Genotype and haplotype analysis of the AZGP1 gene in cattle
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Zinc-a2-glycoprotien (AZGP1) involved in lipid metabolism and associated with adipose tissue atrophy in cachexia. And it also related to sperm motility and in turn fertilization. To ascertain whether there were mutations in the bovine AZGP1 gene, this study investigated variation of the AZGP1 gene through PCR-SSCP and sequencing. Four missense mutations were identified in 649 cattle from six independent populations. Haplotype frequencies and linkage disequilibrium (LD) coefficients of these SNPs in three Chinese indigenous cattle breeds were analyzed. One LD block was found in three cattle breeds. The statistical analyses indicated that AC genotype of Z4 locus was associated with the high body weight, body length and chest girth in Jiaxian cattle breed (P < 0.05). Our results provided evidence that polymorphisms in the AZGP1 gene were associated with growth traits, and may be used for marker-assisted selection and management in cattle breeding program.
KeywordsCattle AZGP1 gene Mutation Genotype Association
This study was supported by the National Natural Science Foundation of China (No. 30972080), Program of National Beef Cattle Industrial Technology System (No. CARS-38), Agricultural Science and Technology Innovation Projects of Shaanxi Province (No. 2012NKC01-13).
- 2.Bing C, Bao Y, Jenkins J, Sanders P, Manieri M, Cinti S, Tisdale MJ, Trayhurn P (2004) Zinc-alpha 2-glycoprotein, a lipid mobilizing factor, is expressed in adipocytes and is up-regulated in mice with cancer cachexia. Proc Natl Acad Sci USA 101(8):2500–2505. doi: 10.1073/pnas.0308647100 PubMedCrossRefGoogle Scholar
- 4.Gohda T, Makita Y, Shike T, Tanimoto M, Funabiki K, Horikoshi S, Tomino Y (2003) Identification of epistatic interaction involved in obesity using the KK/Ta mouse as a type 2 diabetes model–Is Zn-alpha(2) glycoprotein-1 a candidate gene for obesity? Diabetes 52(8):2175–2181PubMedCrossRefGoogle Scholar
- 15.Stephens JC (2001) Haplotype variation and linkage disequilibrium in 313 human genes (vol 293, pg 489, 2001). Science 293(5532):1048Google Scholar
- 17.Yamasaki M, Tenaillon MI, Bi IV, Schroeder SG, Sanchez-Villeda H, Doebley JF, Gaut BS, McMullen MD (2005) A large-scale screen for artificial selection in maize identifies candidate agronomic loci for domestication and crop improvement. Plant Cell 17(11):2859–2872. doi: 10.1105/tpc.105.037242 PubMedCrossRefGoogle Scholar