Molecular Biology Reports

, Volume 40, Issue 2, pp 787–796 | Cite as

Identification and characterization of promoter and regulatory regions for mouse Adam2 gene expression

  • Heejin Choi
  • Boyeon Lee
  • Sora Jin
  • Jun Tae Kwon
  • Jihye Kim
  • Juri Jeong
  • Seungmin Oh
  • Byung-Nam Cho
  • Zee Yong Park
  • Chunghee Cho


ADAM2, a member of the ‘a disintegrin and metalloprotease’ (ADAM) family, is a key protein in mammalian fertilization that is specifically expressed in testicular germ cells. Here, we investigated the transcriptional regulation of the mouse Adam2 gene. An in silico analysis identified two conserved non-coding sequences located upstream of the mouse and human ADAM2 genes. The upstream region of the mouse Adam2 gene was found to lack typical TATA and CAAT boxes, and to have a high GC content. Our in vitro transient transfection-reporter analysis identified a promoter in this region of the mouse Adam2 gene, along with regulatory regions that inhibit the activity of this promoter in somatic cells. Site-directed mutagenesis revealed that the caudal-type homeobox 1 and CCTC-binding factor motifs are responsible for the inhibitory activities of the repressor regions. Finally, electrophoretic mobility shift assays showed putative transcription factor–promoter DNA complexes, and DNA-affinity chromatography and proteomic analyses identified myelin gene regulatory factor as a binding partner of the Adam2 promoter. This provides the first identification and characterization of promoter and repressor regions that regulate the transcription of the mouse Adam2 gene, and offers insights into the regulation of this germ-cell-specific gene.


ADAM2 Germ cell Testis Transcription factor Transcriptional repressor 



This work was supported by the Korea Science and Engineering Foundation (Grant 2010-0028776), the Korea Research Foundation (Grant KRF-2008-313-C00736), and a Gwangju Institute of Science and Technology Systems Biology Infrastructure Establishment Grant.


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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Heejin Choi
    • 1
  • Boyeon Lee
    • 1
  • Sora Jin
    • 1
  • Jun Tae Kwon
    • 1
  • Jihye Kim
    • 1
  • Juri Jeong
    • 1
  • Seungmin Oh
    • 1
  • Byung-Nam Cho
    • 2
  • Zee Yong Park
    • 1
  • Chunghee Cho
    • 1
  1. 1.School of Life SciencesGwangju Institute of Science and TechnologyGwangjuKorea
  2. 2.Department of Life ScienceThe Catholic University of KoreaBucheonKorea

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