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Molecular Biology Reports

, Volume 40, Issue 1, pp 697–705 | Cite as

Molecular cloning and characterization of a cDNA encoding kiwifruit l-myo-inositol-1-phosphate synthase, a key gene of inositol formation

  • Meng Cui
  • Dong Liang
  • Fengwang Ma
Article

Abstract

l-myo-inositol-1-phosphate synthase (MIPS; EC 5.5.1.4) is the key enzyme involved in de novo synthesis of myo-inositol, leading to numerous cellular functions. We isolated an open reading frame of Actinidia deliciosa MIPS (AdMIPS), which is 1,533 bp long and codes for 510 amino acids, with a predicted molecular weight of 56.3 kDa. Sequence analysis revealed its high similarity with MIPS proteins from other organisms. Gene expression and enzyme activity were highest in flower and young fruit. Transcription of AdMIPS was also detected in other tissues. Moderate drought drastically induced expression in the leaves whereas salinity stress induced transcription and enzyme activity in the leaves, phloem, and roots with different degrees. However, a longer period of saline exposure suppressed both expression and enzyme activity in all sampled tissues, indicating that AdMIPS is salt-sensitive.

Keywords

Actinidia deliciosa Developmental stages Drought Enzyme activity MIPS Salinity 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (30871700). The authors are grateful to Priscilla Licht for help in revising our English composition.

References

  1. 1.
    Majumder AL, Johnson MD, Henry SA (1997) l-myo-Inositol-1-phosphate synthase. Biochim Biophys Acta (BBA)-Lipids and Lipid. Metabolism 1348(1–2):245–256Google Scholar
  2. 2.
    Loewus FA, Loewus MW (1983) Myo-inositol: its biosynthesis and metabolism. Annu Rev Plant Physiol 34(1):137–161CrossRefGoogle Scholar
  3. 3.
    Loewus FA, Murthy PPN (2000) Myo-Inositol metabolism in plants. Plant Sci 150(1):1–19CrossRefGoogle Scholar
  4. 4.
    Lorence A, Chevone BI, Mendes P, Nessler CL (2004) Myo-Inositol oxygenase offers a possible entry point into plant ascorbate biosynthesis. Plant Physiol 134(3):1200–1205PubMedCrossRefGoogle Scholar
  5. 5.
    Donahue JL, Alford SR, Torabinejad J, Kerwin RE, Nourbakhsh A, Ray WK, Hernick M, Huang X, Lyons BM, Hein PP (2010) The Arabidopsis thaliana myo-inositol 1-phosphate synthase1 gene is required for myo-inositol synthesis and suppression of cell death. Plant Cell 22(3):888–903PubMedCrossRefGoogle Scholar
  6. 6.
    Kanter U, Usadel B, Guerineau F, Li Y, Pauly M, Tenhaken R (2005) The inositol oxygenase gene family of Arabidopsis is involved in the biosynthesis of nucleotide sugar precursors for cell-wall matrix polysaccharides. Planta 221(2):243–254PubMedCrossRefGoogle Scholar
  7. 7.
    Raboy V (2003) Myo-Inositol-1,2,3,4,5,6-hexakisphosphate. Phytochemistry 64(6):1033–1043PubMedCrossRefGoogle Scholar
  8. 8.
    Abreu EFM, Aragão FJL (2007) Isolation and characterization of a myo-inositol-1-phosphate synthase gene from yellow passion fruit (Passiflora edulis f. flavicarpa) expressed during seed development and environmental stress. Ann Bot 99(2):285–292PubMedCrossRefGoogle Scholar
  9. 9.
    RayChaudhuri A, Hait NC, DasGupta S, Bhaduri TJ, Deb R, Majumder AL (1997) l-myo-lnositol 1-phosphate synthase from plant sources (characteristics of the chloroplastic and cytosolic enzymes). Plant Physiol 115(2):727–736PubMedGoogle Scholar
  10. 10.
    Kaur H, Shukla RK, Yadav G, Chattopadhyay D, Majee M (2008) Two divergent genes encoding l-myo-inositol 1-phosphate synthase1 (CaMIPS1) and 2 (CaMIPS2) are differentially expressed in chickpea. Plant Cell Environ 31(11):1701–1716PubMedCrossRefGoogle Scholar
  11. 11.
    Smart CC, Fleming AJ (1993) A plant gene with homology to d-myo-inositol-3-phosphate synthase is rapidly and spatially up-regulated during an abscisic acid-induced morphogenic response in Spirodela polyrrhiza. Plant J 4(2):279–293PubMedCrossRefGoogle Scholar
  12. 12.
    Chun JA, Jin UH, Lee JW, Yi YB, Hyung NI, Kang MH, Pyee JH, Suh M, Kang CW, Seo HY (2003) Isolation and characterization of a myo-inositol 1-phosphate synthase cDNA from developing sesame (Sesamum indicum L.) seeds: functional and differential expression, and salt-induced transcription during germination. Planta 216(5):874–880PubMedGoogle Scholar
  13. 13.
    Wang Y, Huang J, Gou CB, Dai X, Chen F, Wei W (2011) Cloning and characterization of a differentially expressed cDNA encoding myo-inositol-1-phosphate synthase involved in response to abiotic stress in Jatropha curcas. Plant Cell Tissue Organ 106(2):269–277CrossRefGoogle Scholar
  14. 14.
    Das-Chatterjee A, Goswami L, Maitra S, Dastidar K, Ray S, Majumder A (2006) Introgression of a novel salt-tolerant l-myo-inositol 1-phosphate synthase from Porteresia coarctata (Roxb.) Tateoka (PcINO1) confers salt tolerance to evolutionary diverse organisms. FEBS Lett 580(16):3980PubMedCrossRefGoogle Scholar
  15. 15.
    Nelson DE, Rammesmayer G, Bohnert HJ (1998) Regulation of cell-specific inositol metabolism and transport in plant salinity tolerance. Plant Cell 10(5):753–764PubMedGoogle Scholar
  16. 16.
    Patra B, Ray S, Richter A, Majumder AL (2010) Enhanced salt tolerance of transgenic tobacco plants by co-expression of PcINO1 and McIMT1 is accompanied by increased level of myo-inositol and methylated inositol. Protoplasma 245(1):143–152PubMedCrossRefGoogle Scholar
  17. 17.
    Bieleski RL, Clark CJ, Klages KU (1997) Identification of myo-inositol as a major carbohydrate in kiwifruit, Actinidia deliciosa. Phytochemistry 46(1):51–55CrossRefGoogle Scholar
  18. 18.
    Pardo JM (2010) Biotechnology of water and salinity stress tolerance. Curr Opin Biotechnol 21(2):185–196PubMedCrossRefGoogle Scholar
  19. 19.
    Judd M, McAneney K, Wilson K (1989) Influence of water stress on kiwifruit growth. Irrigation Sci 10(4):303–311CrossRefGoogle Scholar
  20. 20.
    Klages K, Boldingh H, Smith G (1999) Accumulation of myo-inositol in Actinidia seedlings subjected to salt stress. Ann Bot 84(4):521–527CrossRefGoogle Scholar
  21. 21.
    Wang Y, Ma F, Li M, Liang D, Zou J (2011) Physiological responses of kiwifruit plants to exogenous ABA under drought conditions. Plant Growth Regul 64(1):63–74CrossRefGoogle Scholar
  22. 22.
    Chang S, Puryear J, Cairney J (1993) A simple and efficient method for isolating RNA from pine trees. Plant Mol Biol Rep 11(2):113–116CrossRefGoogle Scholar
  23. 23.
    Crowhurst RN, Gleave AP, MacRae EA, Ampomah-Dwamena C, Atkinson RG, Beuning LL, Bulley SM, Chagne D, Marsh KB, Matich AJ (2008) Analysis of expressed sequence tags from Actinidia: applications of a cross species EST database for gene discovery in the areas of flavor, health, color and ripening. BMC genomics 9(1):351PubMedCrossRefGoogle Scholar
  24. 24.
    Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22(22):4673–4680PubMedCrossRefGoogle Scholar
  25. 25.
    Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28(10):2731–2739PubMedCrossRefGoogle Scholar
  26. 26.
    Barnett J, Brice R, Corina D (1970) A colorimetric determination of inositol monophosphates as an assay for d-glucose 6-phosphate-1l-myoinositol 1-phosphate cyclase. Biochem J 119(2):183PubMedGoogle Scholar
  27. 27.
    Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72(1–2):248–254PubMedCrossRefGoogle Scholar
  28. 28.
    Majumder AL, Chatterjee A, Ghosh Dastidar K, Majee M (2003) Diversification and evolution of l-myo-inositol 1-phosphate synthase. FEBS Lett 553(1–2):3–10Google Scholar
  29. 29.
    Chen L, Zhou C, Yang H, Roberts MF (2000) Inositol-1-phosphate synthase from Archaeoglobus fulgidus is a class II aldolase. Biochemistry 39(40):12415–12423PubMedCrossRefGoogle Scholar
  30. 30.
    Raboy V (2009) Approaches and challenges to engineering seed phytate and total phosphorus. Plant Sci 177(4):281–296CrossRefGoogle Scholar
  31. 31.
    Boldingh H, Smith G, Klages K (2000) Seasonal concentrations of non-structural carbohydrates of five Actinidia species in fruit, leaf and fine root tissue. Ann Bot 85(4):469–476CrossRefGoogle Scholar
  32. 32.
    Klages K, Donnison H, Boldingh H, MacRae E (1998) Myo-Inositol is the major sugar in Actinidia arguta during early fruit development. Funct Plant Biol 25(1):61–68Google Scholar
  33. 33.
    Ishitani M, Majumder AL, Bornhouser A, Michalowski CB, Jensen RG, Bohnert HJ (1996) Coordinate transcriptional induction of myo-inositol metabolism during environmental stress. Plant J 9(4):537–548PubMedCrossRefGoogle Scholar
  34. 34.
    Larson S, Raboy V (1999) Linkage mapping of maize and barley myo-inositol 1-phosphate synthase DNA sequences: correspondence with a low phytic acid mutation. Theor Appl Genet 99(1):27–36CrossRefGoogle Scholar
  35. 35.
    Hegeman CE, Good LL, Grabau EA (2001) Expression of d-myo-inositol-3-phosphate synthase in soybean. Implications for phytic acid biosynthesis. Plant Physiol 125(4):1941–1948PubMedCrossRefGoogle Scholar
  36. 36.
    Johnson MD, Sussex IM (1995) 1l-myo-Inositol 1-Phosphate Synthase from Arabidopsis thaliana. Plant Physiol 107(2):613–619PubMedGoogle Scholar
  37. 37.
    Keller R, Brearley CA, Trethewey RN, Müller-Röber B (1998) Reduced inositol content and altered morphology in transgenic potato plants inhibited for 1d myo-inositol 3-phosphate synthase. Plant J 16(4):403–410CrossRefGoogle Scholar
  38. 38.
    Majee M, Maitra S, Dastidar KG, Pattnaik S, Chatterjee A, Hait NC, Das KP, Majumder AL (2004) A novel salt-tolerant l-myo-inositol-1-phosphate synthase from Porteresia coarctata (Roxb.) Tateoka, a halophytic wild rice. J Biol Chem 279(27):28539PubMedCrossRefGoogle Scholar
  39. 39.
    Vinocur B, Altman A (2005) Recent advances in engineering plant tolerance to abiotic stress: achievements and limitations. Curr Opin Biotech 16(2):123–132PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  1. 1.State Key Laboratory of Crop Stress Biology in Arid AreasCollege of Horticulture, Northwest A&F UniversityYanglingChina

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