Characterization of a novel ERF transcription factor in Artemisia annua and its induction kinetics after hormones and stress treatments
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The full-length cDNA sequence of AaERF3 was cloned and characterized from Artemisia annua. The bioinformatic analysis and phylogenetic tree analysis implied that the AaERF3 encoded a putative protein of 193 amino acids which formed a closely related subgroup with AtERF1, ERF1 and ORA59 in Arabidopsis. The result of subcellular localization showed that AaERF3 targeted to both of the nuclei and the cytoplasm. The qRT-PCR analysis showed that Green young alabastrums had the highest expression level of AaERF3 in the 5-months-old plants. The qRT-PCR analysis also revealed that ABA, Wound and Cold treatments significantly enhanced the transcript expression of AaERF3. MeJA and Ethylene treatment could also slightly induce the accumulation of AaERF3 transcription.
KeywordsEthylene response factor Induction kinetics Subcelluar localization
This work was funded by China “863” Program (grant no. 2011AA100605), China Transgenic Research Program (grant no. 2011ZX08002001), Ministry of Education, Shanghai Science and Technology Committee (Grant no. 08391911800) and Shanghai Leading Academic Discipline Project (Grant no. B209).
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