Molecular Biology Reports

, Volume 39, Issue 12, pp 10331–10338 | Cite as

Genetic polymorphisms of lipoprotein lipase gene and their associations with growth traits in Xiangxi cattle

  • Xing Ping Wang
  • Zhuo Ma Luoreng
  • Feng Li
  • Jin Ren Wang
  • Na Li
  • Shu Hong Li


Lipoprotein lipase (LPL), involved in the metabolism and transport of lipids, regulate energy balance, fat deposition and growth traits. The objective of this study was to investigate the single nucleotide polymorphisms (SNPs) of LPL gene and to determine their associations between these polymorphisms and growth traits in Xiangxi cattle breed. In this study, six novel SNPs (C355157T, T355169C, T355186G, A355210G, T355348A and T355420C) and one reported SNP (A355427T, has been recorded in dbSNP, ID rs110590698) were detected using polymerase chain reaction and DNA sequencing method. Genotyping and genetic diversity analysis were performed in 240 Xiangxi cattle on the basis of sequence alignment, which indicated that five SNPs (C355157T, 355186G, T355348A, T355420C, A355427T) were in abundant genetic diversity, and the other two SNPs (T355169C and TA355210G) were in low genetic diversity. Linkage disequilibrium analysis showed that 18 different haplotypes were identified in these animals. Moreover, the results of the association between LPL gene polymorphisms and growth traits indicated that the individuals with H1H1 haplotype combination had higher BW and HG than those with other haplotype combinations (P < 0.05). The animals with CC genotype maintain higher mean values for BW than those with the CT and TT genotypes (P < 0.05) at T355420C locus. The animals with the AA genotype have lower mean values for WH, BL, HG and BW than those with the AT and TT genotypes at A355427T locus (P < 0.05). The results suggested that the SNPs of the LPL gene might be useful genetic markers for growth traits in the bovine reproduction and breeding.


Cattle LPL gene Polymorphism Growth traits Association analysis 



We also thank the reviewers and editor for helpful comments. This work was supported by the Science–Technology Program Research Foundation of Hunan Province, China (Grant No. 2010NK3043), the Natural Science Foundation of Hunan Province, China (Grant No. 11JJ4020), the Open Fund Project Laboratory in Hunan Universities, China (Grant No. 11K045), the Ph D Introduction Program of Hunan University of Arts and Science (Grant No. BSQD1002).


  1. 1.
    Takahashi S, Suzuki J, Kohno M, Oida K, Tamai T, Miyabo S, Yamamoto T, Nakai T (1995) Enhancement of the binding of triglyceride-rich lipoproteins to the very low density lipoprotein receptor by apolipoprotein E and lipoprotein lipase. J Biol Chem 270(2):15747–15754PubMedCrossRefGoogle Scholar
  2. 2.
    Goldberg IJ, Merkel M (2001) Lipoprotein lipase: physiology, biochemistry, and molecular biology. Front Biosci 6:D388–D405PubMedCrossRefGoogle Scholar
  3. 3.
    Auwerx J, Leroy P, Schoonjans K (1992) Lipoprotein lipase: recent contributions from molecular biology. Crit Rev Clin Lab Sci 29(3–4):243–268PubMedCrossRefGoogle Scholar
  4. 4.
    Wang H, Knaub LA, Jensen DR, Young JD, Hong EG, Ko HJ, Coates AM, Goldberg IJ, de la Houssaye BA, Janssen RC, McCurdy CE, Rahman SM, Soo CC, Shulman GI, Kim JK, Friedman JE, Eckel RH (2009) Skeletal muscle-specific deletion of lipoprotein lipase enhances insulin signaling in skeletal muscle but causes insulin resistance in liver and other tissues. Diabetes 58(1):116–124PubMedCrossRefGoogle Scholar
  5. 5.
    Wang H, Eckel RH (2009) Lipoprotein lipase: from gene to obesity. Am J Physiol Endocrinol Metab 297:E271–E288PubMedCrossRefGoogle Scholar
  6. 6.
    Lu JZ, Li JX, Ji CN, Yu WY, Xu ZY, Huang SD (2008) Expression of lipoprotein lipase associated with lung adenocarcinoma tissues. Mol Biol Rep 35(1):59–63PubMedCrossRefGoogle Scholar
  7. 7.
    Ferland A, Château-Degat ML, Hernandez TL, Eckel RH (2012) Tissue-specific responses of lipoprotein lipase to dietary macronutrient composition as a predictor of weight gain over 4 years. Obesity (Silver Spring) 20(5):1006–1011CrossRefGoogle Scholar
  8. 8.
    Kim KS, Thomsen H, Bastiaansen J, Nguyen NT, Dekkers JC, Plastow GS, Rothschild MF (2004) Investigation of obesity candidate genes on porcine fat deposition quantitative trait loci regions. Obes Res 12(12):1981–1994PubMedCrossRefGoogle Scholar
  9. 9.
    Badaoui B, Serradilla JM, Tomas A, Urrutia J, Ares BL, Carrizosa J, Sanche A, Jordana J, Amills M (2010) Short communication: identification of two polymorphisms in the goat lipoprotein lipase gene and their association with milk production traits. J Dairy Sci 90(6):3012–3017CrossRefGoogle Scholar
  10. 10.
    Radha V, Mohan V, Vidya R, Ashok AK, Deepa R, Mathias RA (2006) Association of lipoprotein lipase Hind III and Ser 447 Ter polymorphisms with dyslipidemia in Asian Indians. Am J Cardiol 97(9):1337–1342PubMedCrossRefGoogle Scholar
  11. 11.
    Jemaa R, Tuzet S, Portos C, Betoulle D, Apfelbaum M, Fumeron F (1995) Lipoprotein lipase gene polymorphisms: associations with hypertriglyceridemia and body mass index in obese people. Int J Obes Relat Metab Disord 19(4):270–274PubMedGoogle Scholar
  12. 12.
    Ding XZ, Liang CN, Guo X, Xing CF, Bao PJ, Chu M, Pei J, Zhu XS, Yan P (2012) A novel single nucleotide polymorphism in exon 7 of LPL gene and its association with carcass traits and visceral fat deposition in yak (Bos grunniens) steers. Mol Biol Rep 39(1):669–673PubMedCrossRefGoogle Scholar
  13. 13.
    Zhang RF, Li XF (2011) Association between IGF-IR, m-calpain and UCP-3 gene polymorphisms and growth traits in Nanyang cattle. Mol Biol Rep 38(3):2179–2184PubMedCrossRefGoogle Scholar
  14. 14.
    Adoligbe C, Zan L, Farougou S, Wang H, Ujjan JA (2012) Bovine GDF10 gene polymorphism analysis and its association with body measurement traits in Chinese indigenous cattle. Mol Biol Rep 39(4):4067–4075PubMedCrossRefGoogle Scholar
  15. 15.
    Wang J, Li ZJ, Lan XY, Hua LS, Huai YT, Huang YZ, Ma L, Zhao M, Jing YJ, Chen H, Wang JQ (2010) Two novel SNPs in the coding region of the bovine PRDM16 gene and its associations with growth traits. Mol Biol Rep 37(1):571–577PubMedCrossRefGoogle Scholar
  16. 16.
    Joseph S, David WR (2002) In: Huang PT (ed) Molecular cloning a laboratory manual, 3rd edn. Science Press, BeijingGoogle Scholar
  17. 17.
    Gilbert RP, Bailey DRC, Shannon NH (1993) Linear body measurements of cattle before and after 20 years of selection for postweaning gain when fed two different diets. J Anim Sci 71(7):1712–1720PubMedGoogle Scholar
  18. 18.
    Nei M, Roychoudhurg AK (1974) Sampling variance of heterozygosity and genetic distance. Genetics 76(2):379–390PubMedGoogle Scholar
  19. 19.
    Crow LF, Kimura MA (1970) Introduction to population genetics theory. Harper & Row, New YorkGoogle Scholar
  20. 20.
    Shi YY, He L (2005) SHEsis, a powerful software platform for analyses of linkage disequilibrium, haplotype construction, and genetic association at polymorphism loci. Cell Res 15(2):97–98PubMedCrossRefGoogle Scholar
  21. 21.
    Institute SAS (1999) Statistical analysis systems user’s guide (Version 8). SAS Institute, Inc., CaryGoogle Scholar
  22. 22.
    Tank PA, Pomp D (1994) Rapid communication: PCR-based Sau96I polymorphism in the bovine lipoprotein lipase gene. J Anim Sci 72(11):3032PubMedGoogle Scholar
  23. 23.
    Lien S, Gomez-Raya L, Vage DI (1995) A BsmAI polymorphism in the bovine lipoprotein lipase gene. Anim Genet 26(4):283–284PubMedGoogle Scholar
  24. 24.
    Guo YQ, Xu SZ, Song BZ, Gao X, Ren HY, Chen JB (2007) Genetic variation of LPL gene and association analysis with meat quality traits in bovine. Chinese J Agri Biotech 15(5):899–900Google Scholar
  25. 25.
    Rothschild MF, Soller M (1997) Candidate gene analysis to detect genes controlling traits of economic importance in domestic livestock. Probe 8:13–20Google Scholar
  26. 26.
    Li F, Chen H, Lei CZ, Ren G, Wang J, Li ZJ, Wang JQ (2010) Novel SNPs of the bovine NUCB2 gene and their association with growth traits in three native Chinese cattle breeds. Mol Biol Rep 37(1):541–546PubMedCrossRefGoogle Scholar
  27. 27.
    Liu YF, Zan LS, Li K, Zhao SP, Xin YP, Lin Q, Tian WQ, Wang ZW (2010) A novel polymorphism of GDF5 gene and its association with body measurement traits in Bos taurus and Bos indicus breeds. Mol Biol Rep 37(1):429–434PubMedCrossRefGoogle Scholar
  28. 28.
    Liu Y, Zan L, Zhao S, Xin Y, Li L, Cui W, Tang Z, Li K (2010) Molecular characterization, polymorphism of bovine ZBTB38 gene and association with body measurement traits in native Chinese cattle breeds. Mol Biol Rep 37(8):4041–4049PubMedCrossRefGoogle Scholar
  29. 29.
    Xue M, Zan LS, Gao L, Wang HB (2011) A novel polymorphism of the myogenin gene is associated with body measurement traits in native Chinese breeds. Genet Mol Res 10(4):2721–2728PubMedCrossRefGoogle Scholar
  30. 30.
    Hou GY, Yuan ZR, Zhou HL, Zhang LP, Li JY, Gao X, Wang DJ, Gao HJ, Xu SZ (2011) Association of thyroglobulin gene variants with carcass and meat quality traits in beef cattle. Mol Biol Rep 38(7):4705–4708PubMedCrossRefGoogle Scholar
  31. 31.
    Shin SC, Heo JP, Chung ER (2012) Genetic variants of the FABP4 gene are associated with marbling scores and meat quality grades in Hanwoo (Korean cattle). Mol Biol Rep 39(5):5323–5330PubMedCrossRefGoogle Scholar
  32. 32.
    Han SH, Cho IC, Ko MS, Kim EY, Park SP, Lee SS, Oh HS (2012) A promoter polymorphism of MSTN g.-371T>A and its associations with carcass traits in Korean cattle. Mol Biol Rep 39(4):3767–3772PubMedCrossRefGoogle Scholar
  33. 33.
    Huang M, Gao X, Li JY, Ren HY, Chen JB, Xu SZ (2010) Polymorphisms in MC4R gene and correlations with economic traits in cattle. Mol Biol Rep 37(8):3941–3944PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Xing Ping Wang
    • 1
  • Zhuo Ma Luoreng
    • 1
  • Feng Li
    • 1
  • Jin Ren Wang
    • 1
  • Na Li
    • 1
  • Shu Hong Li
    • 1
  1. 1.Key Laboratory of Zoology in Hunan Higher Education, College of Life ScienceHunan University of Arts and ScienceChangdeChina

Personalised recommendations