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Down-regulation of the CrHPT1 histidine phosphotransfer protein prevents cytokinin-mediated up-regulation of CrDXR, and CrG10H transcript levels in periwinkle cell cultures

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Abstract

In Catharanthus roseus cell cultures, cytokinins (CK) improve monoterpenoid indole alkaloids (MIAs) accumulation. This metabolite production is correlated with an increase of transcripts corresponding to genes encoding both elements of the CK-signaling pathway and enzymes implicated in MIAs biosynthesis. However, it has not been demonstrated that the CK signal, leading to MIAs accumulation, comes through components identified as belonging to the CK-signaling pathway. In this work, we addressed this question, by transgenesis, using an inducible RNAi system targeting element of CK-signaling. In transgenic lines, the up-regulation by CK of two genes involved in MIA biosynthesis was abolished. These results demonstrate a relationship between the CK-signaling and the MIAs biosynthetic pathways.

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References

  1. Amini A, Glévarec G, Andreu F, Reverdiau P, Rideau M, Creche J (2008) Effect of phosphatidic acid on cytokinin signal transduction in periwinkle cells. J Plant Growth Regul 27:394–399

    Article  CAS  Google Scholar 

  2. Amini A, Glévarec G, Andreu F, Rideau M, Crèche J (2009) Low levels of gibberellic acid control the biosynthesis of ajmalicine in Catharanthus roseus cell suspension cultures. Planta Med 75:187–191

    Article  PubMed  CAS  Google Scholar 

  3. Aoyama T, Chua NH (1997) A glucocorticoid-mediated transcriptional induction system in transgenic plants. Plant J 11:605–612

    Article  PubMed  CAS  Google Scholar 

  4. Argyros RD, Mathews DE, Chiang YH, Palmer CM, Thibault DM, Etheridge N, Argyros DA, Mason MG, Kieber JJ, Schaller GE (2009) Type B response regulators of Arabidopsis play key roles in cytokinin signaling and plant development. Plant Cell 20:2102–2116

    Google Scholar 

  5. Décendit A, Liu D, Ouelhazi L, Doireau P, Mérillon JM, Rideau M (1992) Cytokinin-enhanced accumulation of indole alkaloids in Catharanthus roseus cell cultures. The factors affecting the cytokinin response. Plant Cell Rep 11:400–403

    Article  Google Scholar 

  6. Gamborg OL, Miller RA, Ojima K (1968) Nutrients requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–168

    Article  PubMed  CAS  Google Scholar 

  7. Ginis O, Courdavault V, Melin C, Lanoue A, Giglioli-Guivarc’h N, St-Pierre B, Courtois M, Oudin A (2012) Molecular cloning and functional characterization of Catharanthus roseus hydroxymethylbutenyl 4-diphosphate synthase gene promoter from the methyl erythritol phosphate pathway. Mol Biol Rep. doi:10.1007/s11033-011-1343-8 (in press)

  8. Hutchison CE, Li J, Argueso C, Gonzalez M, Lee E, Lewis MW, Maxwell BB, Perdue TD, Schaller GE, Alonso JM, Ecker JR, Kieber JJ (2006) The Arabidopsis histidine phosphotransfer proteins are redundant positive regulators of cytokinin signaling. Plant Cell 18:3073–3087

    Article  PubMed  CAS  Google Scholar 

  9. Ishida K, Yamashino T, Yokoyama A, Mizuno T (2008) Three type-B response regulators, ARR1, ARR10 and ARR12, play essential but redundant roles in cytokinin signal transduction throughout the life cycle of Arabidopsis thaliana. Plant Cell Physiol 49:47–57

    Article  PubMed  CAS  Google Scholar 

  10. Mason MG, Li J, Mathews DE, Kieber JJ, Schaller GE (2004) Type-B response regulators display overlapping expression patterns in Arabidopsis. Plant Physiol 135:927–937

    Article  PubMed  CAS  Google Scholar 

  11. Menke FL, Champion A, Kijne JW, Memelink J (1999) A novel jasmonate- and elicitor-responsive element in the periwinkle secondary metabolite biosynthetic gene Str interacts with a jasmonate- and elicitor-inducible AP2-domain transcription factor, ORCA2. EMBO J 18:4455–4463

    Article  PubMed  CAS  Google Scholar 

  12. Mok DW, Mok MC (2001) Cytokinin metabolism and action. Annu Rev Plant Physiol Plant Mol Biol 52:89–118

    Article  PubMed  CAS  Google Scholar 

  13. Papon N, Oudin A, Clastre M, Rideau M, Chénieux JC, Crèche J (2002) Isolation of CrHPT1, a cDNA encoding a histidine-containing phosphotransfer domain in Catharanthus roseus. Acta Bot Gallica 149:67–77

    CAS  Google Scholar 

  14. Papon N, Clastre M, Gantet P, Rideau M, Chénieux JC, Crèche J (2003) Inhibition of the plant cytokinin transduction pathway by bacterial histidine kinases inhibitors in Catharanthus roseus cell cultures. FEBS Lett 537:101–105

    Article  PubMed  CAS  Google Scholar 

  15. Papon N, Oudin A, Vansiri A, Rideau M, Chénieux JC, Crèche J (2003) Differential expression of two type-A response regulators in plant and cell cultures of Catharanthus roseus. J Exp Bot 54:1793–1795

    Article  PubMed  CAS  Google Scholar 

  16. Papon N, Vansiri A, Gantet P, Chenieux JC, Rideau M, Creche J (2004) Histidine-containing phosphotransfer domain extinction by RNA interference turns off a cytokinin signaling circuitry in Catharanthus roseus suspension cells. FEBS Lett 558:85–88

    Article  PubMed  CAS  Google Scholar 

  17. Papon N, Bremer J, Vansiri A, Andreu F, Rideau M, Creche J (2005) Cytokinin and ethylene control indole alkaloid production at the level of the MEP/terpenoid pathway in Catharanthus roseus suspension cells. Planta Med 71:572–574

    Article  PubMed  CAS  Google Scholar 

  18. Rashotte AM, Mason MG, Hutchison CE, Ferreira FJ, Schaller GE, Kieber JJ (2006) A subset of Arabidopsis AP2 transcription factors mediates cytokinin responses in concert with a two-component pathway. Proc Natl Acad Sci USA 103:11081–11085

    Article  PubMed  CAS  Google Scholar 

  19. Thabet I, Guirimand G, Guihur A, Lanoue A, Courdavault V, Papon N, Bouzid S, Giglioli-Guivarc’h N, Simkin AJ, Clastre M (2012) Characterization and subcellular localization of geranylgeranyl diphosphate synthase from Catharanthus roseus. Mol Biol Rep 39:3235–3243

    Article  PubMed  CAS  Google Scholar 

  20. To JP, Haberer G, Ferreira FJ, Deruere J, Mason MG, Schaller GE, Alonso JM, Ecker JR, Kieber JJ (2004) Type-A Arabidopsis response regulators are partially redundant negative regulators of cytokinin signaling. Plant Cell 16:658–671

    Article  PubMed  CAS  Google Scholar 

  21. To JP, Kieber JJ (2008) Cytokinin signaling: two-components and more. Trends Plant Sci 13:85–92

    Article  PubMed  CAS  Google Scholar 

  22. van Der Heijden R, Jacobs DI, Snoeijer W, Hallard D, Verpoorte R (2004) The Catharanthus alkaloids: pharmacognosy and biotechnology. Curr Med Chem 11:607–628

    Article  Google Scholar 

  23. Waterhouse PM, Helliwell CA (2003) Exploring plant genomes by RNA induced gene silencing. Nat Rev Genet 4:29–38

    Article  PubMed  CAS  Google Scholar 

  24. Yokoyama A, Yamashino T, Amano Y, Tajima Y, Imamura A, Sakakibara H, Mizuno T (2007) Type-B ARR transcription factors, ARR10 and ARR12, are implicated in cytokinin-mediated regulation of protoxylem differentiation in roots of Arabidopsis thaliana. Plant Cell Physiol 48:84–96

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This research was supported by the Ministère de l’Education Nationale et de la Technologie (MENRT, France). A doctoral grant was awarded by the MENRT to AA. Authors are grateful to N. H. Chua for providing the vector PTA-7001.

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  1. Aniça Amini

    Present address: Department of Plant Biology & Pathology, Rutgers The State University of New Jersey, 59 Dudley Road, New Brunswick, NJ, 08901, USA

Authors and Affiliations

  1. EA 2106 Biomolécules et Biotechnologies Végétales, Faculté de Pharmacie, Université François Rabelais de Tours, 31 Avenue Monge, 37200, Tours, France

    Aniça Amini, Françoise Andreu, Gaëlle Glévarec, Marc Rideau & Joël Crèche

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  1. Aniça Amini
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  2. Françoise Andreu
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  3. Gaëlle Glévarec
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  4. Marc Rideau
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  5. Joël Crèche
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Correspondence to Joël Crèche.

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Amini, A., Andreu, F., Glévarec, G. et al. Down-regulation of the CrHPT1 histidine phosphotransfer protein prevents cytokinin-mediated up-regulation of CrDXR, and CrG10H transcript levels in periwinkle cell cultures. Mol Biol Rep 39, 8491–8496 (2012). https://doi.org/10.1007/s11033-012-1703-z

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  • DOI: https://doi.org/10.1007/s11033-012-1703-z

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