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Molecular Biology Reports

, Volume 39, Issue 9, pp 9105–9111 | Cite as

Replication study of PLCE1 and C20orf54 polymorphism and risk of esophageal cancer in a Chinese population

  • Haiyong Gu
  • Guowen Ding
  • Wenbo Zhang
  • Chao Liu
  • Yijang Chen
  • Suocheng Chen
  • Pengcheng Jiang
Article

Abstract

Esophageal cancer is one of the most aggressive cancers in the world. Recent large-scale genome-wide association studies (GWAS) reported that functional genetic variations in the phospholipase C epsilon gene (PLCE1) were strongly associated with risk of esophageal squamous cell carcinoma (ESCC) and gastric cardia adenocarcinoma (GCA) in Chinese population. For C20orf54 rs13042395 genotype and risk of esophageal cancer, the results were inconsistent. We conducted a replication case–control study to evaluate the genetic effects of these two functional single nucleotide polymorphisms (SNPs) on the development of esophageal cancer. A total of 380 cases and 380 controls were recruited for this study. The genotypes were determined by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF MS). The variant alleles of the functional polymorphism, PLCE1 rs2274223 SNP was associated with the increased risk of esophageal cancer [adjusted odds ratio (OR) = 1.95, 95 % confidence interval (CI) = 1.05–3.59 for PLCE1 rs2274223 GG vs. AA]. However, there was no significant association between the C20orf54 rs13042395 genotype and esophageal cancer risk (adjusted OR = 0.99, 95 % CI = 0.63–1.57 for C20orf54 rs13042395 TT vs. CC). Stratified analyses indicated a significantly increased risk of esophageal cancer associated with the PLCE1 rs2274223 AG genotype was more evident among females, younger patients and never drinkers, compared with the PLCE1 rs2274223 AA genotypes. Stratified analyses also indicated a significantly increased risk of esophageal cancer associated with the PLCE1 rs2274223 GG genotype was more evident among never smokers and never drinkers compared with the PLCE1 rs2274223 AA genotypes. These findings indicated that functional polymorphisms PLCE1 rs2274223 might contribute to esophageal cancer susceptibility.

Keywords

PLCE1, C20orf54 Polymorphisms Esophageal cancer Molecular epidemiology 

Abbreviations

CI

Confidential interval

GWAS

Genome-wide association study

LD

Linkage disequilibrium

OR

Odds ratio

SNPs

Single nucleotide polymorphisms

Notes

Acknowledgments

This study was supported in part by National Natural Science Foundation of China (81101889) and Social Development Foundation of Zhenjiang (SH2010017).

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  1. 1.Department of Cardiothorac SurgeryAffiliated People’s Hospital of Jiangsu UniversityZhenjiangChina
  2. 2.Department of Thoracic & Cardiac SurgeryThe First Affiliated Hospital of Nanjing Medical UniversityNanjingChina
  3. 3.Department of General SurgeryAffiliated People’s Hospital of Jiangsu UniversityZhenjiangChina

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