associated with a higher fat content. Genotype TT in position 58 of the exon 6 (3.9 % of frequency) was found to be associated with a higher fat (P < 0.01) and protein content (P < 0.05). Twenty-eight haplotypes were detected, but no significant association between the haplotypes and the milk production traits have been found. Our data, as well as providing new SNPs extending the POU1F1 gene characterization, evidence a relationship between polymorphism and milk production traits in Sarda goat breed.

Keywords

POU1F1 gene SNPs Sarda goat breed Milk productive trait 

Notes

Acknowledgments

This study is supported by the Foundation of Banco di Sardegna.

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Cinzia Daga
    • 1
  • Marta Paludo
    • 1
  • Sebastiano Luridiana
    • 1
  • Maria Consuelo Mura
    • 1
  • Sara Bodano
    • 1
  • Michele Pazzola
    • 1
  • Maria Luisa Dettori
    • 1
  • Giuseppe Massimo Vacca
    • 1
  • Vincenzo Carcangiu
    • 1
  1. 1.Dipartimento di Medicina VeterinariaUniversità di SassariSassariItaly

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