Genetic polymorphisms of glutathione S-transferase Z1 (GSTZ1) and susceptibility to preeclampsia
- 186 Downloads
Preeclampsia (PE) is a complex disorder affected by genetic and environmental factors. Although the exact genes involved in development of PE are still not fully discovered, an important role for oxidative stress in its pathogenesis is accepted. In the present study, the association between the functional genetic polymorphisms in codons 32, 42 and nucleotide −1002 of glutathione S-transferases Z1 (GSTZ1) and susceptibility to PE was investigated. The present case–control study was performed on 151 preeclapmtic patients, and a total of 200 normal pregnant women, as a control group. The healthy control group was frequency matched with the age of the preeclamptic patients. Control subjects had no history of previous pregnancies with PE. Genotypes were determined by PCR–RFLP assay. There was no significant association between G-1002A and Glu32Lys polymorphisms of GSTZ1 with PE risk. The variant allele of Gly42Arg polymorphism decreased the risk of PE (OR = 0.24, 95 % CI 0.08–0.73, P = 0.012). The haplotype of “−1002A, 32Lys, 42Arg” (having three variant alleles) versus to the other haplotypes significantly decreased among PE patients compared to the control group (5.0 vs. 0.9 percent among control and PE patient groups, respectively; χ2 = 9.328, df = 1, P = 0.002). The present results indicate that the haplotype of “−1002A, 32Lys, 42Arg” (containing three variant alleles) of GSTZ1 have protective effect compared to the other haplotypes.
KeywordsGSTZ1 Polymorphism Preeclampsia Haplotype
The authors are indebted to the participants for their close cooperation. This study was supported by Shiraz University.
Conflict of interest
- 8.Gebhardt GS, Peters WH, Hillermann R, Odendaal HJ, Carelse-Tofa K, Raijmakers MT, Steegers EA (2004) Maternal and fetal single nucleotide polymorphisms in the epoxide hydrolase and gluthatione S-transferase P1 genes are not associated with pre-eclampsia in the Coloured population of the Western Cape, South Africa. J Obstet Gynaecol 24:866–872CrossRefPubMedGoogle Scholar
- 10.Ohta K, Kobashi G, Hata A, Yamada H, Minakami H, Fujimoto S, Kondo K, Tamashiro H (2003) Association between a variant of the glutathione S-transferase P1 gene (GSTP1) and hypertension in pregnancy in Japanese: interaction with parity, age, and genetic factors. Semin Thromb Hemost 29:653–659CrossRefPubMedGoogle Scholar
- 12.Zhang J, Masciocchi M, Lewis D, Sun W, Liu A, Wang Y (2008) Placental anti-oxidant gene polymorphisms, enzyme activity, and oxidative stress in preeclampsia. Placenta 29:343–439Google Scholar
- 15.Anvar Z, Saadat I, Namavar-Jahromi B, Saadat M (2011) Genetic polymorphisms of glutathione S-transferase M1 (GSTM1) and T1 (GSTT1) and susceptibility to pre-eclampsia: a case-control study and a meta-analysis. EXCLI J 10:44–51Google Scholar
- 19.Newton CR (1995) Mutational analysis: known mutations. In: McPherson MJ, Hames D, Taylor GR (eds) PCR2. A practical approach. IRL Press, Oxford, pp 219–222Google Scholar
- 20.Andonova IE, Justenhoven C, Winter S, Hamann U, Baisch C, Rabstein S, Spickenheuer A, Harth V, Pesch B, Brüning T, Ko YD, Ganev V, Brauch H (2010) No evidence for glutathione S-transferases GSTA2, GSTM2, GSTO1, GSTO2, and GSTZ1 in breast cancer risk. Breast Cancer Res Treat 121:497–502CrossRefPubMedGoogle Scholar
- 24.Othman H, Saadat I, Farvardin-Jahromi M, Saadat M (2012) Susceptibility to exudative age-related macular degeneration and three genetic polymorphisms of glutathione S-transferase Z1 (GSTZ1). Eur J Ophthalmol 22:431–435Google Scholar
- 25.Saadat I, Khalili M, Nafissi S, Omidvari S, Saadat M (2012) Susceptibility to breast cancer and three polymorphisms of GSTZ1. DNA Cell Biol 31:337–341Google Scholar