Molecular Biology Reports

, Volume 39, Issue 12, pp 10475–10479 | Cite as

Genotype and haplotype analysis of the AZGP1 gene in cattle

  • Bao Zhang
  • Yi Kun Guo
  • Shengbin Li
  • Liang Zhi Zhang
  • Xian Yong Lan
  • Shen Rong Hu
  • Hong Chen


Zinc-a2-glycoprotien (AZGP1) involved in lipid metabolism and associated with adipose tissue atrophy in cachexia. And it also related to sperm motility and in turn fertilization. To ascertain whether there were mutations in the bovine AZGP1 gene, this study investigated variation of the AZGP1 gene through PCR-SSCP and sequencing. Four missense mutations were identified in 649 cattle from six independent populations. Haplotype frequencies and linkage disequilibrium (LD) coefficients of these SNPs in three Chinese indigenous cattle breeds were analyzed. One LD block was found in three cattle breeds. The statistical analyses indicated that AC genotype of Z4 locus was associated with the high body weight, body length and chest girth in Jiaxian cattle breed (P < 0.05). Our results provided evidence that polymorphisms in the AZGP1 gene were associated with growth traits, and may be used for marker-assisted selection and management in cattle breeding program.


Cattle AZGP1 gene Mutation Genotype Association 



This study was supported by the National Natural Science Foundation of China (No. 30972080), Program of National Beef Cattle Industrial Technology System (No. CARS-38), Agricultural Science and Technology Innovation Projects of Shaanxi Province (No. 2012NKC01-13).


  1. 1.
    Bao Y, Bing C, Hunter L, Jenkins JR, Wabitsch M, Trayhurn P (2005) Zinc-[alpha]2-glycoprotein, a lipid mobilizing factor, is expressed and secreted by human (SGBS) adipocytes. FEBS Lett 579(1):41–47PubMedCrossRefGoogle Scholar
  2. 2.
    Bing C, Bao Y, Jenkins J, Sanders P, Manieri M, Cinti S, Tisdale MJ, Trayhurn P (2004) Zinc-alpha 2-glycoprotein, a lipid mobilizing factor, is expressed in adipocytes and is up-regulated in mice with cancer cachexia. Proc Natl Acad Sci USA 101(8):2500–2505. doi: 10.1073/pnas.0308647100 PubMedCrossRefGoogle Scholar
  3. 3.
    Bing C, Trayhurn P (2009) New insights into adipose tissue atrophy in cancer cachexia. Proc Nutr Soc 68(4):385–392. doi: 10.1017/s0029665109990267 PubMedCrossRefGoogle Scholar
  4. 4.
    Gohda T, Makita Y, Shike T, Tanimoto M, Funabiki K, Horikoshi S, Tomino Y (2003) Identification of epistatic interaction involved in obesity using the KK/Ta mouse as a type 2 diabetes model–Is Zn-alpha(2) glycoprotein-1 a candidate gene for obesity? Diabetes 52(8):2175–2181PubMedCrossRefGoogle Scholar
  5. 5.
    Russell ST, Hirai K, Tisdale MJ (2002) Role of beta 3-adrenergic receptors in the action of a tumour lipid mobilizing factor. Br J Cancer 86(3):424–428. doi: 10.1038/sj/bjc/6600086 PubMedCrossRefGoogle Scholar
  6. 6.
    Bing C, Mracek T, Gao D, Trayhurn P (2010) Zinc-alpha 2-glycoprotein: an adipokine modulator of body fat mass? Int J Obes 34(11):1559–1565. doi: 10.1038/ijo.2010.105 CrossRefGoogle Scholar
  7. 7.
    Hassan MI, Waheed A, Yadav S, Singh TP, Ahmad F (2008) Zinc alpha 2-glycoprotein: a multidisciplinary protein. Mol Cancer Res 6(6):892–906. doi: 10.1158/1541-7786.mcr-07-2195 PubMedCrossRefGoogle Scholar
  8. 8.
    Frenette G, Thabet M, Sullivan R (2006) Polyol pathway in human epididymis and semen. J Androl 27(2):233–239. doi: 10.2164/jandrol.05108 PubMedCrossRefGoogle Scholar
  9. 9.
    Ding ZD, Qu F, Guo W, Ying XQ, Wu MZ, Zhang YL (2007) Identification of sperm forward motility-related proteins in human seminal plasma. Mol Reprod Dev 74:1124–1131. doi: 10.1002/mrd.20624 PubMedCrossRefGoogle Scholar
  10. 10.
    Mullenbach R, Lagoda PJL, Welter C (1989) An efficient salt-chloroform extraction of DNA from blood and tissues. Trends Genet 5(12):391PubMedGoogle Scholar
  11. 11.
    Nei M, Roychoud Ak (1974) Sampling variances of heterozygosity and genetic distance. Genetics 76(2):379–390PubMedGoogle Scholar
  12. 12.
    Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic-linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32(3):314–331PubMedGoogle Scholar
  13. 13.
    Barrett JC, Fry B, Maller J, Daly MJ (2005) Haploview: analysis and visualization of LD and haplotype maps. Bioinformatics 21(2):263–265. doi: 10.1093/bioinformatics/bth457 PubMedCrossRefGoogle Scholar
  14. 14.
    Conrad DF, Jakobsson M, Coop G, Wen X, Wall JD, Rosenberg NA, Pritchard JK (2006) A worldwide survey of haplotype variation and linkage disequilibrium in the human genome. Nat Genet 38(11):1251–1260PubMedCrossRefGoogle Scholar
  15. 15.
    Stephens JC (2001) Haplotype variation and linkage disequilibrium in 313 human genes (vol 293, pg 489, 2001). Science 293(5532):1048Google Scholar
  16. 16.
    Zhou M, Lei M, Rao Y, Nie Q, Zeng H, Xia M, Liang F, Zhang D, Zhang X (2008) Polymorphisms of vasoactive intestinal peptide receptor-1 gene and their genetic effects on broodiness in chickens. Poult Sci 87(5):893–903. doi: 10.3382/ps.2007-00495 PubMedCrossRefGoogle Scholar
  17. 17.
    Yamasaki M, Tenaillon MI, Bi IV, Schroeder SG, Sanchez-Villeda H, Doebley JF, Gaut BS, McMullen MD (2005) A large-scale screen for artificial selection in maize identifies candidate agronomic loci for domestication and crop improvement. Plant Cell 17(11):2859–2872. doi: 10.1105/tpc.105.037242 PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Bao Zhang
    • 1
    • 2
    • 3
  • Yi Kun Guo
    • 1
  • Shengbin Li
    • 2
  • Liang Zhi Zhang
    • 1
  • Xian Yong Lan
    • 1
  • Shen Rong Hu
    • 1
  • Hong Chen
    • 1
  1. 1.College of Animal Science and TechnologyNorthwest A&F University, Shaanxi Key Laboratory of Molecular Biology for AgricultureYanglingPeople’s Republic of China
  2. 2.Key Laboratory of National Ministry of Health for Forensic SciencesCollege of Medicine, Xi’an Jiaotong UniversityXi’anPeople’s Republic of China
  3. 3.Key Laboratory of Environment and Genes Related to DiseasesXi’an Jiaotong University, Ministry of EducationXi’anPeople’s Republic of China

Personalised recommendations