Molecular Biology Reports

, Volume 39, Issue 8, pp 8491–8496 | Cite as

Down-regulation of the CrHPT1 histidine phosphotransfer protein prevents cytokinin-mediated up-regulation of CrDXR, and CrG10H transcript levels in periwinkle cell cultures

  • Aniça Amini
  • Françoise Andreu
  • Gaëlle Glévarec
  • Marc Rideau
  • Joël Crèche


In Catharanthus roseus cell cultures, cytokinins (CK) improve monoterpenoid indole alkaloids (MIAs) accumulation. This metabolite production is correlated with an increase of transcripts corresponding to genes encoding both elements of the CK-signaling pathway and enzymes implicated in MIAs biosynthesis. However, it has not been demonstrated that the CK signal, leading to MIAs accumulation, comes through components identified as belonging to the CK-signaling pathway. In this work, we addressed this question, by transgenesis, using an inducible RNAi system targeting element of CK-signaling. In transgenic lines, the up-regulation by CK of two genes involved in MIA biosynthesis was abolished. These results demonstrate a relationship between the CK-signaling and the MIAs biosynthetic pathways.


Cytokinin Catharanthus roseus Monoterpenoid indole alkaloids Gene regulation Inducible RNAi 



This research was supported by the Ministère de l’Education Nationale et de la Technologie (MENRT, France). A doctoral grant was awarded by the MENRT to AA. Authors are grateful to N. H. Chua for providing the vector PTA-7001.


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Aniça Amini
    • 1
    • 2
  • Françoise Andreu
    • 1
  • Gaëlle Glévarec
    • 1
  • Marc Rideau
    • 1
  • Joël Crèche
    • 1
  1. 1.EA 2106 Biomolécules et Biotechnologies Végétales, Faculté de PharmacieUniversité François Rabelais de ToursToursFrance
  2. 2.Department of Plant Biology & PathologyRutgers The State University of New JerseyNew BrunswickUSA

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