Molecular Biology Reports

, Volume 39, Issue 9, pp 8837–8843 | Cite as

Interleukin-8 and -10 gene polymorphisms in irritable bowel syndrome

  • Mirza Romero-Valdovinos
  • Areli Gudiño-Ramírez
  • Jesús Reyes-Gordillo
  • Williams Arony Martínez-Flores
  • María Elena Ramírez-Miranda
  • Pablo Maravilla
  • Angélica Olivo-Díaz


Irritable bowel syndrome (IBS) is one of the most common gastrointestinal diagnoses seen by primary care providers and gastroenterologists. Proinflammatory cytokines interleukin (IL)-6 and IL-8 have been found increased in IBS patients. Cytokine gene single nucleotide polymorphisms (SNPs) of IL-8 and IL-10 have not been assessed in Mexican IBS patients. DNA was extracted from peripheral blood leucocytes of 45 IBS unrelated patients and 137 controls. Allele, genotype, and haplotype frequencies were determined by analyzing SNPs of IL-8 and IL-10 genes. IL-8 + 396 G allele (P = 0.02), IL-8 + 396(G/G) and IL-8 + 781(C/T) genotypes (P < 0.001), IL-10 − 1082A allele and IL-10 − 1082(A/A) genotype (P < 0.001) were significantly increased in the IBS group. Haplotypes IL-8 ATCC (P = 0.03) and IL-10 ACC (P < 0.001) were associated with susceptibility to develop IBS. An association of certain polymorphisms of IL-8 and IL-10 in IBS patients compared to controls was demonstrated, suggesting a role of these cytokine SNPs in the pathophysiology of IBS.


IL-8 IL-10 Irritable bowel syndrome Single nucleotide polymorphisms 



The authors would like to thank Ana Flisser for her critical comments; Maria Elena Rodriguez-Campa and Alberto Gonzalez-Angulo for their clinical assistance. Rocio Jimenez-Lucio and David Sierra performed DNA extractions. This work was partially supported by CONACYT Grant 69589.


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Mirza Romero-Valdovinos
    • 1
  • Areli Gudiño-Ramírez
    • 1
  • Jesús Reyes-Gordillo
    • 1
  • Williams Arony Martínez-Flores
    • 1
  • María Elena Ramírez-Miranda
    • 1
  • Pablo Maravilla
    • 1
  • Angélica Olivo-Díaz
    • 1
  1. 1.Dirección de InvestigaciónHospital General “Dr. Manuel Gea González”MexicoMexico

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