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Molecular Biology Reports

, Volume 39, Issue 10, pp 9485–9491 | Cite as

Association study of ACE polymorphisms and systemic lupus erythematosus in Northern Chinese Han population

  • Ai-min Gong
  • Xin-yuan Li
  • Yi-qin Wang
  • Hai-xia Yan
  • Zhao-xia Xu
  • Zhao Feng
  • Yi-qiang Xie
  • De-hui Yin
  • Shi-zhong Yang
Article

Abstract

Systemic lupus erythematosus (SLE) is an autoimmune disease, with multiple genetic and environmental factors involving in its etiology. Angiotensin converting enzyme (ACE) gene was reported to have important roles in the development and progression of SLE. In this study, a case–control study was carried out to investigate the effects of seven SNPs and I/D in ACE gene in the development of SLE in Northern China. Seven SNPs including A5466C, T3892C, A240T, C1237T, G2215A, A2350G and C3409T were genotyped by PCR-RFLP method, and I/D was examined by agarose gel electrophoresis followed PCR directly. 314 SLE patients were compared to 320 normal controls in the study. Data were analyzed by SPSS 13.0 and HaploView software. The frequency distribution of SNP A2350G and Alu I/D and five haplotypes (AAAACCCI, AGAACCTD, AAAATCTI, TAAATTTI and TAAATCTI) were demonstrated to be different between case and control groups significantly. Whereas other SNPs and haplotypes had no differences in two cohorts. The results revealed that variations of ACE gene had association with SLE, which indicated ACE gene may play an important role in pathogenesis of SLE in Northern Chinese Han population.

Keywords

ACE SLE Polymorphism Association 

Notes

Acknowledgments

We greatly acknowledge the numerous donors for making this work possible. We thank the corresponding people of Harbin Medical University, Hainan Medical University and Shanghai University of Traditional Chinese.

Conflict of interest

None

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Ai-min Gong
    • 1
  • Xin-yuan Li
    • 2
  • Yi-qin Wang
    • 3
  • Hai-xia Yan
    • 3
  • Zhao-xia Xu
    • 3
  • Zhao Feng
    • 1
  • Yi-qiang Xie
    • 1
  • De-hui Yin
    • 4
  • Shi-zhong Yang
    • 4
  1. 1.Department of Internal Medicine of Traditional Chinese MedicineHainan Medical UniversityHaikouPeople’s Republic of China
  2. 2.Department of RheumatologyThe Second Affiliated Hospital of Harbin Medical UniversityHarbinPeople’s Republic of China
  3. 3.School of Basic MedicineShanghai University of Traditional ChineseShanghaiPeople’s Republic of China
  4. 4.Department of Health Preservation and RehabilitationHainan Medical UniversityHaikouPeople’s Republic of China

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