Characterization of a novel ERF transcription factor in Artemisia annua and its induction kinetics after hormones and stress treatments
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The full-length cDNA sequence of AaERF3 was cloned and characterized from Artemisia annua. The bioinformatic analysis and phylogenetic tree analysis implied that the AaERF3 encoded a putative protein of 193 amino acids which formed a closely related subgroup with AtERF1, ERF1 and ORA59 in Arabidopsis. The result of subcellular localization showed that AaERF3 targeted to both of the nuclei and the cytoplasm. The qRT-PCR analysis showed that Green young alabastrums had the highest expression level of AaERF3 in the 5-months-old plants. The qRT-PCR analysis also revealed that ABA, Wound and Cold treatments significantly enhanced the transcript expression of AaERF3. MeJA and Ethylene treatment could also slightly induce the accumulation of AaERF3 transcription.
KeywordsEthylene response factor Induction kinetics Subcelluar localization
This work was funded by China “863” Program (grant no. 2011AA100605), China Transgenic Research Program (grant no. 2011ZX08002001), Ministry of Education, Shanghai Science and Technology Committee (Grant no. 08391911800) and Shanghai Leading Academic Discipline Project (Grant no. B209).
- 6.Chen YY, Wang LF, Dai LJ, Yang SG, Tian WM (2011) Characterization of HbEREBP1, a wound-responsivetranscription factor gene in laticifers of Hevea brasiliensis Muell. Arg Mol Biol Rep. doi: 10.1007/s11033-011-1146-y
- 10.Lu X, Zhang FY, Jiang WM, Lin XY, Chen YF, Shen Q, Wang T, Wu SY, Sun XF, Tang KX (2011) Characterization of the first specific jasmonate biosynthetic pathway gene allene oxide synthase from Artemisia annua. Mol Biol Rep. doi: 10.1007/s11033-011-0976-y
- 13.Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Bio Evol 4:406–425Google Scholar
- 20.McGrath KC, Dombrecht B, Manners JM, Schenk PM, Edgar CI, Maclean DJ, Scheible WR, Udvardi MK, Kazan K (2005) Repressor- and activator-type ethylene response factors functioning in jasmonate signaling and disease resistance identified via a genome-wide screen of arabidopsis transcription factor gene expression. Plant Physiol 139:949–959PubMedCrossRefGoogle Scholar