Association of CAST gene polymorphisms with carcass and meat quality traits in Yanbian cattle of China
Bovine calpastatin (CAST) gene polymorphisms have been associated with meat tenderness traits; however, little is known about how the CAST gene affects beef quality traits. In this study, 25 single nucleotide polymorphisms were detected in the CAST gene using polymerase chain reaction with single-strand conformation polymorphism and gene sequencing. Different preponderant genotypes were found at the corresponding gene locus. The E1-1, E1-2, and C3-1 loci were correlated with meat tenderness height and highly correlated with the fatty acid content and the amino acid content. The E4-2 locus was not correlated with meat tenderness, but it was correlated with cooking loss, brightness, and yellowness, among others. The CAST gene is a potential marker for these meat quality traits, but further research is required.
KeywordsCattle Calpastatin Polymorphisms SNP Meat quality
This work was supported by the National High Technology Research and Development Project of China (No.2008AA101010) and National Key Technology R&D Program in the 11th Five year Plan of china (2008BADB2B01). The foundation (National High Technology Research and Development Project of China (No.2008AA101010) and National Key Technology R&D Program in the 11th Five year Plan of china (2008BADB2B01) will be replaced by National Natural Science Foundation of China (Analysis of the genes function with meat quality in Yanbian cattle, Grant No:31160442).
- 7.White S, Casas E, Wheeler T, Shackelford S, Koohmaraie M, Riley D, Chase C Jr, Johnson D, Keele J, Smith T (2005) A new single nucleotide polymorphism in CAPN1 extends the current tenderness marker test to include cattle of Bos indicus, Bos taurus, and crossbred descent. J Anim Sci 83(9):2001–2008PubMedGoogle Scholar
- 8.Chung E, Kim W, Kim Y, Han S (2000) DNA fingerprinting of Korean cattle using AFLP markers. Korean J Anim Sci 42(4):391–406Google Scholar
- 11.Choi B, Ahn B, Kook K, Sun S, Myung K, Moon S, Kim J (2002) Effects of feeding patterns and sexes on growth rate, carcass trait and grade in Korean native cattle. Asian Australas J Anim Sci 15(6):838–843Google Scholar
- 16.Casas E, White S, Riley D, Smith T, Brenneman R, Olson T, Johnson D, Coleman S, Bennett G, Chase C Jr (2005) Assessment of single nucleotide polymorphisms in genes residing on chromosomes 14 and 29 for association with carcass composition traits in Bos indicus cattle. J Anim Sci 83(1):13–19PubMedGoogle Scholar
- 27.Morris C, Cullen N, Hickey S, Dobbie P, Veenvliet B, Manley T, Pitchford WS, Kruk ZA, Bottema CDK, Wilson T (2006) Genotypic effects of calpain 1 and calpastatin on the tenderness of cooked M. longissimus dorsi steaks from Jersey × Limousin, Angus and Hereford-cross cattle. Anim Genet 37(4):411–414PubMedCrossRefGoogle Scholar