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Halopriming mediated salt and iso-osmotic PEG stress tolerance and, gene expression profiling in sugarcane (Saccharum officinarum L.)

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Abstract

Seed priming is a well known pre-germination strategy that improves seed performance. However, biochemical and molecular mechanisms underlying priming mediated stress tolerance are little understood. Here, we report results of the study on growth, physiological characteristics and expression of stress responsive genes in salt primed sugarcane cv. Co 86032 plants in response to salt (NaCl, 150 mM) or iso-osmotic (−0.7 MPa) polyethylene glycol-PEG 8000 (20 % w/v) stress exposure for 15 days. Variable growth, osmolyte accumulation and antioxidant capacity was revealed among the primed and non-primed plants. The primed plants showed better tolerance to the salt or PEG stress, as revealed by better growth and lower membrane damage, through better antioxidant capacity as compared to the respective non-primed controls. Further, steady state transcript expression analysis revealed up regulation of sodium proton antiporter (NHX) while, down regulation of sucrose transporter (SUT1), delta 1-pyrolline-5-carboxylate synthetase (P5CS) and proline dehydrogenase (PDH) in primed plants on exposure to the stress as compared to the non-primed plants. Transcript abundance of catalase (CAT2) decreased by about 25 % in leaves of non-primed stressed plants, however, the expression was maintained in leaves of the stressed primed plants to that of non-stressed controls. Thus, the results indicated priming mediated salt and PEG stress tolerance through altered gene expression leading to improved antioxidant capacity in sugarcane.

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Acknowledgments

Thanks are extended to University Grant Commission, New Delhi, India and Department of Botany, University of Pune, Pune, India for financial support in terms of Research Fellowship to senior author.

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  1. Vikas Yadav Patade

    Present address: Molecular Biology and Genetic Engineering Division, Defence Institute of Bio-Energy Research, Haldwani, Nainital, 263 139, India

Authors and Affiliations

  1. Functional Plant Biology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400 085, India

    Vikas Yadav Patade & Penna Suprasanna

  2. Botany Department, University of Pune, Pune, 411 007, India

    Vikas Yadav Patade & Sujata Bhargava

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  1. Vikas Yadav Patade
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Correspondence to Penna Suprasanna.

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Patade, V.Y., Bhargava, S. & Suprasanna, P. Halopriming mediated salt and iso-osmotic PEG stress tolerance and, gene expression profiling in sugarcane (Saccharum officinarum L.). Mol Biol Rep 39, 9563–9572 (2012). https://doi.org/10.1007/s11033-012-1821-7

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